FastQCFastQC Report
Thu 11 Jan 2018
H57VTBCX2_l02n01_d10_g1_fb.351000000b88cd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VTBCX2_l02n01_d10_g1_fb.351000000b88cd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2302960
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC64380.27955327057352275TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTAGG4001.8189894E-1219.0339455
CGGGTCT3651.1277734E-1018.260071
GACCACA6200.017.6555212
CGTATGC17900.017.64439844-45
ATGCCGT18750.016.46456746-47
CGGGGCA2657.776203E-616.1682831
TCGTATG19500.016.07487342-43
TCTAGGA4451.1277734E-1016.0164976
CCGTCTT19900.015.99042250-51
CCGGGGG2508.551733E-515.2341171
CCCGGTT2205.60763E-415.1475581
ATGTATC20800.014.95521436-37
GCCGTCT20500.014.59569948-49
CTTGAAA23600.014.18996660-61
CTCGTAT23150.013.84812642-43
TATGCCG24400.013.81995946-47
TGCTTGA23950.013.78426258-59
ACCACAT8300.013.7618723
CCACATA8450.013.5175794
ACATAAC9200.013.4283246
Produced by FastQC (version 0.11.2)