FastQCFastQC Report
Thu 11 Jan 2018
H57VTBCX2_l01n01_d10_w2_ov.351000000b8946.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VTBCX2_l01n01_d10_w2_ov.351000000b8946.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2362754
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC90190.3817155742832305TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC19100.023.37288944-45
TATGCCG20650.021.15853746-47
CGTATGC21750.020.52515444-45
TCGTATG22200.019.7898942-43
GCCGTCT23200.019.5493948-49
CACGCCA22700.019.24855830-31
ATGCCGT23300.019.15974846-47
GCTTGAA23950.019.13549258-59
TCACGCC23550.018.65464830-31
CCAGTCA23100.018.50404726-27
GAGCACA48400.018.3490739
CCGTCTT23950.018.342750-51
CTCGTAT24450.018.16298942-43
ACGCCAA23950.018.14420932-33
GCCAATA24650.018.0142934-35
CAGTCAC25050.017.727226-27
GTCACGC25250.017.68083228-29
AGAGCAC50900.017.3545368
CCAATAT26100.017.28643834-35
CGCCAAT25950.017.20334632-33
Produced by FastQC (version 0.11.2)