FastQCFastQC Report
Thu 11 Jan 2018
H57VTBCX2_l01n01_d10_w1_br.351000000b88b0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VTBCX2_l01n01_d10_w1_br.351000000b88b0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3986561
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC125140.3139046411179962TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC32900.016.88929444-45
TATGCCG34650.016.85867346-47
CTCTTAT28500.015.8307058
TGCTTGA38400.015.33599758-59
TCTCTTA32750.014.648267
CGTATGC39850.014.36085344-45
ATGCCGT39250.014.21738546-47
GCTTGAA40150.014.19440658-59
GCCGTCT40200.014.117681548-49
CCGTCTT41000.014.0162350-51
TCTAGAC12300.013.90187557
TCGTATG40550.013.58727242-43
CCAGTCA42750.013.4977626-27
CTAGACT12050.013.4002668
CTGCTTG43600.013.3985156-57
CAGTCAC44200.012.7863426-27
TGCCGTC45650.012.48423748-49
ACTCCAG46250.012.37362122-23
AGTCACA46450.012.32034528-29
GTCACAT46350.012.19323128-29
Produced by FastQC (version 0.11.2)