FastQCFastQC Report
Thu 11 Jan 2018
H57VTBCX2_l01n01_d0_3_ov.351000000b8acb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VTBCX2_l01n01_d0_3_ov.351000000b8acb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3917855
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAT31900.019.5032088
TCTCTTA37050.017.691797
TCTAGAC17050.014.4863797
CTAGACT16250.013.7363058
CTGGGAT12950.013.6090721
CGGGGAT13050.012.7747991
CTGGAAT22700.012.1702281
CTGGGTT7451.0913936E-1112.1476951
CTTTTCT77950.011.9766821
CTAGAAT19400.011.0486011
TCTTATT57750.010.9376779
ATTCTAG22200.010.9262065
CTCAGAT17100.010.8633821
ATTTCCC29350.010.84170812-13
CGGGGTT6603.9315637E-810.8253981
CTCGGTT15450.010.7903641
CGGGAAT17800.010.7037641
GGGGGAT5354.207528E-610.6837571
CCGGGTT8106.4574124E-1010.5848341
CGGGACT11350.010.4915751
Produced by FastQC (version 0.11.2)