FastQCFastQC Report
Thu 11 Jan 2018
H57VTBCX2_l02n01_d10_g3_fb.351000000b8989.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VTBCX2_l02n01_d10_g3_fb.351000000b8989.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2387660
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC127120.5324041111381017TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT23400.026.79035246-47
CGTATGC24700.025.38033544-45
TCGTATG26350.023.79105442-43
GCCGTCT26750.023.52406948-49
ACACTTG28600.021.75355132-33
CTCGTAT28900.021.60967842-43
TCACACT28800.021.52025830-31
TATGCCG29300.021.47675346-47
GTCACAC29250.021.10821528-29
CACACTT30000.020.81775930-31
TCTCGTA30600.020.4867440-41
GAGCACA63150.020.4557729
CTTGAAA31050.020.11377560-61
TGCTTGA32300.020.07056458-59
GCTTGAA31700.020.00098858-59
AGAGCAC63250.019.9729168
TGAATCT31450.019.93304636-37
CCGTCTT33300.019.2535150-51
CTGCTTG32800.019.11303156-57
GTATGCC34500.018.92796544-45
Produced by FastQC (version 0.11.2)