FastQCFastQC Report
Mon 29 May 2023
H55TVDSX7_n01_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55TVDSX7_n01_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2858809924
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCATCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGTTGAC44842280.15685645842888854No Hit
TGTTGGAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGTTGAC38931850.13618201641586297No Hit
TGGTACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGTTGAC37969730.13281656007011958No Hit
TGCAGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGTTGAC34104550.11929631877127904No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)