FastQCFastQC Report
Thu 21 Mar 2019
H573CBGXB_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH573CBGXB_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences373423568
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCGG107613500.069.809366
TGTCGGT107816800.069.803347
ACCGGTG1836050.069.7966162
TCGGTCT109909300.069.773079
GTCGGTC109899200.069.765898
CGGTCTC110004700.069.71323410
GGTCTCG110010750.069.7020911
ACGCGTG1530300.069.632482
GTCTCGT110104400.069.6221312
TCACGTG1616300.069.620892
CTCGTAG108729350.069.61111514
ACGAGGT93114350.069.6067210
GTAGGGG1370250.069.6037517
GGTCTCT93147600.069.5940914
AGGTCTC93370400.069.5060413
CGAGGTC93240100.069.5037511
CACGAGG93166650.069.4939659
GAGGTCT93293000.069.4860512
CCACGAG93156450.069.484098
TCGTAGG27939200.069.4092715
Produced by FastQC (version 0.11.5)