FastQCFastQC Report
Thu 11 Jan 2018
H57VTBCX2_l01n01_hy_con_bra5.351000000b8ae5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VTBCX2_l01n01_hy_con_bra5.351000000b8ae5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4098779
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT281390.6865215226290561TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC42750.032.4950146-47
TATGCCG44950.031.01025448-49
ATGCCGT46850.029.60057648-49
GCCGTCT47850.028.98214350-51
TGCTTGA48150.028.94934760-61
TGCCGTC49600.028.48622150-51
CTGCTTG49250.028.15811358-59
CCGTCTT49900.028.12477952-53
CCAGTCA50550.027.66985926-27
CAGTCAC51150.027.11315226-27
CGTATGC51700.027.0993246-47
GCTTGAA51900.026.90338360-61
CGAGTGG51900.026.53767432-33
GAGTGGA52450.026.39489734-35
AGTCACG53100.026.2963628-29
AGTGGAT53050.026.18589434-35
TCGTATG54200.025.84935244-45
ACTCCAG53950.025.7930722-23
GTGGATA54700.025.3977136-37
TCCAGTC56000.025.06103524-25
Produced by FastQC (version 0.11.2)