FastQCFastQC Report
Thu 11 Jan 2018
H57VTBCX2_l02n01_d10_g4_fb.351000000b89f3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VTBCX2_l02n01_d10_g4_fb.351000000b89f3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1931402
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC237431.2293142494415974TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC33750.034.75916744-45
ATGCCGT34200.034.23237246-47
TCGTATG34700.033.26005642-43
GCCGTCT35150.033.1044948-49
CCGTCTT36300.032.709550-51
ACGGCTA36200.032.470632-33
CTTGAAA36950.031.68545260-61
AGAGCAC74800.031.2382858
GAGCACA75300.031.1579
TATGCCG38500.030.84078646-47
CACGGCT38700.030.25107830-31
TCACGGC39200.030.04695930-31
CTCGTAT39100.030.00311742-43
CGGCTAC39200.029.8644532-33
GTCACGG39750.029.39263328-29
ACATCTC40100.029.37182638-39
GCTTGAA40350.029.07440458-59
TCTGCTT41300.028.92163556-57
TCTCGTA40250.028.9095140-41
TGCTTGA41550.028.6919558-59
Produced by FastQC (version 0.11.2)