FastQCFastQC Report
Thu 11 Jan 2018
H57VTBCX2_l01n01_d10_w4_ov.351000000b8a01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VTBCX2_l01n01_d10_w4_ov.351000000b8a01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1873290
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC108120.57716637573467TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC20050.027.59537144-45
GAGCACA39350.027.5178669
AGAGCAC40200.027.1723048
ATGCCGT21500.025.18204946-47
CGTATGC21950.024.88215444-45
TATGCCG22000.024.6097346-47
TCGTATG22600.024.58877442-43
CCTTGTA22250.024.54699132-33
CCAGTCA23250.023.0835926-27
AGTCACC23950.022.6072228-29
GTCACCT23950.022.6072228-29
CAGTCAC23650.022.59275826-27
AAGAGCA48700.022.4338957
TGCTTGA24050.022.41328258-59
GTAATCT24400.022.38463636-37
ACCTTGT24150.022.3207732-33
GCCGTCT24700.022.11187448-49
CTCGTAT24750.022.06897242-43
GCTTGAA25000.021.94151558-59
CCGTCTT24950.021.79542750-51
Produced by FastQC (version 0.11.2)