FastQCFastQC Report
Thu 11 Jan 2018
H57VTBCX2_l01n01_d10_w4_br.351000000b89d9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VTBCX2_l01n01_d10_w4_br.351000000b89d9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1645763
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC75100.45632329806904154TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGCT15050.025.40339932-33
CACTAGC14850.025.26618430-31
CTAGCTT15000.025.17145332-33
TAGCTTA15450.024.89902734-35
TCACTAG15750.024.42549930-31
GTATGCC16100.024.18803644-45
TATGCCG16350.023.9634246-47
AGCTTAT17100.022.63535734-35
TCGTATG17750.021.94357142-43
GCTTATC18000.021.76908936-37
GTCACTA17700.021.33206428-29
TGCTTGA18700.021.20593858-59
CCAGTCA18650.021.1361126-27
AGTCACT18950.020.80213428-29
CGTATGC18750.020.7694644-45
GAGCACA37450.020.6703789
AGAGCAC38500.020.2299948
ATGCCGT19350.020.0027346-47
CTTATCT20100.019.73100736-37
GCTTGAA19900.019.56921458-59
Produced by FastQC (version 0.11.2)