FastQCFastQC Report
Mon 29 May 2023
H55TVDSX7_n01_A33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55TVDSX7_n01_A33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences881475
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCCAGGGGATCCACCCCTCACCCGTGCTGAGAGTGTGGGTCTACGGG12910.14645906009813095No Hit
GGCGGTGGGGCCGGGGGCCCGGCCCAGGGCGGGAAATGGGTGGGGAGGGG11560.13114382143566183No Hit
ATTTATGTGCTCCGGGTCCATCATCCACAACCTTAACAATGAACAAGATA10060.11412688958847385No Hit
ATCCTTTCCTGGGAAGTTGGTTTGCCGCCGCTGCCTGGATATTAGCACAG9920.11253864261606965No Hit
TCCCAGACTCCCTTTCGTTCCCGACATCACTTGCAGTTTCTAAGAGCATC9830.11151762670523838No Hit
TTCCTCTCCTGTCCAAGGCCACTGTGGAGAAACAAACTTGGGAGGGATGA9300.1055049774525653No Hit
TCCTTTCTCCAGCCTCTCGCGCCCAGTTGACTCCCGGCAACACCTCCCGC9220.1045974077540486No Hit
GGCCAGGGGATTAATTAGTACGGGAAGGGTATAACCAACATTTTCGGGGT9200.10437051532941945No Hit
GGGGCGGGCGGTGGACCCCCAGCTGGCAGAGCCTGGGGCGGGGGCCTGAG9100.10323605320627359No Hit
GCCGAGGAGACTAGGAGGGGGAGGAGAGGGGATCTCGCGAAAGGAAAGAG8830.10017300547377973No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)