FastQCFastQC Report
Thu 11 Jan 2018
H57VTBCX2_l01n01_d10_w3_ov.351000000b89bf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VTBCX2_l01n01_d10_w3_ov.351000000b89bf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1341147
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC173341.2924757688754478TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA45450.035.4214369
GTATGCC23200.034.3917344-45
AGAGCAC47500.033.7927448
TATGCCG24300.033.5177246-47
CGTATGC24750.032.81358344-45
ATGCCGT25050.032.04022246-47
TCGTATG25250.031.88404742-43
CCAGTCA26200.031.36127726-27
GCCGTCT25700.031.32109648-49
CTCGTAT26550.030.68066242-43
AGTCACG26850.030.60320728-29
TGCTTGA26800.030.39219558-59
CAGTCAC26750.030.18381126-27
GCTTGAA26600.030.17434358-59
CAGATCT26600.029.99467836-37
CCGTCTT27200.029.94413650-51
GATCAGA27600.029.50961334-35
AAGAGCA54600.029.4931247
TCAGATC27400.029.46557636-37
ATCAGAT27250.029.365834-35
Produced by FastQC (version 0.11.2)