FastQCFastQC Report
Thu 11 Jan 2018
H57VTBCX2_l01n01_d10_w5_fb.351000000b8a45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VTBCX2_l01n01_d10_w5_fb.351000000b8a45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2615491
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT49370.18875996896949748TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAT2651.3971556E-414.3809461
TTCCGTA16800.013.71208636-37
GTTCCGT16000.013.65397434-35
CCGTATC16600.013.5927238-39
TATGCCG17800.013.47387948-49
TCACAGT18050.013.28802130-31
TCCGTAT17050.013.232450536-37
ATGCCGT17000.013.13016548-49
GTATGCC18100.012.59458646-47
GCCGTCT17550.012.58349350-51
CTAGACT9900.012.5106971
CGTATGC18700.012.44444946-47
GTCACAG18250.012.10141728-29
CCGTCTT19350.012.026648552-53
CTAGGAC3257.835174E-411.7260021
CTGGAAT16000.011.611491
GGGGTAT3701.7831996E-411.587351
AGTCACA19800.011.39395728-29
ACAGTTC21050.011.393805532-33
GGGTAGT3350.001010070911.3759731
Produced by FastQC (version 0.11.2)