FastQCFastQC Report
Sat 30 Oct 2021
H572WAFX3_n01_9251chp4579.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH572WAFX3_n01_9251chp4579.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1962370
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT675073.4400750113383305TruSeq Adapter, Index 25 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTTT42790.21805266081320038TruSeq Adapter, Index 25 (97% over 44bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT42660.2173901965480516TruSeq Adapter, Index 25 (97% over 41bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT42420.2161671855970077TruSeq Adapter, Index 25 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAA28650.14599693228086447TruSeq Adapter, Index 25 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCGCGTAT22930.11684850461431841TruSeq Adapter, Index 25 (97% over 45bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)