FastQCFastQC Report
Thu 11 Jan 2018
H57VTBCX2_l02n01_d10_g4_ov.351000000b8a1e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VTBCX2_l02n01_d10_g4_ov.351000000b8a1e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2114914
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC208530.9859975393798518TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG33100.030.34950346-47
ATGCCGT34200.028.88726446-47
GCTTGAA34800.028.45946958-59
TGCTTGA35150.028.2436658-59
CCGTCTT35450.028.202350-51
GCCGTCT35800.027.99390648-49
GTATGCC35900.027.717844-45
CCTTGTA36400.027.13844332-33
GAGCACA76250.026.5953629
AGTCACC37300.026.54823328-29
AGAGCAC76550.026.1809338
GTCACCT37650.026.1752928-29
ACCTTGT38050.026.14883432-33
CTTGAAA38650.026.05471660-61
CTCGTAT38450.026.0030942-43
CGTATGC38550.025.9972444-45
CAGTCAC38300.025.79306426-27
TAATCTC38800.025.76730738-39
TCGTATG38650.025.68419642-43
TGTAATC39150.025.59669736-37
Produced by FastQC (version 0.11.2)