##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H55MKBCXX l02n02 3_ym.3420000000ed11.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 29531137 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.975841702268355 35.0 35.0 35.0 32.0 35.0 2 33.99347928933451 35.0 35.0 35.0 32.0 35.0 3 33.83229416463037 35.0 35.0 35.0 31.0 35.0 4 33.961982025954505 35.0 35.0 35.0 32.0 35.0 5 33.92514517812165 35.0 35.0 35.0 32.0 35.0 6 38.411360998392986 40.0 39.0 40.0 36.0 40.0 7 38.544471416728726 40.0 39.0 40.0 36.0 40.0 8 38.52612230947965 40.0 39.0 40.0 36.0 40.0 9 38.58822340636596 40.0 39.0 40.0 36.0 40.0 10-14 38.582641772309685 40.0 39.0 40.0 36.0 40.0 15-19 38.5579820512837 40.0 39.0 40.0 36.0 40.0 20-24 38.573144738721034 40.0 39.0 40.0 36.0 40.0 25-29 38.56002832535707 40.0 39.0 40.0 36.0 40.0 30-34 38.537289011256156 40.0 39.0 40.0 36.0 40.0 35-39 38.50525935388129 40.0 39.0 40.0 36.0 40.0 40-44 38.425729175276935 40.0 39.0 40.0 36.0 40.0 45-49 38.33186185821426 40.0 39.0 40.0 36.0 40.0 50-54 38.30874622267338 40.0 39.0 40.0 35.6 40.0 55-59 38.289320895433185 40.0 39.0 40.0 35.0 40.0 60-64 38.29412340608491 40.0 39.0 40.0 35.0 40.0 65-69 38.24108361963849 40.0 39.0 40.0 35.0 40.0 70-74 38.161139328973356 40.0 39.0 40.0 35.0 40.0 75-79 38.12653589328444 40.0 39.0 40.0 34.8 40.0 80-84 38.08708469301403 40.0 39.0 40.0 34.2 40.0 85-89 38.03915844486448 40.0 39.0 40.0 34.0 40.0 90-94 38.037500858839266 40.0 39.0 40.0 34.0 40.0 95-99 37.99783253181211 40.0 39.0 40.0 34.0 40.0 100-104 36.90134706970476 38.8 37.6 39.4 32.0 39.6 105-109 37.95571035412555 40.0 39.0 40.0 34.0 40.0 110-114 38.00519928508002 40.0 39.0 40.0 34.0 40.0 115-119 37.92701286780797 40.0 39.0 40.0 34.0 40.0 120-124 37.84284840776703 40.0 39.0 40.0 34.0 40.0 125-129 37.738620317937645 40.0 39.0 40.0 34.0 40.0 130-134 37.69845256550739 40.0 39.0 40.0 34.0 40.0 135-139 37.61243809203824 40.0 39.0 40.0 34.0 40.0 140-144 37.501264566955214 40.0 39.0 40.0 34.0 40.0 145-149 37.34283356580547 40.0 39.0 40.0 31.4 40.0 150-151 35.467367866669 38.5 35.0 39.5 23.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.3604651222107975 2201 2 0.3414993214196045 2201 3 0.3159024125536902 2201 4 0.3457919901469211 2201 5 0.30039997276407604 2201 6 0.5536249087801153 2201 7 0.5359285595491983 2201 8 0.6040485252697252 2201 9 0.5452702245918388 2201 10-14 0.5354709842685779 2201 15-19 0.5314948801639048 2201 20-24 0.5311895734633296 2201 25-29 0.5272797015822732 2201 30-34 0.5301711023993505 2201 35-39 0.516423268149623 2201 40-44 0.5489380874196783 2201 45-49 0.5094283307369238 2201 50-54 0.5040779753445577 2201 55-59 0.5319257823711112 2201 60-64 0.5022375202006444 2201 65-69 0.4839310610987084 2201 70-74 0.46656577554259115 2201 75-79 0.49097135704773365 2201 80-84 0.5256726505017255 2201 85-89 0.5446873461218544 2201 90-94 0.5356029897387629 2201 95-99 0.49758511775055325 2201 100-104 0.452636396498896 2201 105-109 0.5159102963044191 2201 110-114 0.5340776755956824 2201 115-119 0.5181045818773171 2201 120-124 0.5555604960594707 2201 125-129 0.5951936705533853 2201 130-134 0.5421488716559537 2201 135-139 0.5188787264572667 2201 140-144 0.5595504437113306 2201 145-149 0.5477788416512368 2201 150-151 0.6184491080761916 1101 1 0.32056554754707633 1101 2 0.3124772391827477 1101 3 0.33618864151760164 1101 4 0.3269202794957593 1101 5 0.3375869895088712 1101 6 0.5160941684397145 1101 7 0.4471171341095257 1101 8 0.5514159699031467 1101 9 0.49099956826693614 1101 10-14 0.5097588219713884 1101 15-19 0.5120784255174513 1101 20-24 0.4931836181085387 1101 25-29 0.5144848725350073 1101 30-34 0.5063688857683388 1101 35-39 0.5180250565051381 1101 40-44 0.5104902399333042 1101 45-49 0.5312739903312789 1101 50-54 0.4982680677562925 1101 55-59 0.5111595475136923 1101 60-64 0.4821169115197179 1101 65-69 0.5238448581941668 1101 70-74 0.5046587117309898 1101 75-79 0.49824401439418864 1101 80-84 0.52990827097166 1101 85-89 0.5389059731540868 1101 90-94 0.5292161651043443 1101 95-99 0.5337361207546465 1101 100-104 0.5778056876608559 1101 105-109 0.5706164461058876 1101 110-114 0.5351673603713181 1101 115-119 0.5439393530500922 1101 120-124 0.5576592098994482 1101 125-129 0.5867380384379643 1101 130-134 0.5725025872229352 1101 135-139 0.589356559445811 1101 140-144 0.5964021975718907 1101 145-149 0.6180806469909896 1101 150-151 0.7333875246327537 2202 1 0.2513383685804058 2202 2 0.24862176168667105 2202 3 0.263713917047923 2202 4 0.20674313662406263 2202 5 0.24525042509627326 2202 6 0.38161861310815937 2202 7 0.36735972119032567 2202 8 0.4511133619535812 2202 9 0.424410697455194 2202 10-14 0.41677713707969133 2202 15-19 0.4230873328093381 2202 20-24 0.39548870511683987 2202 25-29 0.41477081665010473 2202 30-34 0.40506544257244315 2202 35-39 0.4219698880258207 2202 40-44 0.4072320115272774 2202 45-49 0.43601388415461884 2202 50-54 0.3996670021163169 2202 55-59 0.41058157193590716 2202 60-64 0.43349543339522967 2202 65-69 0.4251649942247724 2202 70-74 0.40989877642964956 2202 75-79 0.4148348250272633 2202 80-84 0.43464834027102484 2202 85-89 0.44264237353933567 2202 90-94 0.4098405599454722 2202 95-99 0.41170541630020807 2202 100-104 0.4905798316262775 2202 105-109 0.4361785198182915 2202 110-114 0.4077075376518735 2202 115-119 0.43740439113389584 2202 120-124 0.37624751916818866 2202 125-129 0.4994847240886884 2202 130-134 0.46734917205505155 2202 135-139 0.46066220670931557 2202 140-144 0.4905898590381099 2202 145-149 0.49761413428906565 2202 150-151 0.6141509290660281 1102 1 0.21164526768627212 1102 2 0.1551336662888687 1102 3 0.18893826510029044 1102 4 0.17894956364532533 1102 5 0.20624452637385815 1102 6 0.31600200746499496 1102 7 0.27208913947889357 1102 8 0.38412935306440943 1102 9 0.35339604368346045 1102 10-14 0.3621602406708462 1102 15-19 0.36043486509341705 1102 20-24 0.3430924912067468 1102 25-29 0.34997849552974003 1102 30-34 0.3579288301322521 1102 35-39 0.3640369179311804 1102 40-44 0.34306063750743476 1102 45-49 0.3560047366294725 1102 50-54 0.33434140662722456 1102 55-59 0.31230332325447563 1102 60-64 0.2537942105374569 1102 65-69 0.2949991358956652 1102 70-74 0.28375600179787597 1102 75-79 0.3058354141035764 1102 80-84 0.3200551446920059 1102 85-89 0.35472866449102725 1102 90-94 0.31064222203189473 1102 95-99 0.2824663261293452 1102 100-104 0.3829819637513694 1102 105-109 0.32996005736668366 1102 110-114 0.3390960096258411 1102 115-119 0.32460306972725306 1102 120-124 0.342208533915354 1102 125-129 0.37741290581251974 1102 130-134 0.3012296061907662 1102 135-139 0.3291521014150476 1102 140-144 0.2898152603801307 1102 145-149 0.3768330062228671 1102 150-151 0.5632972475307412 2203 1 0.1398467705014923 2203 2 0.12689579143800955 2203 3 0.10511519226683674 2203 4 0.13612560102315285 2203 5 0.12462301740651327 2203 6 0.22570613347807011 2203 7 0.2129802571759214 2203 8 0.3138715870610582 2203 9 0.2761469262969882 2203 10-14 0.2815340896909575 2203 15-19 0.2902670526473514 2203 20-24 0.30711652051459737 2203 25-29 0.2872561876858555 2203 30-34 0.2883521747013589 2203 35-39 0.2863430890881631 2203 40-44 0.25699711624744737 2203 45-49 0.2593077661420935 2203 50-54 0.22198675095371811 2203 55-59 0.2667283766836377 2203 60-64 0.2596401763459042 2203 65-69 0.24210766859786048 2203 70-74 0.2500541213843732 2203 75-79 0.26004996100952127 2203 80-84 0.24315612726428526 2203 85-89 0.2630760567706858 2203 90-94 0.25641909574388677 2203 95-99 0.23638351871910146 2203 100-104 0.5288248877680459 2203 105-109 0.2637616875014075 2203 110-114 0.24580583267726297 2203 115-119 0.2785141116111518 2203 120-124 0.2414999955175432 2203 125-129 0.3405464718115354 2203 130-134 0.3086744344651038 2203 135-139 0.2580262909532536 2203 140-144 0.3311441117463403 2203 145-149 0.3567311153217787 2203 150-151 0.6961703523702951 1103 1 0.08225350995825664 1103 2 0.10083194018365305 1103 3 0.08449739161143555 1103 4 0.10752152381897417 1103 5 0.1206455878437609 1103 6 0.13180669886592966 1103 7 0.19184628627206024 1103 8 0.2680756792615284 1103 9 0.19684884984277318 1103 10-14 0.17221851529900079 1103 15-19 0.1885374945894327 1103 20-24 0.15954808053656677 1103 25-29 0.1417312848052319 1103 30-34 0.17212858384228014 1103 35-39 0.1916155513582538 1103 40-44 0.17854798352946943 1103 45-49 0.1813984548043024 1103 50-54 0.09760239023981399 1103 55-59 0.11389679152608068 1103 60-64 0.16549248834260766 1103 65-69 0.1495070655720241 1103 70-74 0.1677363152915632 1103 75-79 0.15937536570856992 1103 80-84 0.19825278355673248 1103 85-89 0.20089466225812913 1103 90-94 0.1962141653410967 1103 95-99 0.18441040570126432 1103 100-104 0.29348868186127675 1103 105-109 0.21024296259985675 1103 110-114 0.1995128484289026 1103 115-119 0.1593533927576516 1103 120-124 0.16566054001465602 1103 125-129 0.25915854508549785 1103 130-134 0.18818805462358767 1103 135-139 0.22186149988730364 1103 140-144 0.25121277300101497 1103 145-149 0.24003505423856097 1103 150-151 0.4859138262806084 2204 1 0.028487653972206317 2204 2 -0.05558770605703245 2204 3 -0.007707972284798359 2204 4 0.03238917931209784 2204 5 -0.00778363452821651 2204 6 -0.01977229039643902 2204 7 0.01892588525969785 2204 8 -0.07863908138367748 2204 9 -0.07182631699031816 2204 10-14 0.04972490997476342 2204 15-19 -0.0036927425232349265 2204 20-24 0.061447059939524706 2204 25-29 0.06551352447004177 2204 30-34 0.05327176638477482 2204 35-39 -0.019654851561334397 2204 40-44 0.048160033678357195 2204 45-49 0.0010917356068844697 2204 50-54 0.12644762383520458 2204 55-59 0.11580375756237515 2204 60-64 0.059623257109301164 2204 65-69 0.010637868217820312 2204 70-74 0.07797523189824318 2204 75-79 0.11889153787774376 2204 80-84 0.08424003529205493 2204 85-89 0.05756671228058252 2204 90-94 0.02939869597948075 2204 95-99 0.08959801152679603 2204 100-104 0.21324108618912163 2204 105-109 0.12488917730581761 2204 110-114 0.11119817427010759 2204 115-119 0.1001547866759438 2204 120-124 0.09821748873923752 2204 125-129 0.1803140041404916 2204 130-134 0.10045064843368579 2204 135-139 0.12040815045966724 2204 140-144 0.14469533509151944 2204 145-149 0.14466077801021981 2204 150-151 0.3439868930062815 2205 1 -0.001300889845069264 2205 2 -0.03687331030398866 2205 3 0.04796223701899294 2205 4 0.0654536569480797 2205 5 0.06785399668876124 2205 6 0.053450664042216545 2205 7 0.11246767018389647 2205 8 0.1762910996122855 2205 9 0.11892009951163374 2205 10-14 0.14217246810326856 2205 15-19 0.12147548305537015 2205 20-24 0.16535698722607606 2205 25-29 0.15924439073869934 2205 30-34 0.15263526681276574 2205 35-39 0.16262573556724647 2205 40-44 0.0960397285117125 2205 45-49 0.15455119830241415 2205 50-54 0.099571093369768 2205 55-59 0.08480977840897452 2205 60-64 0.1119555031078292 2205 65-69 0.1200416388075638 2205 70-74 0.05424934866950082 2205 75-79 0.05586758594893837 2205 80-84 0.09021773854607318 2205 85-89 0.08619175744148322 2205 90-94 0.030975355604248023 2205 95-99 0.07995017143114325 2205 100-104 0.008333484613707753 2205 105-109 0.05238419876407363 2205 110-114 0.06736204922615485 2205 115-119 -0.012962151971514402 2205 120-124 0.08975900255254743 2205 125-129 0.1872646710853587 2205 130-134 0.032642826117751156 2205 135-139 0.07982330873989696 2205 140-144 0.07174022137579072 2205 145-149 0.009238700785608955 2205 150-151 0.4450337427005593 1216 1 0.04201137387612164 1216 2 -0.04365035274173579 1216 3 0.019064508310009387 1216 4 -0.003615300470265481 1216 5 -0.02251368850932778 1216 6 -0.008351570281256215 1216 7 0.04925516944611985 1216 8 0.13903915497579789 1216 9 0.11359871953699496 1216 10-14 0.11948169144986309 1216 15-19 0.10978008731109412 1216 20-24 0.07723092497201378 1216 25-29 0.05864047946516848 1216 30-34 0.07915487494748419 1216 35-39 0.0389071535283918 1216 40-44 0.013594497671206796 1216 45-49 0.03226653741899099 1216 50-54 0.027524717309226787 1216 55-59 0.04423623387282305 1216 60-64 0.02940525288859419 1216 65-69 0.0022618527989948234 1216 70-74 0.054524675837349434 1216 75-79 0.03392631480308239 1216 80-84 0.022046921212421466 1216 85-89 0.08610798401128505 1216 90-94 0.06294502939861246 1216 95-99 0.10735895896058878 1216 100-104 0.1906127959513455 1216 105-109 0.10362504998869326 1216 110-114 0.09159507238554454 1216 115-119 0.04712612534108018 1216 120-124 0.11277797925561828 1216 125-129 0.13937080807352942 1216 130-134 0.09572716299076234 1216 135-139 0.06139888755066636 1216 140-144 0.12313996723693066 1216 145-149 0.10994829981187593 1216 150-151 0.41420971592269495 2206 1 -0.11241525267076469 2206 2 -0.16575921537809535 2206 3 -0.1918132172598348 2206 4 -0.1322003878602871 2206 5 -0.1808520574885648 2206 6 -0.17839120602852887 2206 7 -0.1708378292132906 2206 8 -0.08230412644346075 2206 9 -0.13216505956907554 2206 10-14 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-0.7341359201420516 1213 25-29 -0.8234296580838958 1213 30-34 -0.8679869887617571 1213 35-39 -0.8173946512235091 1213 40-44 -0.7119954462020246 1213 45-49 -0.7382113942920654 1213 50-54 -0.7719590562935821 1213 55-59 -0.7268861545400753 1213 60-64 -0.7606384974479425 1213 65-69 -0.7064756553381883 1213 70-74 -0.7258162557253058 1213 75-79 -0.7275931570354217 1213 80-84 -0.7545683696172176 1213 85-89 -0.8696490642295345 1213 90-94 -0.8285959381591113 1213 95-99 -0.8089293493052097 1213 100-104 -0.8413866819712439 1213 105-109 -0.7790006140319363 1213 110-114 -0.9141230649109104 1213 115-119 -0.7976458659756247 1213 120-124 -0.920171769523499 1213 125-129 -0.9005288637320348 1213 130-134 -0.9327399592924408 1213 135-139 -0.9881601656412684 1213 140-144 -1.1850011934490254 1213 145-149 -1.1525800697478346 1213 150-151 -2.4681135166846815 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 4.0 10 14.0 11 42.0 12 48.0 13 57.0 14 103.0 15 117.0 16 362.0 17 2808.0 18 8617.0 19 19577.0 20 36261.0 21 57248.0 22 80257.0 23 106344.0 24 134474.0 25 165743.0 26 219767.0 27 295782.0 28 254811.0 29 272732.0 30 312270.0 31 364123.0 32 428220.0 33 513909.0 34 631201.0 35 785821.0 36 1034730.0 37 1533580.0 38 3008056.0 39 1.9264059E7 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.06773202123541 13.06733618569362 7.833241167072513 42.031690625998465 2 20.025863374877343 19.110741588075726 45.06584142214612 15.797553614900806 3 22.354677103020567 22.654074698873323 25.012064585868288 29.979183612237826 4 25.79343853948653 36.04630638503636 18.413297430659107 19.746957644818004 5 25.7337282778537 32.94304266098897 22.062147170262726 19.261081890894598 6 18.58296549841613 35.431270390977495 28.124362431422806 17.86140167918357 7 15.224960762234463 16.8288388205139 45.08780831009296 22.858392107158675 8 18.49287729886446 18.641698589072714 31.768232140353092 31.09719197170973 9 25.886392386449597 20.10482698312632 30.715210863706332 23.293569766717752 10-14 23.48277142190631 27.75364592294567 26.038590386817816 22.724992268330205 15-19 23.51939896276796 26.067276600091194 27.601784470489516 22.811539966651335 20-24 23.83396354801647 26.829444840742273 26.11837957628725 23.21821203495401 25-29 24.21205898142291 26.599491912834083 26.04983836246853 23.138610743274477 30-34 22.891345488489907 26.54333283990881 26.87063197983241 23.694689691768872 35-39 22.30750289382842 27.254847928752728 26.71386249092997 23.72378668648888 40-44 23.35325322821392 26.917343751994714 27.6246337210521 22.104769298739267 45-49 23.13189901935306 26.256166140242122 27.105031307119194 23.506903533285623 50-54 23.698562599538697 26.90214780733145 26.35516053756363 23.04412905556622 55-59 23.104210324547996 26.51262795575333 27.571938054299522 22.811223665399147 60-64 22.20627828858018 27.713011388790314 27.52133787556869 22.559372447060817 65-69 22.86730172145024 27.83302125081762 26.5678176709807 22.731859356751443 70-74 22.76231357434542 27.51439899406925 26.86883058992843 22.8544568416569 75-79 23.151239658248883 26.64153926008217 27.063828587697635 23.14339249397131 80-84 23.902194223115313 26.266527463407467 26.985457509164167 22.84582080431305 85-89 22.92974983330192 27.812719277086018 26.63211168582112 22.625419203790948 90-94 23.877098044083546 25.395524335167124 27.68895945585015 23.03841816489918 95-99 23.266765953842807 27.33307598902791 26.36091119931646 23.039246857812824 100-104 23.677208012348864 25.977453094883895 26.872753950783185 23.472584941984056 105-109 24.125258020692804 27.77913148291955 25.906016496445922 22.189593999941724 110-114 25.0500723391862 27.65419634504351 25.707766512462417 21.587964803307873 115-119 24.895265385848738 28.968114998565696 24.67577988984869 21.46083972573687 120-124 25.744448933225456 28.445096868738617 24.510116561419633 21.30033763661629 125-129 26.467950391604678 28.344798995496117 24.008501023653032 21.17874958924617 130-134 26.567987573318685 27.78412819537165 24.40673313788028 21.241151093429387 135-139 28.01775369048754 28.104371083489514 24.102315521339833 19.77555970468311 140-144 28.09638438313739 27.830344819001212 24.052703020718123 20.02056777714327 145-149 28.164840087752186 28.223533665468143 24.263205487765873 19.3484207590138 150-151 28.006911876704393 28.35513521183136 24.265404416408487 19.37254849505576 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 316.0 1 274.0 2 255.5 3 252.0 4 221.0 5 238.0 6 270.0 7 289.0 8 333.0 9 433.5 10 509.5 11 598.5 12 722.0 13 914.0 14 1320.5 15 2316.5 16 5489.5 17 8910.0 18 11538.5 19 11316.5 20 9911.0 21 11372.0 22 14315.5 23 18578.5 24 25862.5 25 44515.0 26 76115.5 27 96293.5 28 100345.5 29 94436.5 30 92092.0 31 98376.0 32 112700.5 33 143626.5 34 155713.0 35 191583.0 36 363464.5 37 516978.0 38 549286.5 39 589687.0 40 703659.0 41 967218.0 42 1177814.5 43 1143061.0 44 1226956.5 45 1547771.5 46 2006709.5 47 2249207.5 48 2442177.5 49 2590870.0 50 2198718.5 51 1796070.5 52 1520132.5 53 1329925.0 54 1108952.0 55 684972.0 56 400213.5 57 246742.5 58 137036.0 59 102167.0 60 82481.5 61 93680.0 62 132129.0 63 130156.0 64 87102.0 65 39838.5 66 18501.5 67 11471.0 68 1486.0 69 791.5 70 430.0 71 279.5 72 164.0 73 123.5 74 66.0 75 43.0 76 42.5 77 39.0 78 31.5 79 26.5 80 31.5 81 26.0 82 21.0 83 23.5 84 18.0 85 13.0 86 10.5 87 11.0 88 13.0 89 11.0 90 8.5 91 7.5 92 8.5 93 8.0 94 6.0 95 4.5 96 4.0 97 4.5 98 4.5 99 7.5 100 15.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04682515271931453 2 0.05693651416130709 3 0.07183604207315146 4 0.06729168606003894 5 0.07282821518182656 6 0.0 7 9.346067508338741E-4 8 0.001408682638938013 9 0.0 10-14 0.0 15-19 4.74075888104139E-6 20-24 1.2258247963835596E-4 25-29 2.1130239584070198E-4 30-34 3.6977919272122845E-4 35-39 0.0012210840375025181 40-44 8.127015224642383E-5 45-49 0.0011709674436172234 50-54 9.657603091950033E-4 55-59 0.0019552244127952136 60-64 0.0024164325267936686 65-69 0.004175931322928745 70-74 0.00497779682509346 75-79 0.006767771928320945 80-84 0.00528323714728627 85-89 0.006781994204964069 90-94 0.003110615077231872 95-99 0.004429223297430099 100-104 0.0028451325798935544 105-109 0.007801257364387968 110-114 0.010692443030554496 115-119 0.01074188237317107 120-124 0.009158468906903246 125-129 0.006410860509705399 130-134 0.008618699645733249 135-139 0.005086157028088692 140-144 0.0029379160037082217 145-149 0.003928057358577152 150-151 0.002732708869286001 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 2.9531137E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 7.825636648092975 #Duplication Level Percentage of deduplicated Percentage of total 1 79.99609534911312 6.260203754683595 2 9.625933581926056 1.5065811722165885 3 3.360735077695534 0.7889967476563728 4 1.8420260687773444 0.5766010684226646 5 0.9666578159832977 0.3782356415462204 6 0.8058391321480763 0.3783722547003254 7 0.4145812222202302 0.2271053404352468 8 0.34776916255015966 0.2177212082823306 9 0.2823493430362378 0.19886070297864214 >10 1.574142425625003 2.308431687467713 >50 0.23399241005233484 1.2987494312799401 >100 0.2955271417181467 5.178436945710764 >500 0.08014841245077413 4.496591883401435 >1k 0.12354949512532974 21.707042733311564 >5k 0.027941899806712817 15.371973468973998 >10k+ 0.022711461772474753 39.10609595893261 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 201738 0.6831365822453772 Illumina Single End PCR Primer 1 (100% over 50bp) GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG 131552 0.4454687945133979 No Hit CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC 117759 0.3987621607661094 No Hit CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG 104477 0.35378590401040094 No Hit GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA 99734 0.33772489017270146 No Hit GTCAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTGACTTTCGTTC 84665 0.28669739333097805 No Hit GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA 79718 0.26994558319918394 No Hit CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG 79657 0.2697390215622243 No Hit GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT 76979 0.26067062707406086 No Hit ATCAAACCGTACTCACTTCACATTACAGCCCGACCGGCCCAGTCCTTAGA 76603 0.2593973946888669 No Hit GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC 74365 0.25181895299188783 No Hit CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA 73343 0.24835819900872763 No Hit CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG 68402 0.23162670641499514 No Hit CTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTGGATC 67845 0.22974056163160939 No Hit GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG 65525 0.22188444691445508 No Hit GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC 65149 0.22061121452926108 No Hit GTCGATCCTAAGATATTGGCTAGTGCCGTTTTTTAAAATAAAATAGAGGA 64085 0.21700823777966965 No Hit CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT 63866 0.216266647640421 No Hit CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC 63672 0.21560971390976244 No Hit GTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAATTAAAA 63038 0.2134628273879194 No Hit ATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAG 62076 0.21020524878537522 No Hit CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG 60624 0.2052884045744666 No Hit CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA 60346 0.2043470253109455 No Hit CGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGT 59161 0.20033431154377834 No Hit CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC 58364 0.19763546523792835 No Hit GCCAGATCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTA 54159 0.18339625731308618 No Hit GAACAATCCAACGCTTGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCG 53708 0.18186905570212214 No Hit CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC 53137 0.17993550332992597 No Hit CGGAGATAGGGATGTTGTTTCCGTAAAGCGCCACGGTTCCTGTGGTGTCT 52525 0.17786311444764216 No Hit CTCACTTCACATTACAGCCCGACCGGCCCAGTCCTTAGAGCCAATCCTTA 52512 0.17781909311517533 No Hit CTGGAATCGGTTCAGCCGGAGATAGGGATGTTGTTTCCGTAAAGCGCCAC 50283 0.17027112772528874 No Hit GTCGCAATGGTTAGCGTTGAAGACTTTGGGCGTAGGCCCGGTTGAAGCTT 49439 0.1674131273712895 No Hit CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG 49229 0.16670201353913328 No Hit CTCAGATGGTAGCTTCGCACCATTGGCCGATCGGCCAAGCACATGAACCA 48466 0.1641182999489657 No Hit CTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGA 48042 0.16268252725927893 No Hit CAACGGTTGAGTATAAGTCTCCCGCTCAAGCGCCATCCATTTTCAGGGCT 46561 0.15766748161440583 No Hit GGCATTCGTATCGCTGCGTGAGAGGTGAAATTCTTGGACCGTAGCGAGAC 46394 0.15710197680502447 No Hit AAACAGTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAAT 46262 0.15665499096766913 No Hit CGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCCACCAAAG 45820 0.1551582656637975 No Hit GTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTGGAT 45619 0.15447762813873372 No Hit GTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTAC 44679 0.15129454717574875 No Hit CTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCT 44457 0.15054279826746936 No Hit GTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCTAAGG 43878 0.1485821558445244 No Hit CACAGACCTGTTATTGCTCAACTTCATGCGGCTAAACACCGCTTGTCCCT 43866 0.14854152076840116 No Hit CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA 43746 0.14813517000716903 No Hit CACGCTTTCGCGGTCTGTATTCGTACTGGAAATCAAGATCAAGCGAGCTT 43211 0.14632352286334252 No Hit GGGAGTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCT 42957 0.14546341375206787 No Hit CCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTC 42658 0.14445092310533114 No Hit CTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAAC 42299 0.1432352570779784 No Hit GTCAATTTCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGAACCGA 41329 0.13995058842468544 No Hit CAGAAATCAGAGACAGTCAACGCCCGCAAGGGCCATCTGTCTCCTCTGTT 41165 0.13939524238433487 No Hit GTTTCCGTAAAGCGCCACGGTTCCTGTGGTGTCTCGTGCGCTTTGTTCGA 40218 0.13618845762694473 No Hit GACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTG 39363 0.1332932084531659 No Hit CGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGGTA 38329 0.12979181939388246 No Hit CTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTGC 37985 0.12862694721168372 No Hit ATTGCTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGT 37904 0.12835266044785204 No Hit GTTTACGGTCAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTGACT 37851 0.1281731888616412 No Hit CCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTT 37666 0.12754673143807502 No Hit CTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTT 36992 0.12526439466248793 No Hit CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT 36790 0.12458037088108054 No Hit GTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAATCACCG 36467 0.12348661008209742 No Hit GAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGC 36407 0.12328343470148136 No Hit TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT 36071 0.1221456525700314 No Hit ATTTTTTCAAGGTAAACTTGCTAGCCACCATATGCAACCAATTAAGGTAA 36009 0.12193570467672815 No Hit AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG 35326 0.11962289159404868 No Hit CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT 35306 0.11955516646717666 No Hit CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG 35046 0.11867473981784041 No Hit GGGCATTCGTATCGCTGCGTGAGAGGTGAAATTCTTGGACCGTAGCGAGA 34803 0.11785187952634535 No Hit GATAAATCGAAAGGGAAACGGGTTAATATTCCCGTACCTGGACACGGAGA 34508 0.11685293390498307 No Hit CTCGGATTTTCATGGATCGAACAAAGCGCACGAGACACCACAGGAACCGT 33996 0.11511917065705937 No Hit CTAGAGTCTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAAC 33882 0.11473313743388885 No Hit GTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTG 33728 0.11421165395697429 No Hit CTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTTCATGTGCTTGGC 33248 0.11258625091204581 No Hit GTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATTTTCATGGATC 33202 0.11243048312024016 No Hit GTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAA 33113 0.11212910630565968 No Hit GTTCGATTAGTCTTTCGCCCCTATACCAAAGTCAGACGATCGATTTGCAC 33086 0.11203767738438244 No Hit CTCACGTTGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTGCCC 32845 0.11122158960557463 No Hit CTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTTCGCACCA 32809 0.11109968437720498 No Hit GTTGAAGCTTCCATTGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAG 32652 0.11056804213125963 No Hit CTTGGACCGTAGCGAGACGTACGACTGCGAAAGCATTTGCCAAGAATGTC 32330 0.10947766758862011 No Hit CGAGACTCTAGCCTACTAAATAGTTACTGGATAATTTTATCGTCCAGACA 32316 0.1094302599998097 No Hit CTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAA 32277 0.10929819600240925 No Hit CTGACTTTCGTTCTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCA 32255 0.10922369836285004 No Hit GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT 32126 0.1087868712945255 No Hit CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCC 31680 0.10727660096527945 No Hit CTCACGTTCCCTATTGGTGGGTGAACAATCCAACGCTTGGCGAATTCTGC 31515 0.10671786866858529 No Hit CCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTG 31130 0.10541415997629892 No Hit GCTGATTCCTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCAT 31119 0.1053769111565193 No Hit TGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGC 31106 0.1053328898240525 No Hit GGTAATTCCAGCTCTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGC 30944 0.10478431629638912 No Hit GTGTAATCCATCTGATAAATCGAAAGGGAAACGGGTTAATATTCCCGTAC 30507 0.10330452227423549 No Hit CCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGG 30031 0.10169266425468142 No Hit GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC 29929 0.10134726610763413 No Hit CTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAACCGAGATC 29923 0.10132694856957251 No Hit CTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATTC 29771 0.10081223760534516 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0010836020299523179 0.0 0.0 2 0.0011208508497319288 0.0 0.0 3 0.0012021210019783526 0.0 0.0 4 0.0013748200755020032 0.0 0.0 5 0.001449317715061225 0.0 0.0 6 0.001493339047528038 0.0 0.0 7 0.0015644504307436588 0.0 0.0 8 0.0016728106337388906 0.0 0.0 9 0.0032203297827645442 0.0 0.0 10-11 0.003916205461374549 0.0 0.0 12-13 0.004241286070360244 0.0 0.0 14-15 0.005470497123087404 0.0 0.0 16-17 0.006667538740550355 0.0 0.0 18-19 0.007266906113367731 0.0 0.0 20-21 0.007917067331339123 0.0 0.0 22-23 0.008506275935125695 0.0 0.0 24-25 0.009488290274769981 0.0 0.0 26-27 0.011371048801812136 1.6931281718004965E-6 0.0 28-29 0.01343497204323694 5.079384515401489E-6 0.0 30-31 0.026064015076696846 6.772512687201986E-6 0.0 32-33 0.030462762067034535 6.772512687201986E-6 0.0 34-35 0.033820235231714915 6.772512687201986E-6 0.0 36-37 0.03788035658769251 6.772512687201986E-6 0.0 38-39 0.04286492592547317 6.772512687201986E-6 0.0 40-41 0.04891447288331635 6.772512687201986E-6 0.0 42-43 0.056150902689591664 6.772512687201986E-6 0.0 44-45 0.06442691319335249 6.772512687201986E-6 0.0 46-47 0.07454335401986045 6.772512687201986E-6 0.0 48-49 0.08651038393814636 6.772512687201986E-6 0.0 50-51 0.1006124484810727 6.772512687201986E-6 0.0 52-53 0.1180228854716972 6.772512687201986E-6 0.0 54-55 0.13892116649623074 6.772512687201986E-6 0.0 56-57 0.16249628316038084 6.772512687201986E-6 0.0 58-59 0.18639478730534487 6.772512687201986E-6 0.0 60-61 0.2161430492838796 6.772512687201986E-6 0.0 62-63 0.2501681530243824 6.772512687201986E-6 0.0 64-65 0.2881213141234623 6.772512687201986E-6 0.0 66-67 0.3318141797249459 6.772512687201986E-6 0.0 68-69 0.38323617543069877 1.0158769030802978E-5 8.465640859002482E-6 70-71 0.4432846591717752 1.0158769030802978E-5 1.1851897202603475E-5 72-73 0.5190572242443628 1.0158769030802978E-5 1.3545025374403972E-5 74-75 0.6125314443531247 1.0158769030802978E-5 1.3545025374403972E-5 76-77 0.7102655749421365 1.0158769030802978E-5 1.3545025374403972E-5 78-79 0.8254253806753191 1.1851897202603475E-5 1.3545025374403972E-5 80-81 0.972084142916678 1.3545025374403972E-5 1.3545025374403972E-5 82-83 1.1347615907914417 1.3545025374403972E-5 1.3545025374403972E-5 84-85 1.3181239855410918 1.3545025374403972E-5 1.3545025374403972E-5 86-87 1.541891190982589 1.3545025374403972E-5 1.3545025374403972E-5 88-89 1.802824252923279 1.3545025374403972E-5 1.3545025374403972E-5 90-91 2.094656903999328 1.6931281718004967E-5 1.6931281718004967E-5 92-93 2.4400042572014753 1.8624409889805464E-5 1.8624409889805464E-5 94-95 2.8498225449294416 2.370379440520695E-5 2.0317538061605957E-5 96-97 3.308255621854316 2.370379440520695E-5 2.0317538061605957E-5 98-99 3.841960097912925 2.370379440520695E-5 2.0317538061605957E-5 100-101 4.410879946816813 2.370379440520695E-5 2.0317538061605957E-5 102-103 5.056029505399673 2.370379440520695E-5 2.0317538061605957E-5 104-105 5.80159680272385 2.370379440520695E-5 2.0317538061605957E-5 106-107 6.644134629831557 2.370379440520695E-5 2.2010666233406454E-5 108-109 7.5311627859096655 2.370379440520695E-5 2.370379440520695E-5 110-111 8.49245831611563 2.370379440520695E-5 2.370379440520695E-5 112-113 9.64918485867984 2.370379440520695E-5 2.370379440520695E-5 114-115 10.832027903294073 2.370379440520695E-5 2.5396922577007447E-5 116-117 12.118481926381635 2.370379440520695E-5 2.7090050748807944E-5 118-119 13.48099465320282 2.370379440520695E-5 2.7090050748807944E-5 120-121 14.788634789104124 2.370379440520695E-5 2.7090050748807944E-5 122-123 16.23878044384136 2.370379440520695E-5 2.7090050748807944E-5 124-125 17.796026614214007 2.370379440520695E-5 3.216943526420943E-5 126-127 19.249504345193344 2.370379440520695E-5 3.724881977961092E-5 128-129 20.8206375528311 2.370379440520695E-5 3.724881977961092E-5 130-131 22.363592028305582 2.370379440520695E-5 3.724881977961092E-5 132-133 23.902628605190515 2.7090050748807944E-5 3.724881977961092E-5 134-135 25.43061074824176 2.7090050748807944E-5 4.0635076123211914E-5 136-137 26.988178612967054 2.7090050748807944E-5 4.0635076123211914E-5 138-139 28.45073828345993 2.7090050748807944E-5 4.0635076123211914E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAATC 10775 0.0 110.15088 2 GTCCAAT 11290 0.0 106.34665 1 CTTGTTA 18425 0.0 97.608765 1 ATCAAAG 14785 0.0 84.675735 6 CCAGACG 5690 0.0 83.066 6 TCAAAGG 15365 0.0 82.13991 7 GTTACGA 22020 0.0 81.77054 4 CAAAGGA 14825 0.0 81.07329 8 TTACGAC 22425 0.0 80.22904 5 CAGACGT 5920 0.0 79.961235 7 CCAATCA 16420 0.0 73.210144 3 CAATCAA 18245 0.0 66.521866 4 CGCGGAT 1945 0.0 65.27369 1 GCGAGCG 3095 0.0 60.006603 1 CCTGGCC 16690 0.0 59.853558 5 CAAACCG 20425 0.0 56.581657 3 TGTTACG 31940 0.0 56.034588 3 TCAAACC 21155 0.0 55.177876 2 ACCTGGC 18025 0.0 55.0986 4 CCGGCAT 1945 0.0 54.083916 1 >>END_MODULE