FastQCFastQC Report
Thu 11 Jan 2018
H57VTBCX2_l02n01_d0_4_ov.351000000b8ad8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VTBCX2_l02n01_d0_4_ov.351000000b8ad8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8730133
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAT70050.019.1867458
TCTCTTA79050.017.9034777
CGGGAAT41250.012.2388441
CTTTTCT174100.012.1736191
CGGGGTT13550.011.5993351
TCTTATT121750.011.1172899
ATTTCCC65150.011.08028412-13
CTGGGAT27000.010.9367031
TCTAGAC31100.010.8422887
CGGGGAT28350.010.5839051
CTCGAAT65200.010.3728771
CGGGGCT13150.010.14123251
CTAGACT32100.010.0606698
CTGGAAT53650.09.8539811
CTCAGAT38200.09.4756431
CGGGGAG27700.09.2847991
CTAGAAT45150.09.2828941
CTTGAAT75400.09.2223161
CTCGGAT42900.09.2146591
CTCAGTT34850.09.1565211
Produced by FastQC (version 0.11.2)