FastQCFastQC Report
Thu 11 Jan 2018
H57VTBCX2_l01n01_d10_w2_br.351000000b8902.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VTBCX2_l01n01_d10_w2_br.351000000b8902.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5569899
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC189770.34070635751204825TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC48850.018.71514544-45
TATGCCG52800.017.76471546-47
ACTGACC53850.017.33044432-33
CGTATGC57850.016.33716644-45
CTGACCA56250.016.12663332-33
TGCTTGA59600.015.77778158-59
CACTGAC58350.015.66910430-31
CCAATCT57800.015.61263336-37
ATGCCGT59150.015.53643146-47
TCGTATG59500.015.52646342-43
TGACCAA59350.015.48421534-35
CCAGTCA61450.015.26509526-27
ACCAATC59900.015.22386136-37
GACCAAT59800.015.20885734-35
AGTCACT62000.015.12967928-29
GCCGTCT62200.015.00359348-49
CTGCTTG65250.014.70312256-57
CCGTCTT64300.014.58770750-51
GCTTGAA64500.014.50552758-59
TCACTGA65200.014.35069430-31
Produced by FastQC (version 0.11.2)