FastQCFastQC Report
Thu 11 Jan 2018
H57VTBCX2_l01n01_d10_w5_ov.351000000b8a6e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VTBCX2_l01n01_d10_w5_ov.351000000b8a6e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1522999
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT23760.1560079816204738TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGTT2702.2271706E-819.406331
CTCTTAT10250.013.9003838
TATGCCG8600.012.97773248-49
TCTCTTA11300.012.6099967
GTATGCC8500.012.5716746-47
ATTCTAG6501.9281288E-1012.4412555
CTAGACT5051.6449667E-712.2259148
CTGGCCT3551.2129969E-412.0761551
GGGAATC5604.7941285E-811.8935482
GGGGACG4456.3842435E-611.7746281
ATGTCAG9200.011.61512934-35
TCTAGAC4951.7105576E-611.5145827
ATGCCGT10000.011.398313548-49
CCGGGAT4204.584487E-511.3413621
GGGGATT3400.001155541911.1939272
CGTATGC10400.011.18824946-47
CGGGAAT7702.6557245E-1011.1351561
CTCGGAT8156.002665E-1111.1047941
CCAGTCA9900.011.03372126-27
CTCAGGT4751.3183029E-511.0309671
Produced by FastQC (version 0.11.2)