FastQCFastQC Report
Thu 11 Jan 2018
H57VTBCX2_l01n01_d10_w3_fb.351000000b8996.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VTBCX2_l01n01_d10_w3_fb.351000000b8996.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2009591
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC220571.097586523824997TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG33750.031.59245946-47
GTATGCC33200.031.25749644-45
TGCTTGA35550.029.92603958-59
CGTATGC35800.029.7833944-45
CCGTCTT36100.029.40432250-51
TCGTATG35450.029.27505142-43
ATGCCGT35350.029.15487346-47
AGTCACA38850.027.751528-29
GCCGTCT37950.027.40773648-49
TCTGCTT39250.027.28649356-57
CTCGTAT39050.027.1843542-43
GCTTGAA38800.027.17453458-59
CTGCTTG39150.026.68896756-57
TCACACT40600.026.49682230-31
CTTGAAA40400.026.27465260-61
GAGCACA80600.026.1041479
AGAGCAC81150.025.7516468
CCAGTCA41600.025.74570826-27
CACACTT40700.025.55649830-31
GTCACAC40950.025.4584728-29
Produced by FastQC (version 0.11.2)