FastQCFastQC Report
Thu 11 Jan 2018
H57VTBCX2_l02n01_d10_g3_ov.351000000b89a3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VTBCX2_l02n01_d10_g3_ov.351000000b89a3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2231008
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC94900.4253682640313257TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG20950.021.76330646-47
GTATGCC21600.021.10839344-45
CGTATGC23000.020.44301844-45
ATGCCGT22750.019.83261146-47
GCCGTCT22600.019.64901748-49
GCTTGAA25550.018.4031358-59
TCGTATG24800.018.28897542-43
TGCTTGA25800.018.22480458-59
CCGTCTT27400.017.24686650-51
AGTCACG27350.017.0184928-29
GAGCACA55350.016.7315129
AGATCTC28600.016.60625338-39
AGAGCAC57200.016.4394538
CCAGTCA27750.016.43105126-27
CAGATCT28250.016.3915136-37
TGCCGTC28600.016.27412848-49
CTCGTAT29950.015.69914642-43
CTTGAAA29900.015.56690560-61
GATCAGA30300.015.28234234-35
TCAGATC30700.015.23809436-37
Produced by FastQC (version 0.11.2)