FastQCFastQC Report
Thu 11 Jan 2018
H57VTBCX2_l01n01_d10_w5_br.351000000b8a2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VTBCX2_l01n01_d10_w5_br.351000000b8a2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2801743
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT50670.18085170552759477TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC15800.018.63634946-47
TATGCCG17050.016.99149748-49
TCTAGAC12400.016.8543287
CTAGACT11450.016.5913128
CTCTTAT19550.015.5474598
AGTCAAC19000.015.49773334-35
CCAGTCA19800.014.63208926-27
ATTCTAG15050.014.222795
ATGCCGT21150.014.03446648-49
CGTATGC21200.014.00136546-47
CCGTCTT21450.013.72747452-53
GTCACAG21500.013.69603828-29
CAGTCAC21500.013.69603826-27
TCACAGT22000.013.49270530-31
TCGTATG22050.013.35393744-45
CACAGTC21950.013.19887830-31
ACTCCAG22150.013.07946822-23
TCTCTTA24750.013.0501117
GAGCACA46250.012.9385339
GCCGTCT22650.012.895349550-51
Produced by FastQC (version 0.11.2)