FastQCFastQC Report
Thu 11 Jan 2018
H57VTBCX2_l01n01_d10_w2_fb.351000000b892c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VTBCX2_l01n01_d10_w2_fb.351000000b892c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7445923
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC251420.33766129464406225TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC63850.018.93002544-45
TATGCCG65500.018.70693846-47
CGTATGC72600.017.26997244-45
ATGCCGT74000.016.49398846-47
CCGTCTT75900.016.48809250-51
TCGTATG75050.016.20168742-43
AGTCACA77650.015.78089728-29
GCCGTCT78450.015.55838448-49
CACAGTG78850.015.47981932-33
TGCTTGA81150.015.39197858-59
GTCACAC79550.015.22486528-29
ACAGTGA83250.014.9181934-35
ACACAGT83750.014.85767832-33
AGTGATC83750.014.68814536-37
CTGCTTG85500.014.58149456-57
CCAGTCA84150.014.4490526-27
GTGATCT86200.014.24312536-37
CAGTGAT87100.014.0679334-35
CACACAG87250.014.04454730-31
CAGTCAC87350.014.00128126-27
Produced by FastQC (version 0.11.2)