FastQCFastQC Report
Wed 5 Aug 2015
H55MKBCXX l02n01 6_f30b.3410000000ed4b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55MKBCXX l02n01 6_f30b.3410000000ed4b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25062942
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC3028221.2082460231524297TruSeq Adapter, Index 6 (100% over 50bp)
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG1074310.4286448095359276No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA813860.3247264427296684No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC760210.3033203364553132No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA712300.2842044641048126No Hit
GAACAATCCAACGCTTGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCG685930.27368295390062347No Hit
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG683750.27281314380410726No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC592310.23632899920528086No Hit
GTCAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTGACTTTCGTTC572010.2282293914257951No Hit
GCCAGATCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTA566310.22595511732022522No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG564430.22520500586084424No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT561400.22399604962577818No Hit
GTCGATCCTAAGATATTGGCTAGTGCCGTTTTTTAAAATAAAATAGAGGA558220.22272724407214445No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG534040.21307953391904272No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG512240.20438143295388064No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA512010.20428966399874365No Hit
ATCAAACCGTACTCACTTCACATTACAGCCCGACCGGCCCAGTCCTTAGA506400.2020512994843143No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC499070.19912666278364288No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT484090.19314971083602236No Hit
CTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAAC472880.18867697176173492No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA467640.1865862355664391No Hit
CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG467390.1864864867021597No Hit
CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC466340.1860675414721863No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC461070.18396483541317696No Hit
GTCGCAATGGTTAGCGTTGAAGACTTTGGGCGTAGGCCCGGTTGAAGCTT460320.18366558882033882No Hit
GGGAGTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCT448870.17909709083634315No Hit
GTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAATTAAAA433250.17286478179616743No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT427220.17045883918974875No Hit
CAGAAATCAGAGACAGTCAACGCCCGCAAGGGCCATCTGTCTCCTCTGTT401570.16022460571468425No Hit
GTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCTAAGG390600.155847625550105No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC388520.15501771499930056No Hit
ATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAG381160.1520811084349156No Hit
GAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGC379850.15155842438609163No Hit
CGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGT372730.14871757673141486No Hit
CACGCTTTCGCGGTCTGTATTCGTACTGGAAATCAAGATCAAGCGAGCTT370380.14777993740718867No Hit
CAACGGTTGAGTATAAGTCTCCCGCTCAAGCGCCATCCATTTTCAGGGCT370250.1477280679977634No Hit
CTCAGATGGTAGCTTCGCACCATTGGCCGATCGGCCAAGCACATGAACCA367370.14657896108126492No Hit
CGGAGATAGGGATGTTGTTTCCGTAAAGCGCCACGGTTCCTGTGGTGTCT366640.14628769439756914No Hit
GCCGGAGATAGGGATGTTGTTTCCGTAAAGCGCCACGGTTCCTGTGGTGT366420.14619991539700328No Hit
GGGCATTCGTATCGCTGCGTGAGAGGTGAAATTCTTGGACCGTAGCGAGA354540.1414598493664471No Hit
GGCGAATTCTGCTTCGCAATGATAGGAAGAGCCGACATCGAAGGATCAAA354080.141276311456173No Hit
AAACAGTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAAT349140.13930527389801245No Hit
CTAGAGTCTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAAC344220.13734221624899423No Hit
GATAAATCGAAAGGGAAACGGGTTAATATTCCCGTACCTGGACACGGAGA341380.13620906915078046No Hit
GTTTCCGTAAAGCGCCACGGTTCCTGTGGTGTCTCGTGCGCTTTGTTCGA339860.1356025960559618No Hit
GGCATTCGTATCGCTGCGTGAGAGGTGAAATTCTTGGACCGTAGCGAGAC339620.1355068371462536No Hit
CTGGAATCGGTTCAGCCGGAGATAGGGATGTTGTTTCCGTAAAGCGCCAC338630.13511183164370727No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT327720.13075879120655506No Hit
CTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTT323820.12920270892379673No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA322410.12864012532926103No Hit
GTTTACGGTCAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTGACT318130.126932424772798No Hit
CCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTC317880.12683267590851863No Hit
CGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGGTA317810.1268047462265204No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC313460.12506911598805917No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAATCACCG307450.12267115329078286No Hit
GCTGATTCCTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCAT307360.12263524369964228No Hit
CTCACTTCACATTACAGCCCGACCGGCCCAGTCCTTAGAGCCAATCCTTA307280.12260332406307288No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG302820.12082380432432871No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG302100.12053652759520411No Hit
GTTCGATTAGTCTTTCGCCCCTATACCAAAGTCAGACGATCGATTTGCAC300170.11976646636296728No Hit
CGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCCACCAAAG299920.11966671749868789No Hit
GCCGCAACGAGTAAGAGGGTCGCAATGGTTAGCGTTGAAGACTTTGGGCG295160.1177674991228085No Hit
GTTGAAGCTTCCATTGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAG294040.11732062421083685No Hit
GTGTAATCCATCTGATAAATCGAAAGGGAAACGGGTTAATATTCCCGTAC291330.11623934652204837No Hit
CTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCT285870.11406083132618669No Hit
GTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATTTTCATGGATC285420.1138812833704838No Hit
GGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTA284390.11347031804965275No Hit
CTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTGC283840.1132508705482381No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT280800.11203792435860085No Hit
CCCGGTTGAAGCTTCCATTGGTGCAGATCTTGGTGGTAGTAGCAAATATT278470.11110826494351701No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT277870.11086886766924649No Hit
TTTGAATTTCGTGCCAGATCGTACCCATATCCGCAGCAGGTCTCCAAGGT276790.11043795257555956No Hit
GTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTGGAT273910.1092888456590611No Hit
CTCGGATTTTCATGGATCGAACAAAGCGCACGAGACACCACAGGAACCGT272720.10881404106509124No Hit
CTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGA271750.10842701547168723No Hit
GTCAATTTCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGAACCGA271320.1082554474251267No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGC266950.10651183727752314No Hit
GCGGATATGGGTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATT265900.10609289204754974No Hit
ATTGCTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGT263170.10500363444961888No Hit
GTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGGCAACCA261310.10426150289938028No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT260320.10386649739683394No Hit
GTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTAC258980.10333184348429647No Hit
GCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAA257490.10273734025319134No Hit
GGAAAACTGGCAATATTAGCCTAGGAGCCTAGTTCTCATCCAATGGATGG257220.10262961147976962No Hit
CACAGACCTGTTATTGCTCAACTTCATGCGGCTAAACACCGCTTGTCCCT257120.10258971193405786No Hit
CTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTGGATC255680.10201515847580864No Hit
CTCACGTTCCCTATTGGTGGGTGAACAATCCAACGCTTGGCGAATTCTGC254030.1013568159715647No Hit
ATTTTTTCAAGGTAAACTTGCTAGCCACCATATGCAACCAATTAAGGTAA252040.10056281501190083No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG251030.10015982960021212No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCAAT69000.096.950751
TCCAATC72950.091.4369052
CTTGTTA130900.089.239191
TCCCTAC15200.080.122839
GCACGAC31200.075.282348
ATCAAAG94500.075.179076
GTTACGA155650.075.135344
CCAGACG42450.074.2872546
TTACGAC158850.073.575825
AAGCACG32500.072.49416
TCAAAGG102050.070.8247157
CAAAGGA98350.069.435138
CAGACGT45800.069.170147
CCGGCAT15650.067.223791
CTCCGAT28650.060.01961
CCAATCA114300.059.4998173
CCTGGCC120150.058.8956645
ACCTGGC123900.057.2303774
GCGAGCG29450.056.9109761
CAATCAA122500.056.641524
Produced by FastQC (version 0.11.2)