FastQCFastQC Report
Thu 11 Jan 2018
H57VTBCX2_l01n01_hy_mut_bra7.351000000b8b2a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VTBCX2_l01n01_hy_mut_bra7.351000000b8b2a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4536056
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT702031.5476660781965654TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC90450.038.48797646-47
GAGCACA182200.038.369139
TATGCCG91350.038.31611348-49
ATGCCGT94300.036.96637348-49
AGAGCAC191250.036.5783278
TGCTTGA97800.035.91171660-61
GCCGTCT97900.035.8020750-51
CGTATGC99650.035.22060846-47
CCGTCTT99750.035.2104652-53
TGCCGTC100450.035.1059750-51
CTGCTTG100800.034.58377558-59
CCTTTAT101050.034.42828436-37
GCTTGAA101700.034.41782460-61
TCGTATG101850.034.36713444-45
CCAGTCA103700.033.93722526-27
CAGTCAC104550.033.29789726-27
AAGAGCA213600.032.7771647
AGTCACA107650.032.75922428-29
CTCGTAT107750.032.66162544-45
CTCCAGT108450.032.51560224-25
Produced by FastQC (version 0.11.2)