FastQCFastQC Report
Thu 11 Jan 2018
H57VTBCX2_l02n01_d10_g4_br.351000000b89cc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VTBCX2_l02n01_d10_g4_br.351000000b89cc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1714134
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC186011.0851543694950336TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC29400.032.47109244-45
TAGCTTA29200.032.1223334-35
CACTAGC30050.031.45261430-31
TATGCCG30500.030.91069846-47
ACTAGCT30300.030.87870232-33
CTAGCTT30550.030.78147532-33
TGCTTGA30600.030.73117858-59
TCACTAG31100.030.2379930-31
AGCTTAT31200.030.21542734-35
ATGCCGT31750.029.6937446-47
GAGCACA65250.029.5507359
GCTTGAA32300.029.48134658-59
GTCACTA32300.029.33516328-29
CCGTCTT32900.028.94369350-51
CGTATGC32850.028.84401344-45
GCTTATC32850.028.62588736-37
GCCGTCT33800.028.45444548-49
AGAGCAC66900.028.3959678
TCGTATG33400.028.22683542-43
CTTATCT33550.028.02862536-37
Produced by FastQC (version 0.11.2)