FastQCFastQC Report
Thu 11 Jan 2018
H57VTBCX2_l02n01_d0_4_br.351000000b8a95.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VTBCX2_l02n01_d0_4_br.351000000b8a95.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6663183
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAT55850.019.558358
TCTCTTA64450.017.6854387
TCTAGAC33300.015.6883037
CTAGACT34700.014.7816128
ATTCTAG41050.013.1001695
CTTTTCT133900.012.51800251
GGTCATA19600.012.3856964
CGGGGAT22750.012.1400231
CGGGTAT16650.012.0117971
TTCTAGA46850.011.9668336
CATTCTA48900.011.4862814
TCTTATT97050.011.2553729
CTGGGAT23000.011.1799251
TAGACTT47100.010.8900629
CTCGGTT33750.010.5818211
CTCAGGT18600.010.4964841
CTCGGAT36450.010.4511811
CGGGAAT33700.010.314921
CTAGGAT11300.010.1135991
CTCAGAT26850.010.108891
Produced by FastQC (version 0.11.2)