FastQCFastQC Report
Thu 11 Jan 2018
H57VTBCX2_l01n01_d0_3_br.351000000b8a88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57VTBCX2_l01n01_d0_3_br.351000000b8a88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3513646
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAT28000.015.9437898
TCTCTTA30650.015.1870347
CTAGACT22650.015.139281
TCTAGAC20450.014.4004397
ATTCTAG26300.012.6588685
CCCCACT4152.936009E-612.6236661
GGTCATA11200.012.3149474
CTGGAAT16250.012.0163181
TTCTAGA28050.011.6840536
TAGACTT27450.011.5918879
TTCGTAC33750.011.5556033
CGGGAAT17600.011.0946121
CGTACAA36800.010.9855975
GTCATAA17000.010.9110935
CTCCTAT10250.010.6867321
CTGGGTT5354.213576E-610.6823891
CCCGGAT8953.092282E-1110.6426031
GACTTCA32250.010.6183084
CTCGGAT17650.010.5235151
CATTCTA30300.010.5168274
Produced by FastQC (version 0.11.2)