FastQCFastQC Report
Thu 11 Jan 2018
H57VTBCX2_l02n01_d0_4_fb.351000000b8abe.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VTBCX2_l02n01_d0_4_fb.351000000b8abe.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3870560
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA10550.015.3387834
CTAGACT16800.014.17061
TCTAGAC17950.013.4939127
GGGGGAT4001.9488034E-613.0936341
CCCGGTT5653.965397E-912.6406771
CTGGGTT7205.456968E-1212.5645981
GTCATAA15750.011.4804965
CGGGGTT11050.011.2031111
CTCGGAT25200.011.1475381
CATTCTA24000.011.1055794
CTCGGTT14700.011.0125811
CTAGGAT7853.765308E-1010.917681
CTCCGAT15750.010.8830211
CTCAACC13300.010.7398231
CTGGGAT17750.010.7297391
CTCGCAT20350.010.5287221
GGGGTTA8601.6552804E-1010.515252
CTCGAAT34050.010.4874931
GGGGAAT10500.010.4295621
CTACGAT22000.010.3883381
Produced by FastQC (version 0.11.2)