FastQCFastQC Report
Thu 11 Jan 2018
H57VTBCX2_l01n01_d10_w1_fb.351000000b88da.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VTBCX2_l01n01_d10_w1_fb.351000000b88da.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4176966
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC108530.2598297424494238TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTAGG7600.019.3995045
GTATGCC29850.017.02364244-45
TATGCCG31650.016.50562346-47
CGTATGC34500.014.93563344-45
ATGCCGT36000.014.31331446-47
CCGTCTT36300.014.19536350-51
TCGTATG37250.013.89924542-43
TCTAGGA11100.013.2648926
GCCGTCT40300.013.08069848-49
CTAGGAT12600.012.8526211
AGTCACC41500.012.47418528-29
ATTCTAG23050.012.3800625
CCAGTCA41650.012.08717126-27
GATCTAG12200.012.0853714
CAGTCAC42550.012.05474326-27
TGCTTGA43200.012.03798458-59
CTAGACT18450.011.61714651
CTCGTAT46000.011.41025742-43
CTAGAAT22350.011.2948821
TCTAGAC20250.011.2583837
Produced by FastQC (version 0.11.2)