FastQCFastQC Report
Mon 29 May 2023
H55TVDSX7_n01_A48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55TVDSX7_n01_A48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22722
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCTAAGGATGCTGAGGTCCTGCAAAGCCTGGGGCTTCTCAGTTTCACA9984.392218994806795No Hit
TATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGTCAAAGT7873.463603556024998No Hit
CCGTGACCCCCAGGTGACCTTCGACGCCACCAAGCACCTGAGCGAGGCCT7033.093917788927031No Hit
GTACTGCGGCAAGTACTATTGACCCAGCGATGGGGGCTTCGACATGGGCT6742.966288178857495No Hit
GACTTACCCATCAACAACCGCTATGTATTTCGTACATTACTGCCAGCCAC6702.948684094709973No Hit
TTCAAAAACTCAGCATTGGGTGGCTCTGTGTCCTTTGGCAGCAAGGCCTG6152.7066279376815423No Hit
GTCCTGGAATGGGATAAATCCTGTGTGACCACTAGGTGGCGGTAGAGTTT5202.2885309391778894No Hit
CCTCCAGCACGAGCAGCTAAGTCCCGCCCCTCAGGTCGTGTCAACGGCCA5142.262124812956606No Hit
GATATTGCTAGGGTGGCGCTTCCAATTAGGTGCATGAGTAGGTGGCCTGC4832.1256931608133085No Hit
ATCCCTAGAAGTGGAAGTGCTGGGCCAACGCCTGTCTCTTATACACATCT4712.0728809083707422No Hit
GCTCAGGCTGATCTCAAACTCCTGGCTTCAAGTGATCCTCCTGCCTCAGC4702.0684798873338615No Hit
ACACAGGGCGCTGGTGGGCGGGAAGAGTTGAGAACATTGACCCTGTCTCT4582.0156676348912947No Hit
GATCTACACTACCACTGAGGCAGGAGAATAGGGTCTGGAGGCAGGGAAGC4531.9936625297068922No Hit
CCCCGCGCCCGTCCGTCTGCCAGGCGCGGCCTACCATTGGCTGCGCGCCA3811.6767890150514921No Hit
GCGTGGATACGTTTGCGGCCTCTGCCCGCGGGCCCCGGCGCTTCGGCCTC3751.6503828888302086No Hit
ATCTTGTCCAGGCTGGTCTTGAACTCCTGACCTCGTGATCCACCCGCCTC3591.5799665522401196No Hit
TCCCTCAGAGCCTAGCGAGGGGGCGTGGCCCAGCCAGGAGGGCTGGAGAG3481.5315553208344335No Hit
AACCTGGACACAAAGACCGTCAGCTCTGTTGGCTCTGGAGAACTGCCTAA3421.5051491946131503No Hit
GCAATAAACGAAAGTTTGACTAAGTTATACCTCTTAGGGTTGGTAAATTT3131.3775195845436141No Hit
CTGATGGCCTGGAACAGCACCCACACCCCCAGGTGAGTATCTGACGGCCT3051.3423114162485696No Hit
CCACCTGCGACCGCTGGCCGCCTCCAGGCCTGGCTGGGAGCACATTACGT2981.3115042689904057No Hit
CCTATGACACCCGGAACATTTCAGCTTACTCTGGGCAAGAAGTGGCTACG2971.3071032479535252No Hit
TGTTAGGACCCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGA2841.2498899744740781No Hit
CCACCTACCTCCCTCGCCAAAGCCCATAAAAATAAAAAATTATAACAAAC2811.2366869113634362No Hit
GTCCTAGAAATAAGGGGGTTTAAGCTCCTATTATTTACTCTATCAAAGTA2801.2322858903265557No Hit
TCCAAGAGTGGGGGGCGGATGGTGATTGGCTGAGCCCGGTTGCCGGAGCC2701.1882756799577503No Hit
TCCCCCAACCCCCACCGGGGTAGGGGGTTGGGGGAGGTGGGAGGAAAACA2501.1002552592201391No Hit
ACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTT2491.0958542381832586No Hit
GGAGGAGGAGGAGGAGGGGAAAAGATCCTGTGGAACAAACACAGCAATGC2451.0782501540357363No Hit
CCTTAAATCCCAGCATCTGGGAGGCATTAGGCAGGCGGGTTTGTAAGCCC2421.0650470909250946No Hit
GGAGATTGATGCTTCTGTTTTTTGTTGCCGCTGCTGCCCTCGCGCTGGGA2421.0650470909250946No Hit
TCCTGCTGGGACCCGGCGGCACGCCCCTGCCCCCGCCCCGGCCGAGCCTG2361.0386409647038113No Hit
GGACAACTTCTTCAACAAATGATGCTGGGGAAACTGGATATCCATATGCA2311.0166358595194085No Hit
GACCACAGTTTGAGTCCAAGAGTGCCACTGGCCATCCAGATTCACAATGG2240.9858287122612447No Hit
ATCTTGGGCTGCTCAAACTCGCGTCGCCGGAGCTGGAGAGGCTGATCATC2000.8802042073761113No Hit
TCTCTGAGTCACCCTGCACTCAGGCATTTTATCTGTGCCTGTGGTGTGCT1940.8537980811548279No Hit
GAGTCAGCTTTGCCGGGAGCATAAAACATTTAATGGCCCATAAAACTGAC1870.822990933896664No Hit
GTGTAAGAGCGAGGTCTGACTCCATTTCCCAGGCTGCCCCGGGACAGATC1700.7481735762696945No Hit
ACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGAGAGGT1640.7217674500484111No Hit
GCCCCACACGGAGAGGGCAGCCGGAGAGGGGCGCGGTGCGGGAGGCGGGG1610.7085643869377696No Hit
GAGGATTGTAAGGCAGAGTACAAAAGAGGGCGTATTAATGACAATACATT1500.6601531555320834No Hit
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC1320.5809347768682335No Hit
CTACCATATCTGCTGAAGGTAGGGTACCCACATTGCTGCCCCATGACTGT1290.5677317137575918No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCCTTATGAGGCGGAGACG1220.5369245664994279No Hit
TCCTAATGCTGCAACCTTTGACACAGTTCCTCAGACTGTGGTGACCCCGA1190.5237215033887862No Hit
GTATGGGGGTAATTATGGTGGGCCATACGGTAGTATTTAGTTGGGGCATT1180.5193204823519056No Hit
GGAAAGGCCTGTAAAGTCAGGCAGGCACACTGCCCAGAGGCTGTCTCTTA1080.47531027198310005No Hit
GTTTGTACCTTTGCCAGAAAATTATTTTCCAATGACTATTACTCAACTCT870.38288883020860837No Hit
GATGCCAACTCCGCCACCGAGGTGCTAGGTCCCCTACCCAGCACCTCGGT840.36968576709796674No Hit
TCTCCCTACAGGCCACTTTTCGGTAGGTGTAGAAAAAAATCATGTAGGAA810.35648270398732507No Hit
GGGCCATGAAGAGTATCAAGTGAGTGTGAGGAAATCAGTATATTGAGGAA710.31247249361851953No Hit
CCTCATTGGAGGATTCTAGATAGAGTTCCAGCCCTGTCCACACTCCCAGC700.3080714725816389No Hit
CCTCTATCCAAGAAGGGCACCAGGTTCTTTACTTTGCTTTATTGTTTGTG650.2860663673972362No Hit
GGCGTGGTCCGAGGGCTCCATGCTGCCGTACCCAGGCCCTGTCTCTTATA520.2288530939177889No Hit
AGTGACTGCCTTGAACAGTAACAAAGGTGAGCAATGGGTATAGGATAGGT500.22005105184402782No Hit
AGGCTCGCTCATAGGCCCGGAACACCACAGCCCGCCCAGACTTGGCTGGC490.21565003080714723No Hit
GGCTTCAGCTCTGGTTTATGCAATAAATGCAGATGACAAGGATGCATTGA280.12322858903265559No Hit
CGTGTTGTGCGGGGGGGCGGGGCGGGGCGATGGGGGCGGGCCCCGGCTAG250.11002552592201391No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)