FastQCFastQC Report
Thu 11 Jan 2018
H57VTBCX2_l01n01_d0_3_fb.351000000b8aa2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VTBCX2_l01n01_d0_3_fb.351000000b8aa2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2423013
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT29700.1225746622077554No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC25590.10561230996284378No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT24880.1026820739302678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACT16250.019.0555211
CCCTGAT8900.017.6638261
TCTAGAC15700.016.33737
CGGGTCT6550.015.2735081
CATTCTA19950.015.0220664
TCTCTTA17250.014.869317
TAGACTT19900.014.5823232
GGTCATA8900.014.4313114
ATTCTAG20950.013.623835
CGGGGAT7900.013.2665011
TAGAATA15950.013.1232662
CTCTTAT19000.012.9976318
TTCTAGA21750.012.8848376
TAGCCTA5100.012.10613656-57
CTTTCGT28150.012.015471
GACCCTA6051.1090378E-811.7941835
CGGGGCT3651.570511E-411.7465531
TCTAGGA4456.5931654E-611.7413727
GAACCCA8105.456968E-1211.72659
CCCATAA15450.011.680969
Produced by FastQC (version 0.11.2)