FastQCFastQC Report
Thu 11 Jan 2018
H57VTBCX2_l01n01_d10_w1_ov.351000000b88f4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VTBCX2_l01n01_d10_w1_ov.351000000b88f4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6299966
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC179700.28523963462659957TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGG38650.023.4086632-33
CTTAGGC40700.022.40463632-33
TAGGCAT42500.021.39977634-35
CACTTAG42550.021.09634230-31
TTAGGCA45200.020.38415134-35
GTATGCC46850.019.5141144-45
TATGCCG50800.018.27724346-47
AGGCATC51200.018.04255336-37
TCACTTA50950.017.89789430-31
CGTATGC55200.016.82036244-45
GGCATCT55650.016.72781636-37
ATGCCGT55600.016.40038746-47
TCGTATG57000.016.3322842-43
AGTCACT57750.016.20164528-29
CCAGTCA58750.015.8450326-27
CCGTCTT59550.015.51204150-51
GCCGTCT59500.015.44513248-49
TGCTTGA60050.015.30379358-59
CTCGTAT63350.014.99508542-43
CAGTCAC63750.014.60228326-27
Produced by FastQC (version 0.11.2)