FastQCFastQC Report
Thu 11 Jan 2018
H57VTBCX2_l02n01_d10_g2_ov.351000000b8953.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VTBCX2_l02n01_d10_g2_ov.351000000b8953.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1831084
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC86580.47283467061041434TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG20550.018.9507746-47
ATGCCGT21150.017.85178446-47
GCCGTCT21700.017.50874948-49
CCGTCTT21850.017.38854850-51
CGCCCAT9200.017.0829281
GCTTGAA22250.016.97014858-59
TGCTTGA22800.016.76909358-59
CACGCCA23600.016.30127330-31
CGTATGC23500.016.268744-45
GCCAATA23350.016.16981534-35
CTGCTTG24500.015.99258356-57
TCGTATG23950.015.86387542-43
GTATGCC24350.015.7983244-45
ACGCCAA24350.015.603703532-33
CTTGAAA25050.015.35768860-61
CGCCAAT24750.015.35152332-33
AGTCACG24550.015.28354628-29
CTCGTAT26500.015.23343642-43
TCACGCC24950.015.22888130-31
GTCACGC24950.015.133728-29
Produced by FastQC (version 0.11.2)