FastQCFastQC Report
Thu 11 Jan 2018
H57VTBCX2_l02n01_d10_g1_br.351000000b88a4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VTBCX2_l02n01_d10_g1_br.351000000b88a4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1170741
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC28720.24531471948108077TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAT8150.021.5620928
CGGGGCT2601.4515535E-820.1421621
GTATGCC8500.018.1605844-45
TCTCTTA9700.018.11667
TATGCCG9700.016.40356846-47
CGGGGAT5153.6379788E-1215.7155091
CTTTTCT18900.015.3657871
CGTATGC10850.014.88382244-45
TGCTTGA10400.014.84214958-59
ATGCCGT11050.014.18459546-47
GCCGTCT12000.013.85296748-49
TCTTATT14150.013.4261139
CTGGAAT6055.638867E-1113.3776641
GCTTGAA12300.013.321732558-59
CCGTCTT12200.013.23599450-51
CGGGCAT3255.334535E-513.183961
TCGTATG12150.013.09529542-43
TATCTAG2600.001987885212.8106095
AGTCACA12400.012.63976528-29
CTGGCAT4606.856517E-712.4196731
Produced by FastQC (version 0.11.2)