FastQCFastQC Report
Thu 11 Jan 2018
H57VTBCX2_l01n01_d10_w4_fb.351000000b89e6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VTBCX2_l01n01_d10_w4_fb.351000000b89e6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2123128
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC111420.5247917224020408TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC23700.023.84757444-45
TCGTATG24700.023.26830342-43
GTATGCC24600.023.07163644-45
TATGCCG25500.022.16421346-47
ATGCCGT25550.021.84200946-47
GAGCACA52350.021.4106929
ACGGCTA26250.021.07886932-33
AGAGCAC52800.020.8684148
TGCTTGA26550.020.84044558-59
CCGTCTT26900.020.65732650-51
GCCGTCT28100.020.28197748-49
GCTACAT29300.019.45154434-35
CCAGTCA28950.019.4417726-27
GCTTGAA29850.019.01381158-59
ACTCCAG29600.019.01349622-23
CTAGACT7750.018.9974528
CAGTCAC29800.018.88722426-27
CACGGCT29600.018.69435530-31
CTTGAAA31100.018.55502360-61
TCTAGAC8450.018.5504287
Produced by FastQC (version 0.11.2)