FastQCFastQC Report
Thu 11 Jan 2018
H57VTBCX2_l02n01_d10_g3_br.351000000b897c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VTBCX2_l02n01_d10_g3_br.351000000b897c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2332948
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC185340.7944454827111449TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA63900.030.025399
GTATGCC31900.029.62837244-45
TATGCCG33300.028.38273646-47
AGAGCAC67650.028.2206128
ATGCCGT36100.026.31286846-47
ACCAGAT36550.026.18270132-33
TGCTTGA36500.026.15407258-59
CGTATGC36250.026.13847744-45
TCGTATG37350.025.62326642-43
CAGATCA37300.025.400534-35
CCAGTCA37900.024.93786426-27
GCCGTCT37950.024.90447448-49
AGTCACC38250.024.77175928-29
CCGTCTT39000.024.35522750-51
AAGAGCA77950.024.308887
CACCAGA39350.024.2603330-31
GCTTGAA39500.024.10756758-59
CAGTCAC39700.023.86699726-27
CTCGTAT40300.023.86547342-43
TCACCAG40650.023.4260630-31
Produced by FastQC (version 0.11.2)