##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H55MKBCXX l02n02 7_m30a.3420000000ed55.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14275336 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.0780053092971 35.0 35.0 35.0 32.0 35.0 2 34.040427910068104 35.0 35.0 35.0 32.0 35.0 3 34.0301361733272 35.0 35.0 35.0 32.0 35.0 4 34.021751431980306 35.0 35.0 35.0 32.0 35.0 5 33.995532504453834 35.0 35.0 35.0 32.0 35.0 6 38.67856602464558 40.0 39.0 40.0 36.0 40.0 7 38.762029909488646 40.0 39.0 40.0 37.0 40.0 8 38.729014154202744 40.0 39.0 40.0 37.0 40.0 9 38.76634826668879 40.0 39.0 40.0 37.0 40.0 10-14 38.744696741288614 40.0 39.0 40.0 36.8 40.0 15-19 38.705962745815576 40.0 39.0 40.0 36.6 40.0 20-24 38.718017411288955 40.0 39.0 40.0 36.4 40.0 25-29 38.701127286951426 40.0 39.0 40.0 36.6 40.0 30-34 38.67808823554135 40.0 39.0 40.0 36.0 40.0 35-39 38.645039962632055 40.0 39.0 40.0 36.0 40.0 40-44 38.5747283846769 40.0 39.0 40.0 36.0 40.0 45-49 38.48956020369678 40.0 39.0 40.0 36.0 40.0 50-54 38.46293949228235 40.0 39.0 40.0 36.0 40.0 55-59 38.440324234750065 40.0 39.0 40.0 36.0 40.0 60-64 38.444728502362395 40.0 39.0 40.0 36.0 40.0 65-69 38.38726756414 40.0 39.0 40.0 36.0 40.0 70-74 38.29193435446983 40.0 39.0 40.0 35.8 40.0 75-79 38.255159444233044 40.0 39.0 40.0 35.2 40.0 80-84 38.20986983423718 40.0 39.0 40.0 35.0 40.0 85-89 38.16023789562641 40.0 39.0 40.0 35.0 40.0 90-94 38.154402138065265 40.0 39.0 40.0 35.0 40.0 95-99 38.11086772318354 40.0 39.0 40.0 34.8 40.0 100-104 37.02025214677959 39.0 37.6 39.4 32.0 39.6 105-109 38.049352631699875 40.0 39.0 40.0 34.4 40.0 110-114 38.091625500093315 40.0 39.0 40.0 34.4 40.0 115-119 38.008915208720836 40.0 39.0 40.0 34.0 40.0 120-124 37.9150706505262 40.0 39.0 40.0 34.0 40.0 125-129 37.807965991133244 40.0 39.0 40.0 34.0 40.0 130-134 37.761165733682205 40.0 39.0 40.0 34.0 40.0 135-139 37.66681707526884 40.0 39.0 40.0 34.0 40.0 140-144 37.53664278024699 40.0 39.0 40.0 34.0 40.0 145-149 37.34090390586954 40.0 39.0 40.0 31.4 40.0 150-151 35.48945713081639 38.5 35.0 39.5 23.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.3551991947898898 2201 2 0.3417240790016365 2201 3 0.3184220565195446 2201 4 0.37684094282906955 2201 5 0.272215343649691 2201 6 0.49916115387114957 2201 7 0.45316291292099464 2201 8 0.5212745093181113 2201 9 0.4847901902278977 2201 10-14 0.4868314253456987 2201 15-19 0.48580018410964954 2201 20-24 0.46567845069373703 2201 25-29 0.4709055698043443 2201 30-34 0.4811142760877871 2201 35-39 0.4755455071310237 2201 40-44 0.4534347492030406 2201 45-49 0.4565336738227259 2201 50-54 0.4504437420042464 2201 55-59 0.46474643970148577 2201 60-64 0.43734058456022495 2201 65-69 0.437766542111369 2201 70-74 0.4265014406621006 2201 75-79 0.4226155918540684 2201 80-84 0.47945948783606696 2201 85-89 0.46130280591238915 2201 90-94 0.46046264047252095 2201 95-99 0.44023605818172484 2201 100-104 0.4032233948651722 2201 105-109 0.4623024637289035 2201 110-114 0.4616005022060037 2201 115-119 0.4650276037192569 2201 120-124 0.47800909979794426 2201 125-129 0.5143127108373449 2201 130-134 0.4646405130912967 2201 135-139 0.4326461011258047 2201 140-144 0.4700451313208234 2201 145-149 0.4505037906811893 2201 150-151 0.543153091496599 1101 1 0.3115150802556741 1101 2 0.3114497143041177 1101 3 0.32850537910635325 1101 4 0.3152165839822487 1101 5 0.32048127297913 1101 6 0.45727601742701296 1101 7 0.40441158347056216 1101 8 0.4922469544280901 1101 9 0.4403911343342699 1101 10-14 0.46002052167359864 1101 15-19 0.4677375307905791 1101 20-24 0.43910102829689635 1101 25-29 0.45411824529251277 1101 30-34 0.44181668822741216 1101 35-39 0.45497472266134054 1101 40-44 0.44556407935422015 1101 45-49 0.4611559908818066 1101 50-54 0.42196886981756165 1101 55-59 0.45602560158259564 1101 60-64 0.4264902259240202 1101 65-69 0.46276016222861216 1101 70-74 0.45636624790409996 1101 75-79 0.4508984481224161 1101 80-84 0.4580524591573294 1101 85-89 0.46589081384649944 1101 90-94 0.4761678836613399 1101 95-99 0.45720198410137414 1101 100-104 0.5113717119029744 1101 105-109 0.5001743840767432 1101 110-114 0.4556299906179717 1101 115-119 0.4902691076753882 1101 120-124 0.5072085655820331 1101 125-129 0.5305178644652031 1101 130-134 0.5239152627399122 1101 135-139 0.5800855231048629 1101 140-144 0.5554064102056486 1101 145-149 0.548139846494152 1101 150-151 0.6473742970367127 2202 1 0.24342752719409333 2202 2 0.21114193497410838 2202 3 0.2371017454605564 2202 4 0.18478597910091565 2202 5 0.2477399270519598 2202 6 0.36977547581309267 2202 7 0.3451440265276844 2202 8 0.41471541884642704 2202 9 0.4120084282697647 2202 10-14 0.37964745536102384 2202 15-19 0.38412149267490747 2202 20-24 0.384036640867194 2202 25-29 0.3902994358449092 2202 30-34 0.39750674084503146 2202 35-39 0.40311126776531836 2202 40-44 0.35623576622049313 2202 45-49 0.38739978397772745 2202 50-54 0.3569793145886848 2202 55-59 0.3572268078644427 2202 60-64 0.3951744681304703 2202 65-69 0.3926750634341829 2202 70-74 0.3760551327635966 2202 75-79 0.3528045793971586 2202 80-84 0.4030191808438133 2202 85-89 0.39936953878707726 2202 90-94 0.36801067468108073 2202 95-99 0.373117280152762 2202 100-104 0.4533575320406271 2202 105-109 0.41442693255723384 2202 110-114 0.35212843142473815 2202 115-119 0.3932209022079931 2202 120-124 0.3600746120227285 2202 125-129 0.4509390781141178 2202 130-134 0.4094427775980094 2202 135-139 0.41468365830174747 2202 140-144 0.43680659044919423 2202 145-149 0.4239025746301692 2202 150-151 0.593221398074526 1102 1 0.22393433028700116 1102 2 0.15175926774308834 1102 3 0.1729863745720266 1102 4 0.19337520750786297 1102 5 0.1660358873757346 1102 6 0.28351522313351296 1102 7 0.2767438179812629 1102 8 0.3720492238053055 1102 9 0.35073453781276953 1102 10-14 0.35476982329169005 1102 15-19 0.35439748265978466 1102 20-24 0.3404753826037705 1102 25-29 0.3279222481551045 1102 30-34 0.3448512674024897 1102 35-39 0.3641217331066784 1102 40-44 0.35019371086266204 1102 45-49 0.35773467186074015 1102 50-54 0.3402749594020307 1102 55-59 0.3392498271930222 1102 60-64 0.2970347164656175 1102 65-69 0.32136542556744985 1102 70-74 0.2789560309914805 1102 75-79 0.32515149632197193 1102 80-84 0.35346061457504874 1102 85-89 0.35086548775839077 1102 90-94 0.3104048645856139 1102 95-99 0.3180384861274632 1102 100-104 0.42118959965908687 1102 105-109 0.33977511226426316 1102 110-114 0.342366595793834 1102 115-119 0.3303518557641567 1102 120-124 0.3566024534777199 1102 125-129 0.39421213668690314 1102 130-134 0.34018728602156045 1102 135-139 0.3482406819982842 1102 140-144 0.3478671330807259 1102 145-149 0.40371218927155184 1102 150-151 0.620745153922492 2203 1 0.13667084764891513 2203 2 0.09229317665691639 2203 3 0.12421278960309934 2203 4 0.0978047554009791 2203 5 0.09918768163598912 2203 6 0.232366442059174 2203 7 0.24229323869585784 2203 8 0.29868482403551866 2203 9 0.2511557766234347 2203 10-14 0.2586069453500244 2203 15-19 0.2810221525858907 2203 20-24 0.284962076765801 2203 25-29 0.2729023612740349 2203 30-34 0.27370981810353356 2203 35-39 0.275212360485817 2203 40-44 0.22833779365186047 2203 45-49 0.2523525272062699 2203 50-54 0.20859638383554824 2203 55-59 0.2384272101206122 2203 60-64 0.24464880505262698 2203 65-69 0.22895379435897212 2203 70-74 0.2498153792309452 2203 75-79 0.2403871833831488 2203 80-84 0.2568720257967385 2203 85-89 0.254612112066674 2203 90-94 0.26305363536379645 2203 95-99 0.2297112135916919 2203 100-104 0.5389317140310013 2203 105-109 0.2765594924445196 2203 110-114 0.24361485663974491 2203 115-119 0.254175872207405 2203 120-124 0.20335970898852196 2203 125-129 0.30099061511440084 2203 130-134 0.2671903330349039 2203 135-139 0.23844036749980546 2203 140-144 0.27689381795411805 2203 145-149 0.3030414134824895 2203 150-151 0.6270997479974483 1103 1 0.07819080161408465 1103 2 0.06579765763784451 1103 3 0.11012582847776997 1103 4 0.08523666873553992 1103 5 0.10225266258991894 1103 6 0.09739975256158573 1103 7 0.14356376678036753 1103 8 0.2328273031662107 1103 9 0.17067291439959575 1103 10-14 0.15975690047780233 1103 15-19 0.18041622901592547 1103 20-24 0.15611836303600768 1103 25-29 0.12741987279740385 1103 30-34 0.1457140142244171 1103 35-39 0.17838635793711433 1103 40-44 0.16703703340340326 1103 45-49 0.14950639705411106 1103 50-54 0.11072996154090475 1103 55-59 0.11388521965876919 1103 60-64 0.140810900572788 1103 65-69 0.14074710568258553 1103 70-74 0.14782629392799151 1103 75-79 0.1273046885746254 1103 80-84 0.13921869092631312 1103 85-89 0.16648893558834743 1103 90-94 0.1477264125342046 1103 95-99 0.1487248585986194 1103 100-104 0.2666373956955326 1103 105-109 0.1628206595525441 1103 110-114 0.2156467472787469 1103 115-119 0.1581519672754581 1103 120-124 0.16545335101448444 1103 125-129 0.2453096179869192 1103 130-134 0.16364638412260746 1103 135-139 0.20437547287622237 1103 140-144 0.2256779983400108 1103 145-149 0.19845915044236762 1103 150-151 0.47764146149531683 2204 1 0.0282597258219468 2204 2 -0.07603964999273671 2204 3 -0.0013512936925437202 2204 4 0.011810183926939999 2204 5 -0.02212731446888938 2204 6 -0.017902427156606393 2204 7 0.020010195247024853 2204 8 -0.048507395585971835 2204 9 -0.02345628288649948 2204 10-14 0.05692877979750932 2204 15-19 0.01760672993238188 2204 20-24 0.09124682867357592 2204 25-29 0.07433075443115911 2204 30-34 0.058183928173846766 2204 35-39 0.020987667485009354 2204 40-44 0.07838441425982978 2204 45-49 0.03650360994537749 2204 50-54 0.1283087213699048 2204 55-59 0.11145863137253542 2204 60-64 0.06786646369991445 2204 65-69 0.01142334103347764 2204 70-74 0.07022656146057216 2204 75-79 0.12297305325849806 2204 80-84 0.09082065933920092 2204 85-89 0.061005564019552594 2204 90-94 0.03107749611606181 2204 95-99 0.09416217489892631 2204 100-104 0.21113643418057393 2204 105-109 0.12947848369125126 2204 110-114 0.1203951044185061 2204 115-119 0.13695604553169716 2204 120-124 0.09991187024386505 2204 125-129 0.20174015901665854 2204 130-134 0.11597847707293596 2204 135-139 0.15324465273195642 2204 140-144 0.14003552699638533 2204 145-149 0.17439624474400972 2204 150-151 0.39639221059625385 2205 1 0.028509017973803452 2205 2 -0.04290686003432853 2205 3 0.02228872463075504 2205 4 0.033262507121243345 2205 5 0.04691108286795753 2205 6 0.09017512972166486 2205 7 0.11720420700063983 2205 8 0.19021041816434092 2205 9 0.10241471133013391 2205 10-14 0.13682744543228864 2205 15-19 0.1327290773564016 2205 20-24 0.1612321531055798 2205 25-29 0.1485491188831034 2205 30-34 0.15329951901759387 2205 35-39 0.15699291960206097 2205 40-44 0.13181354931408862 2205 45-49 0.17297411139242058 2205 50-54 0.12186121842667319 2205 55-59 0.10990348236175862 2205 60-64 0.12468062470973251 2205 65-69 0.13758831401267457 2205 70-74 0.07903564918195372 2205 75-79 0.05874426330753835 2205 80-84 0.10003265120233351 2205 85-89 0.1316861277419079 2205 90-94 0.08411773926563626 2205 95-99 0.12985717754524728 2205 100-104 0.03935954463204183 2205 105-109 0.08260781649601512 2205 110-114 0.06578089373430629 2205 115-119 0.010582347275835957 2205 120-124 0.11608343640497054 2205 125-129 0.2186499364127883 2205 130-134 0.05387428973202191 2205 135-139 0.10640770787260578 2205 140-144 0.12280958675744102 2205 145-149 0.03487306507349075 2205 150-151 0.43912168048014166 1216 1 0.002703346659238548 1216 2 -0.06667384038940583 1216 3 -0.04297496358110209 1216 4 -0.030344488476472975 1216 5 -0.03409186499607131 1216 6 -0.020065721159220118 1216 7 0.044957531951460794 1216 8 0.09887563729943594 1216 9 0.07753250664217148 1216 10-14 0.06874585923340959 1216 15-19 0.08606691507088016 1216 20-24 0.05623091507035838 1216 25-29 0.052647282782970706 1216 30-34 0.05239746777810694 1216 35-39 0.022825663001725616 1216 40-44 -0.019205649059209406 1216 45-49 0.009975486536163203 1216 50-54 -0.011815566250334086 1216 55-59 0.02046418580824394 1216 60-64 -0.00405011148833978 1216 65-69 -0.01984302619793965 1216 70-74 -0.007318044266959589 1216 75-79 -0.017599330296981464 1216 80-84 -0.04421551875098828 1216 85-89 0.026347523397348027 1216 90-94 0.030641461806339976 1216 95-99 0.051030863760686884 1216 100-104 0.14409037954170856 1216 105-109 0.06823007601859388 1216 110-114 0.058812478102787225 1216 115-119 0.01713150400061636 1216 120-124 0.057723615681020135 1216 125-129 0.11801776080110216 1216 130-134 0.0687851771111525 1216 135-139 0.018753555773592723 1216 140-144 0.09625567703240279 1216 145-149 0.04071923247354192 1216 150-151 0.38549927407600393 2206 1 -0.10833612379168045 2206 2 -0.1467718446154862 2206 3 -0.14770864237332404 2206 4 -0.14185991606848347 2206 5 -0.19478848454166808 2206 6 -0.14192200661066323 2206 7 -0.10665435912251553 2206 8 -0.0442897710502308 2206 9 -0.1157284538212835 2206 10-14 -0.1362698570974743 2206 15-19 -0.10999445242324413 2206 20-24 -0.11193761841551009 2206 25-29 -0.12833238380399337 2206 30-34 -0.15411465042203076 2206 35-39 -0.14621004838750196 2206 40-44 -0.15667928805611808 2206 45-49 -0.15951710573563105 2206 50-54 -0.12561156875418078 2206 55-59 -0.11902669220760487 2206 60-64 -0.139711472954545 2206 65-69 -0.13274345488736827 2206 70-74 -0.14620930504000995 2206 75-79 -0.13480763648959737 2206 80-84 -0.13235611935245117 2206 85-89 -0.10778796022146508 2206 90-94 -0.09780312447175277 2206 95-99 -0.1078224966246566 2206 100-104 -0.10856500167074046 2206 105-109 -0.08334630358656625 2206 110-114 -0.14120424398846865 2206 115-119 -0.10655706902435469 2206 120-124 -0.12194088543029835 2206 125-129 -0.01950600770939559 2206 130-134 -0.10223880892958448 2206 135-139 -0.08979522578880506 2206 140-144 -0.06677143092518634 2206 145-149 -0.07406062132771041 2206 150-151 0.2324315852126304 2207 1 -0.1151573015559606 2207 2 -0.19656524272046028 2207 3 -0.15736548740527212 2207 4 -0.1693789052367265 2207 5 -0.1811504171945444 2207 6 -0.18452634355175945 2207 7 -0.17066450925146626 2207 8 -0.14119829788408822 2207 9 -0.17234286744444915 2207 10-14 -0.15325286402536165 2207 15-19 -0.13786007633677144 2207 20-24 -0.1586446357878728 2207 25-29 -0.16588583547096647 2207 30-34 -0.14023275966032855 2207 35-39 -0.17010657168734866 2207 40-44 -0.1730152080560856 2207 45-49 -0.16474786294971722 2207 50-54 -0.1970679652116516 2207 55-59 -0.18864479866677897 2207 60-64 -0.15356972358957677 2207 65-69 -0.16401069634689236 2207 70-74 -0.14749795869091287 2207 75-79 -0.1571938207659329 2207 80-84 -0.17528857091007666 2207 85-89 -0.16399299871322626 2207 90-94 -0.1892132225961305 2207 95-99 -0.1923913581350618 2207 100-104 -0.08140423336275404 2207 105-109 -0.10959940554414516 2207 110-114 -0.13083310830506178 2207 115-119 -0.15450222708464878 2207 120-124 -0.1108547368386823 2207 125-129 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1213 4 -0.4102674724783242 1213 5 -0.30081216711934644 1213 6 -0.5882584500795076 1213 7 -0.6934541613288161 1213 8 -0.6296297837614588 1213 9 -0.6419852931941676 1213 10-14 -0.6842493633354394 1213 15-19 -0.7031632244202939 1213 20-24 -0.7121469466672607 1213 25-29 -0.7698230592686173 1213 30-34 -0.8358338842800919 1213 35-39 -0.8091693241301883 1213 40-44 -0.7018759752626309 1213 45-49 -0.7356955653539572 1213 50-54 -0.7611563223149744 1213 55-59 -0.7128439759275693 1213 60-64 -0.7292321588572435 1213 65-69 -0.6919338575174336 1213 70-74 -0.6904832244783421 1213 75-79 -0.6827736931168573 1213 80-84 -0.7351228338241853 1213 85-89 -0.8416807300460007 1213 90-94 -0.7756928153075435 1213 95-99 -0.7660493088250249 1213 100-104 -0.8117959580453302 1213 105-109 -0.7494899913639586 1213 110-114 -0.8334061078096866 1213 115-119 -0.775580811460614 1213 120-124 -0.8683486825342044 1213 125-129 -0.8866967748496819 1213 130-134 -0.9063950582823637 1213 135-139 -0.9808850580100383 1213 140-144 -1.1354247666077413 1213 145-149 -1.073140232108038 1213 150-151 -2.3803340320243294 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 3.0 10 6.0 11 17.0 12 19.0 13 30.0 14 49.0 15 45.0 16 157.0 17 1236.0 18 3424.0 19 8555.0 20 16347.0 21 25339.0 22 35379.0 23 46663.0 24 58793.0 25 71427.0 26 92878.0 27 139225.0 28 117883.0 29 121803.0 30 137470.0 31 160488.0 32 191219.0 33 231702.0 34 290284.0 35 366602.0 36 488875.0 37 730733.0 38 1430267.0 39 9508418.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.644449012325076 13.549084630127469 7.551872407833839 44.254593949713616 2 19.476507099779326 18.48468697861193 47.49649616000444 14.542309761604304 3 20.949096280876127 23.100513722049193 26.35402594596732 29.596364051107365 4 24.90897955529996 36.23817631472749 19.586581877112533 19.266262252860013 5 25.395659038694507 33.402117084216705 22.63714461348993 18.565079263598864 6 18.060954922532122 36.34930204094671 28.130966584604383 17.458776451916787 7 14.852468782258708 17.387204508849464 45.647841685898136 22.112485022993695 8 17.973104433784577 19.146409832309185 33.010966406566496 29.869519327339745 9 25.10665948598338 19.53610759144303 32.15030455325184 23.206928369321744 10-14 22.769562832006194 28.070308117441158 26.65170473045258 22.508424320100065 15-19 23.025308070515692 26.51702423023946 27.984677291930005 22.47299040731484 20-24 23.163824219970145 27.372534372086538 26.697856629333465 22.765784778609852 25-29 23.5324680499354 27.23799240585191 26.57997926433473 22.64956027987796 30-34 22.360281793283736 27.26351491216954 27.3424036938802 23.033799600666523 35-39 21.683664137495313 28.154982188294454 26.953802208773375 23.207551465436858 40-44 22.731356697397175 27.693486876039415 27.89408196162374 21.68107446493967 45-49 22.389889242665518 27.11264284591457 27.440494587385704 23.056973324034207 50-54 23.130742378291377 27.607925143153466 26.694200936356456 22.5671315421987 55-59 22.515442471456726 27.07645400771512 28.196894742228746 22.211208778599403 60-64 21.508204267693948 28.17699567496944 28.284852787433785 22.02994726990283 65-69 22.327874289546664 28.452693715567367 27.0533397734188 22.166092221467167 70-74 22.057109364011335 28.30013572728468 27.320225447429063 22.32252946127492 75-79 22.5256004974391 27.3994463903769 27.562807479851365 22.512145632332633 80-84 23.49855830773591 27.014025794715735 27.16912491417631 22.318290983372048 85-89 22.650821391200342 28.649743573885907 26.889322638068496 21.81011239684526 90-94 23.644668152005092 26.182650503160577 27.943902006165196 22.22877933866914 95-99 23.24281199175057 28.02602713443359 26.40226298590295 22.328897887912888 100-104 23.780837940504505 26.593855328256627 27.003627513757277 22.621679217481592 105-109 24.257962221269082 28.46109529613388 26.07393512595647 21.20700735664056 110-114 25.201099879665286 28.273869498608516 25.9746163551474 20.550414266578798 115-119 25.17905571661337 29.348851771002494 24.914767766667058 20.557324745717075 120-124 26.080558297940836 28.840882399471457 24.664763103098654 20.413796199489056 125-129 26.763776761317825 28.684836472892865 24.402858889592586 20.14852787619672 130-134 26.750098790580235 28.14518305425488 24.705197181066154 20.39952097409873 135-139 28.09744645422657 28.17552339368004 24.67117378407104 19.05585636802235 140-144 27.98898135927299 28.076638489916117 24.51278803560097 19.421592115209926 145-149 27.692111370053773 28.850378826938922 24.658519985760645 18.798989817246657 150-151 27.329361434675125 29.390961917702985 24.485113891443287 18.7945627561786 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 152.0 1 205.0 2 208.0 3 149.5 4 130.5 5 138.0 6 164.5 7 177.5 8 186.5 9 228.5 10 266.5 11 337.0 12 451.5 13 571.5 14 818.0 15 1530.5 16 4488.0 17 7897.5 18 10464.5 19 10153.5 20 8641.0 21 9366.5 22 11287.0 23 16290.0 24 24097.5 25 39675.0 26 68377.0 27 90680.5 28 92452.0 29 82701.5 30 78207.5 31 79385.0 32 84878.0 33 104486.5 34 109651.0 35 122716.0 36 212017.5 37 301592.0 38 322280.5 39 336113.5 40 398050.0 41 515191.5 42 604694.0 43 585750.0 44 615553.5 45 749202.5 46 959060.5 47 1057472.0 48 1104253.0 49 1155426.5 50 967003.0 51 778130.5 52 663864.5 53 598074.0 54 498839.0 55 297163.5 56 170055.5 57 102490.5 58 52655.5 59 33124.5 60 22142.0 61 21779.0 62 28323.5 63 26485.0 64 16736.5 65 7812.5 66 4420.0 67 2716.5 68 761.5 69 569.0 70 435.5 71 382.5 72 324.0 73 340.0 74 338.0 75 264.0 76 216.5 77 218.0 78 206.5 79 166.0 80 182.5 81 161.0 82 146.0 83 132.0 84 106.0 85 117.0 86 91.0 87 60.5 88 38.5 89 24.5 90 12.5 91 5.5 92 5.0 93 6.0 94 4.0 95 2.0 96 0.5 97 1.0 98 2.5 99 2.5 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.046394704825161386 2 0.05696538421232257 3 0.0716550559650575 4 0.06714377861228625 5 0.0724256157613383 6 0.0 7 8.40610686851784E-4 8 0.0013659923661341492 9 0.0 10-14 0.0 15-19 2.8020356228392803E-6 20-24 1.1908651397066941E-4 25-29 1.989445292215889E-4 30-34 3.432493637978118E-4 35-39 0.0012118804068779888 40-44 7.845699743949985E-5 45-49 0.0011488346053641048 50-54 9.204687021027035E-4 55-59 0.0018003078876742378 60-64 0.0023719231547334506 65-69 0.004043337403757082 70-74 0.004778871754752392 75-79 0.0066226111945806385 80-84 0.00506047633484774 85-89 0.006527341983404104 90-94 0.0030107872767408065 95-99 0.0042226676836187955 100-104 0.0026605328238858963 105-109 0.007728014247790735 110-114 0.010392750125110891 115-119 0.010583288547463961 120-124 0.008867041728474903 125-129 0.006100031550921113 130-134 0.008418716028820617 135-139 0.004893755215288803 140-144 0.002796431551593602 145-149 0.0037323114496219216 150-151 0.0026829491088686108 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 1.4275336E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 8.636773029448047 #Duplication Level Percentage of deduplicated Percentage of total 1 82.31236071930196 7.109131770506661 2 8.776102585094886 1.515944122212336 3 2.6866554285880677 0.6961209943514882 4 1.2655146112190434 0.43719849850196263 5 0.9463573183419471 0.4086736681638255 6 0.6777947727027318 0.3512375767427894 7 0.2787513071484797 0.16852582400523644 8 0.230139457505948 0.15901298076793413 9 0.19231203408473316 0.14948598502991853 >10 1.572246703362744 2.733205531122763 >50 0.2772043333622229 1.7149222395167592 >100 0.4666531567603753 9.409583087862815 >500 0.11918281232620034 7.25880865402893 >1k 0.1580759433283274 29.868772267816123 >5k 0.027127788795117055 16.037388248841648 >10k+ 0.01352102807742521 21.981988550528822 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 105466 0.7387987224959188 Illumina Single End PCR Primer 1 (100% over 50bp) GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG 76790 0.5379207886945708 No Hit CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG 57403 0.402113127144608 No Hit CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC 56213 0.3937770711666611 No Hit CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG 50227 0.3518446080708713 No Hit GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA 46901 0.3285456818669627 No Hit CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG 40480 0.28356600503133517 No Hit CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA 38255 0.26797968187929166 No Hit CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT 36662 0.2568205750113342 No Hit GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC 35911 0.2515597531294535 No Hit GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG 34340 0.24055475822075223 No Hit GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT 34129 0.2390766844297045 No Hit ATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAG 31714 0.22215939435681234 No Hit ATCAAACCGTACTCACTTCACATTACAGCCCGACCGGCCCAGTCCTTAGA 30771 0.21555359537596872 No Hit CTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTGGATC 30509 0.213718262043009 No Hit GTCAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTGACTTTCGTTC 30070 0.21064302794694287 No Hit GTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAATCACCG 29614 0.20744870733690612 No Hit GAACAATCCAACGCTTGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCG 29269 0.20503195161220722 No Hit CTCAGATGGTAGCTTCGCACCATTGGCCGATCGGCCAAGCACATGAACCA 29070 0.2036379388898447 No Hit GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA 28941 0.202734282401479 No Hit GTCGATCCTAAGATATTGGCTAGTGCCGTTTTTTAAAATAAAATAGAGGA 28594 0.20030351649866596 No Hit CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC 28479 0.19949793125709964 No Hit CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG 27444 0.192247664083003 No Hit CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC 25876 0.18126368444147306 No Hit CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA 25756 0.18042307375462127 No Hit CAGAAATCAGAGACAGTCAACGCCCGCAAGGGCCATCTGTCTCCTCTGTT 25635 0.17957545797871238 No Hit CTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAAC 24307 0.17027269971088596 No Hit CTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGA 24039 0.16839533584358365 No Hit GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC 23497 0.16459857757463642 No Hit CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT 22941 0.16070374805888982 No Hit CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC 22902 0.160430549585663 No Hit CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT 22029 0.15431510683881627 No Hit GGGAGTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCT 21592 0.15125388292086434 No Hit GTCAATTTCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGAACCGA 21324 0.14937651905356203 No Hit CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA 21277 0.1490472798678784 No Hit CCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTT 21239 0.1487810864837087 No Hit CGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGT 21100 0.14780737910477204 No Hit GTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCTAAGG 20760 0.14542564882535866 No Hit CACGCTTTCGCGGTCTGTATTCGTACTGGAAATCAAGATCAAGCGAGCTT 20655 0.14469011447436333 No Hit CAACGGTTGAGTATAAGTCTCCCGCTCAAGCGCCATCCATTTTCAGGGCT 20505 0.1436393511157986 No Hit GTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAATTAAAA 20420 0.14304391854594525 No Hit CTCACTTCACATTACAGCCCGACCGGCCCAGTCCTTAGAGCCAATCCTTA 20333 0.14243447579797772 No Hit GTCGCAATGGTTAGCGTTGAAGACTTTGGGCGTAGGCCCGGTTGAAGCTT 20200 0.14150279895338366 No Hit CTGGAATCGGTTCAGCCGGAGATAGGGATGTTGTTTCCGTAAAGCGCCAC 20156 0.14119457503487132 No Hit CTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCT 20095 0.14076726460238834 No Hit CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG 19918 0.13952736383928196 No Hit CTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATTC 19914 0.13949934348305357 No Hit GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT 19893 0.1393522366128545 No Hit CTAGAGTCTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAAC 19764 0.13844858012448882 No Hit AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG 19751 0.13835751396674656 No Hit CACAGACCTGTTATTGCTCAACTTCATGCGGCTAAACACCGCTTGTCCCT 19546 0.13692147071004143 No Hit TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT 19303 0.13521923406916658 No Hit CGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGGTA 19299 0.13519121371293816 No Hit CTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAACCGAGATC 19021 0.13324379895506488 No Hit CTGACTTTCGTTCTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCA 18981 0.13296359539278094 No Hit CTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAA 18637 0.13055384475713916 No Hit CTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTTCATGTGCTTGGC 18544 0.12990237147482903 No Hit GCCAGATCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTA 18491 0.12953110175480284 No Hit GTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTAC 18374 0.12871150633512235 No Hit CTCACGTTCCCTATTGGTGGGTGAACAATCCAACGCTTGGCGAATTCTGC 18283 0.1280740432309264 No Hit GTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAA 18230 0.1277027735109002 No Hit CCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTC 18037 0.12635079132288027 No Hit GTTAATATTCCCGTACCTGGACACGGAGATAAATGCTCATCGTGAGAATT 17894 0.1253490635877152 No Hit CTTGACTCTAGTCCGACTCTGTGAAGAGTCATGAGAGGTGTAGCATAAGT 17812 0.12477464628503315 No Hit CTCGGATTTTCATGGATCGAACAAAGCGCACGAGACACCACAGGAACCGT 17594 0.12324753687058575 No Hit CTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTT 17264 0.12093585748174335 No Hit GTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGGCAACCA 17168 0.1202633689322619 No Hit CGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCCACCAAAG 17096 0.11975900252015083 No Hit GTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTGGAT 16974 0.11890438165518485 No Hit GTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTG 16911 0.11846306104458766 No Hit CTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTGC 16691 0.11692194145202607 No Hit CTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAG 16153 0.11315320353930723 No Hit ATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGGATCGACTGACCC 16110 0.11285198470985201 No Hit CTTTCATCGATTCTTTACTTATTCGGTAAAACGAGAATTGAAATATTATA 15962 0.11181523152940147 No Hit GTCAGATAGGTGATTAACGTTCACATCGAGATGTAACTTTATTGCATACA 15902 0.11139492618597559 No Hit CTTGCTAGAAACCTTCTAAAAAATTCAAAAGGATCGATAGGCCACGCTTT 15793 0.11063137147875189 No Hit GTGAAATACCACTACTTTCATCGATTCTTTACTTATTCGGTAAAACGAGA 15735 0.1102250763134402 No Hit GGCGAATTCTGCTTCGCAATGATAGGAAGAGCCGACATCGAAGGATCAAA 15571 0.10907624170807609 No Hit CGAGACTCTAGCCTACTAAATAGTTACTGGATAATTTTATCGTCCAGACA 15533 0.10881004832390637 No Hit ATTTTTTCAAGGTAAACTTGCTAGCCACCATATGCAACCAATTAAGGTAA 15512 0.10866294145370728 No Hit CGGAGATAGGGATGTTGTTTCCGTAAAGCGCCACGGTTCCTGTGGTGTCT 15414 0.10797644272611166 No Hit GTTGAGCTTGACTCTAGTCCGACTCTGTGAAGAGTCATGAGAGGTGTAGC 15225 0.1066524808943201 No Hit GTTTACGGTCAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTGACT 15129 0.10597999234483867 No Hit GCCAGTTCTAAGTTGACTGTTTAACGCCGGCCGAAATATCAAATAAAACA 15064 0.10552466155612729 No Hit GAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGC 15009 0.10513938165798689 No Hit GTTTTATTTGATATTTCGGCCGGCGTTAAACAGTCAACTTAGAACTGGCA 14762 0.10340912466088364 No Hit CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT 14740 0.10325501270162749 No Hit GTTGAAGCTTCCATTGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAG 14688 0.10289074807065839 No Hit CTTTAACTCAACGTTTGGTTCATCCCACAGCGCCAGTTCTGCTTACCAAA 14660 0.10269460557705962 No Hit GTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATTTTCATGGATC 14659 0.10268760048800253 No Hit GGCATTCGTATCGCTGCGTGAGAGGTGAAATTCTTGGACCGTAGCGAGAC 14601 0.10228130532269084 No Hit GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC 14507 0.1016228269513236 No Hit CTCCGTGTCCAGGTACGGGAATATTAACCCGTTTCCCTTTCGATTTATCA 14420 0.10101338420335605 No Hit AAACAGTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAAT 14323 0.10033389056481752 No Hit CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG 14318 0.10029886511953204 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0016602061065322735 0.0 0.0 2 0.0017372620861603539 0.0 0.0 3 0.0018983791344736123 0.0 0.0 4 0.0022486335873285225 0.0 0.0 5 0.0023186844778995044 0.0 0.0 6 0.002381730279413388 0.0 0.0 7 0.0024868066152698614 0.0 0.0 8 0.0025568575058408432 0.0 0.0 9 0.004175033078030527 0.0 0.0 10-11 0.0050121412203537625 0.0 0.0 12-13 0.00537290330679432 0.0 0.0 14-15 0.006836966919727844 0.0 0.0 16-17 0.008108390583591168 0.0 0.0 18-19 0.008910473280628911 0.0 0.0 20-21 0.009635499998038574 0.0 0.0 22-23 0.010125856232035449 0.0 3.5025445285491E-6 24-25 0.01097347200794433 0.0 7.0050890570982E-6 26-27 0.012805302796375512 0.0 7.0050890570982E-6 28-29 0.014770230276891557 0.0 7.0050890570982E-6 30-31 0.02421309032585993 0.0 7.0050890570982E-6 32-33 0.027691117042709188 0.0 7.0050890570982E-6 34-35 0.031022036889359384 0.0 7.0050890570982E-6 36-37 0.03506397327530504 0.0 7.0050890570982E-6 38-39 0.039498194648448205 0.0 7.0050890570982E-6 40-41 0.044615412204658436 0.0 7.0050890570982E-6 42-43 0.05089197199981843 0.0 7.0050890570982E-6 44-45 0.058439955458841744 3.5025445285491E-6 7.0050890570982E-6 46-47 0.06739946436287034 7.0050890570982E-6 7.0050890570982E-6 48-49 0.0784464898059142 7.0050890570982E-6 7.0050890570982E-6 50-51 0.09217296181329812 7.0050890570982E-6 7.0050890570982E-6 52-53 0.11004994908701274 7.0050890570982E-6 7.0050890570982E-6 54-55 0.1314820190572047 7.0050890570982E-6 7.0050890570982E-6 56-57 0.1563711004770746 7.0050890570982E-6 7.0050890570982E-6 58-59 0.18409023787601217 7.0050890570982E-6 1.40101781141964E-5 60-61 0.21826456484106574 1.05076335856473E-5 1.40101781141964E-5 62-63 0.26007443887835635 1.40101781141964E-5 1.40101781141964E-5 64-65 0.3098596068071533 1.40101781141964E-5 1.40101781141964E-5 66-67 0.36947991977211603 1.40101781141964E-5 1.40101781141964E-5 68-69 0.44236086632216576 1.40101781141964E-5 1.40101781141964E-5 70-71 0.533871847219568 1.40101781141964E-5 1.40101781141964E-5 72-73 0.6518830800199729 1.40101781141964E-5 1.40101781141964E-5 74-75 0.7993822352062326 1.40101781141964E-5 1.40101781141964E-5 76-77 0.963227765707231 1.40101781141964E-5 1.40101781141964E-5 78-79 1.1526628865338089 1.40101781141964E-5 1.40101781141964E-5 80-81 1.4007376078573563 1.40101781141964E-5 1.40101781141964E-5 82-83 1.6867694042367898 1.40101781141964E-5 1.40101781141964E-5 84-85 1.9964188583722304 1.40101781141964E-5 1.40101781141964E-5 86-87 2.371530869746253 1.40101781141964E-5 1.40101781141964E-5 88-89 2.8052509587164884 1.40101781141964E-5 1.40101781141964E-5 90-91 3.292917238515437 1.40101781141964E-5 1.40101781141964E-5 92-93 3.8526798948900396 1.40101781141964E-5 1.40101781141964E-5 94-95 4.49945276244286 1.40101781141964E-5 1.40101781141964E-5 96-97 5.196623743216972 1.40101781141964E-5 1.40101781141964E-5 98-99 5.987662917356201 1.40101781141964E-5 1.40101781141964E-5 100-101 6.81293245917294 1.40101781141964E-5 1.40101781141964E-5 102-103 7.692022100215365 1.40101781141964E-5 1.40101781141964E-5 104-105 8.684884895178648 1.40101781141964E-5 1.7512722642745503E-5 106-107 9.75792793948948 1.40101781141964E-5 2.1015267171294604E-5 108-109 10.852718282778072 1.40101781141964E-5 2.1015267171294604E-5 110-111 12.009072851244973 1.40101781141964E-5 2.1015267171294604E-5 112-113 13.385548333153068 1.40101781141964E-5 2.1015267171294604E-5 114-115 14.744304442291234 1.40101781141964E-5 2.45178116998437E-5 116-117 16.177675257521084 1.40101781141964E-5 2.80203562283928E-5 118-119 17.677216143984282 1.40101781141964E-5 2.80203562283928E-5 120-121 19.098888460488777 1.40101781141964E-5 2.80203562283928E-5 122-123 20.65693585075686 1.40101781141964E-5 2.80203562283928E-5 124-125 22.295969075614053 1.40101781141964E-5 2.80203562283928E-5 126-127 23.781426230527956 1.40101781141964E-5 2.80203562283928E-5 128-129 25.34797779891135 1.40101781141964E-5 2.80203562283928E-5 130-131 26.868530450001316 1.40101781141964E-5 2.80203562283928E-5 132-133 28.34321728048993 1.40101781141964E-5 3.5025445285491006E-5 134-135 29.761765327274958 2.1015267171294604E-5 3.5025445285491006E-5 136-137 31.186054044542278 2.1015267171294604E-5 3.5025445285491006E-5 138-139 32.49197076692275 2.1015267171294604E-5 3.5025445285491006E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAATC 4155 0.0 103.7189 2 CTTGTTA 9075 0.0 94.8141 1 GTCCAAT 4660 0.0 93.72285 1 CCAGACG 3560 0.0 92.448494 6 CAGACGT 3815 0.0 86.45914 7 GTTACGA 10255 0.0 83.62008 4 TTACGAC 10550 0.0 81.419426 5 TCAAAGG 6410 0.0 74.30215 7 ATCAAAG 6240 0.0 72.84117 6 CCTGGCC 8215 0.0 70.56153 5 CAAAGGA 6185 0.0 70.20711 8 ACCTGGC 8660 0.0 66.851906 4 CCGGCAT 1015 0.0 62.900124 1 CTAGCAT 3120 0.0 62.783287 1 CAACCAG 5340 0.0 59.779724 3 TGGCTCA 2855 0.0 59.71786 3 CTCGCGT 480 0.0 57.43508 1 GTTAGTT 20915 0.0 54.806755 1 ATACGCA 3495 0.0 54.758396 8 GATAGGT 4400 0.0 54.246532 5 >>END_MODULE