FastQCFastQC Report
Thu 11 Jan 2018
H57VTBCX2_l01n01_hy_mut_bra6.351000000b8b00.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VTBCX2_l01n01_hy_mut_bra6.351000000b8b00.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5262226
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT546301.0381538155145749TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC72750.036.98303246-47
TATGCCG74300.036.2431348-49
ATGCCGT76300.035.2308748-49
GCCGTCT79300.033.95842450-51
CCAGTCA82450.033.09259426-27
GAGCACA162950.033.0805669
TGCTTGA82550.033.05344860-61
CCGTCTT82250.032.8564252-53
TGCCGTC82600.032.8029850-51
CACACTG82550.032.50625630-31
CTGCTTG83550.032.23118658-59
ACACTGA84650.032.06452632-33
CAGTCAC84500.031.86822126-27
CACTGAT84750.031.74649432-33
CGTATGC85800.031.66243746-47
AGAGCAC171450.031.495938
GCTTGAA85900.031.46030660-61
TCCAGTC86600.031.15027624-25
TCGTATG87650.030.69612744-45
CTCCAGT88600.030.58111824-25
Produced by FastQC (version 0.11.2)