FastQCFastQC Report
Thu 11 Jan 2018
H57VTBCX2_l02n01_d10_g2_fb.351000000b8939.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VTBCX2_l02n01_d10_g2_fb.351000000b8939.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2766525
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC132940.48053062958043036TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC27350.024.57214544-45
ATGCCGT27400.024.44063446-47
GACCACA7300.023.4707642
CCGTCTT30650.022.23624450-51
TCGTATG30300.022.17980842-43
GCCGTCT30000.022.16413348-49
CACAGTG30000.022.00561732-33
ACAGTGA30500.021.95571134-35
CACACAG31350.020.98282830-31
AGTGATC31850.020.87635436-37
GTCACAC32150.020.31297528-29
TATGCCG34950.020.24804146-47
CAGTGAT34400.019.53557834-35
GTATGCC37500.019.4411144-45
CTCGTAT34750.019.33951642-43
CCACATA9400.019.2399234
CTTGAAA34950.019.2295460-61
GCTTGAA35600.019.14527358-59
ACACAGT36200.018.76149732-33
TCACACA35200.018.75529730-31
Produced by FastQC (version 0.11.2)