FastQCFastQC Report
Thu 11 Jan 2018
H57VTBCX2_l02n01_d10_g1_ov.351000000b88e7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VTBCX2_l02n01_d10_g1_ov.351000000b88e7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1993280
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC103690.5201978648258148TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGG20400.024.44560632-33
TATGCCG21050.024.14139746-47
CTTAGGC20700.023.7471632-33
TAGGCAT20800.023.40406634-35
CACTTAG21400.022.85998730-31
CCGTCTT22850.021.82397550-51
ATGCCGT22750.021.81552346-47
GCTTGAA23300.021.40301958-59
TGCTTGA23950.021.0204558-59
GCCGTCT23800.020.85307548-49
TCACTTA24450.020.39684530-31
CGTATGC24950.020.36779444-45
TTAGGCA25350.020.14008334-35
GTATGCC25250.019.74961944-45
AGGCATC25800.019.51316836-37
CTGCTTG26100.019.37962356-57
GGCATCT26500.019.08733736-37
AGTCACT26150.019.07085428-29
TGCCGTC27000.018.82135248-49
CTCGTAT27850.018.67347542-43
Produced by FastQC (version 0.11.2)