FastQCFastQC Report
Thu 11 Jan 2018
H57VTBCX2_l01n01_d10_w3_br.351000000b896f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VTBCX2_l01n01_d10_w3_br.351000000b896f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1378084
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC168011.2191564520014746TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC25750.031.6306444-45
TATGCCG26150.031.32842346-47
TGCTTGA26900.030.63150258-59
ATGCCGT27100.030.31781846-47
CGTATGC27400.029.89920644-45
GCTTGAA27850.029.50135858-59
GCCGTCT27800.029.2127548-49
CCGTCTT28000.029.173750-51
TCGTATG28250.029.08786242-43
CTGCTTG28400.029.01364156-57
CTTGAAA29450.027.979260-61
CAGATCA29250.027.92747134-35
CCAGTCA29800.027.33581426-27
CTCGTAT30200.027.28831342-43
AGATCAT30300.027.27316934-35
AGTCACC30050.027.10839528-29
GAGCACA61250.026.8281759
ACCAGAT30300.026.80294232-33
TGCCGTC30800.026.52154748-49
CAGTCAC30850.026.48240526-27
Produced by FastQC (version 0.11.2)