FastQCFastQC Report
Thu 11 Jan 2018
H57VTBCX2_l02n01_d10_g5_br.351000000b8a38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VTBCX2_l02n01_d10_g5_br.351000000b8a38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4034326
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT96230.2385280713556614No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT78570.19475372094372145TruSeq Adapter, Index 13 (97% over 40bp)
CTCACACCGATCTAAACTCAAATCATGTAAGATTTTAATAATCGAACAGA69470.172197288964749No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC67480.16726461867484185No Hit
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTCACACCGA61230.1517725637442289No Hit
CTGGCTCACACCGATCTAAACTCAAATCATGTAAGATTTTAATAATCGAA51970.12881953515903277No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT47830.11855759797299474No Hit
GTTAAAAGAAAAGAAACTTTAATAAATATTTTTTCCATAGATGAATGATT46000.11402152428931127No Hit
CTCATTTTAAACCTTAAATACTCTACATTTTGATTTGATTTCTTATATTT43550.10794863875651099No Hit
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT42160.10450320573994268No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA26600.022.9066664
GTATGCC20650.020.12497546-47
GTCATAA32350.019.7134045
TAATCCC33750.019.1375169
TATGCCG22450.018.9345148-49
GAGGTCA30350.018.5078892
TAAACCT24350.018.1386288
CTCATTT31800.017.9709571
GCCGTCT24050.017.77346650-51
AGGTCAT32300.017.6852933
TTCGTAC62500.017.6702023
TTAAACC27100.017.5247237
CCAGTCA24750.016.9826226-27
CGTACAA65400.016.9554465
CGTATGC25600.016.69744146-47
CTTTCGT71600.016.6946661
ATGCCGT25950.016.47223548-49
AGTCAAC26300.016.43259434-35
CAGTCAC25600.016.41874326-27
CACAGTC25500.016.39000530-31
Produced by FastQC (version 0.11.2)