FastQCFastQC Report
Thu 11 Jan 2018
H57VTBCX2_l02n01_d10_g2_br.351000000b891f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VTBCX2_l02n01_d10_g2_br.351000000b891f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2667366
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC145170.5442447718085932TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC28650.025.11378744-45
TATGCCG29550.024.18817946-47
ACTGACC30750.023.32256932-33
CTGACCA31400.022.99103432-33
CGTATGC31550.022.73012244-45
CCAATCT32050.022.52412236-37
ATGCCGT32750.022.18729246-47
TCGTATG32550.022.17792142-43
CACTGAC32900.022.08779130-31
TGACCAA33150.021.84834934-35
TGCTTGA32900.021.79865358-59
AGTCACT33750.021.6018728-29
TCACTGA33800.021.42939630-31
GCCGTCT34000.021.23190148-49
CCGTCTT35000.021.16804550-51
GACCAAT34500.020.99341434-35
GAGCACA68600.020.977369
CCAGTCA34700.020.87358926-27
ACCAATC35000.020.55781436-37
GTCACTG35500.020.33630228-29
Produced by FastQC (version 0.11.2)