FastQCFastQC Report
Wed 5 Aug 2015
H55MKBCXX l02n01 5_f30a.3410000000ed3e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55MKBCXX l02n01 5_f30a.3410000000ed3e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20555798
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC7117743.4626434838482067TruSeq Adapter, Index 5 (100% over 50bp)
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA761370.3703918475945327No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG758080.3687913259314963No Hit
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG677720.3296977329705225No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC663860.3229551097943267No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG531840.25872992135844103No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT505050.24569710210228762No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT486780.23680909882457493No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC473520.23035836409756508No Hit
GTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAATTAAAA418530.20360678772967122No Hit
GTCAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTGACTTTCGTTC409270.19910197599723448No Hit
CCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTC402220.19567228671929934No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC400360.19476743252682285No Hit
CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC395260.19228638070874213No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC388780.18913398545753368No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA384380.18699347016350326No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG379820.1847751179496899No Hit
CTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAAC378120.18394810067699632No Hit
ATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAG377170.18348594396578524No Hit
AAACAGTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAAT357750.17403848782713277No Hit
GTCGCAATGGTTAGCGTTGAAGACTTTGGGCGTAGGCCCGGTTGAAGCTT354610.17251093827639286No Hit
ATCAAACCGTACTCACTTCACATTACAGCCCGACCGGCCCAGTCCTTAGA345350.16800612654395614No Hit
CGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGT342100.16642506411086547No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC339750.16528183435155375No Hit
CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG338710.16477589437296475No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG327750.15944406536783443No Hit
CTAGAGTCTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAAC318270.15483222787069614No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA313200.1523657704750747No Hit
GTCGATCCTAAGATATTGGCTAGTGCCGTTTTTTAAAATAAAATAGAGGA309950.15078470804198407No Hit
CGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGGTA301310.14658151437370615No Hit
GCCAGATCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTA300410.1461436816999272No Hit
CTCCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAG283210.1377762128232628No Hit
GGAATAATGAAATAGGATCTCGGTTCTATTTTGTTGGTTTTCTGATCTGA282410.1373870282243482No Hit
GGGCATTCGTATCGCTGCGTGAGAGGTGAAATTCTTGGACCGTAGCGAGA278310.13539245715491074No Hit
CTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGA274990.13377734106941505No Hit
CTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTGGATC272050.13234708766840383No Hit
CTCAGATGGTAGCTTCGCACCATTGGCCGATCGGCCAAGCACATGAACCA270870.13177304038500476No Hit
CAGAAATCAGAGACAGTCAACGCCCGCAAGGGCCATCTGTCTCCTCTGTT267500.1301336002620769No Hit
GTCAATTTCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGAACCGA264530.12868875243860636No Hit
CTGACTTTCGTTCTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCA262660.12777903343864344No Hit
GGGAGTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCT259100.12604716197347338No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA257590.125312576043022No Hit
GGAAAACTGGCAATATTAGCCTAGGAGCCTAGTTCTCATCCAATGGATGG255090.12409637417141384No Hit
CTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCT254880.12399421321419873No Hit
GTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTGGAT250220.12172721292552106No Hit
GTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTG248500.12089046603785464No Hit
ATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGGATCGACTGACCC248390.12083695315550386No Hit
GCTGATTCCTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCAT247960.12062776643358725No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG246290.11981534358335298No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT244020.11871103228393275No Hit
GGAAGTTGGAAACCGCTAAGGAGTGTGTAACAACTCACCTGCCAAATCAA243510.11846292710212467No Hit
GAACAATCCAACGCTTGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCG242170.11781104289894266No Hit
GTTTACGGTCAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTGACT241850.11765536905937682No Hit
CAACGGTTGAGTATAAGTCTCCCGCTCAAGCGCCATCCATTTTCAGGGCT241070.11727591407543507No Hit
ATTTTTTCAAGGTAAACTTGCTAGCCACCATATGCAACCAATTAAGGTAA238160.11586025509688312No Hit
CTGGAATCGGTTCAGCCGGAGATAGGGATGTTGTTTCCGTAAAGCGCCAC238040.11580187740704595No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGC236740.11516945243380967No Hit
GCCGGAGATAGGGATGTTGTTTCCGTAAAGCGCCACGGTTCCTGTGGTGT236690.11514512839637751No Hit
GTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCTAAGG235590.1146099995728699No Hit
CACAGACCTGTTATTGCTCAACTTCATGCGGCTAAACACCGCTTGTCCCT234770.1142110853589824No Hit
ATTGCTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGT231820.11277596715048475No Hit
CTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATTC231440.11259110446600029No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA228520.11117058067996193No Hit
GATAAATCGAAAGGGAAACGGGTTAATATTCCCGTACCTGGACACGGAGA227240.11054788532169854No Hit
GTCAGATAGGTGATTAACGTTCACATCGAGATGTAACTTTATTGCATACA226890.11037761705967339No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG226860.11036302263721408No Hit
CTCCATTCCTCTATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGG226590.1102316728350804No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT225440.10967221997414062No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAACTCGTATGC224390.10916141518806519TruSeq Adapter, Index 5 (98% over 50bp)
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT222590.10828574984050729No Hit
CTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAACCGAGATC221310.10766305448224389No Hit
GTTAATATTCCCGTACCTGGACACGGAGATAAATGCTCATCGTGAGAATT220380.10721062738600563No Hit
CCCGGTTGAAGCTTCCATTGGTGCAGATCTTGGTGGTAGTAGCAAATATT218540.10631550280850201No Hit
CCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTT218200.10615009935396329No Hit
GATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATA218080.10609172166412612No Hit
GTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAA217310.10571713148767078No Hit
CTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCAGTCGTACGTCTC216500.10532308208126971No Hit
GGCATTCGTATCGCTGCGTGAGAGGTGAAATTCTTGGACCGTAGCGAGAC215750.10495822151978726No Hit
GTTGAAGCTTCCATTGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAG213990.1041020154021751No Hit
GTGAAATTCTTGGACCGTAGCGAGACGTACGACTGCGAAAGCATTTGCCA213830.10402417848239216No Hit
CGGAGATAGGGATGTTGTTTCCGTAAAGCGCCACGGTTCCTGTGGTGTCT213180.10370796599577403No Hit
GTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTAC213000.10362039946101825No Hit
GAAATTCTTGGACCGTAGCGAGACGTACGACTGCGAAAGCATTTGCCAAG212570.10341121273910164No Hit
TTTGAATTTCGTGCCAGATCGTACCCATATCCGCAGCAGGTCTCCAAGGT212440.10334797024177801No Hit
GCCGCAACGAGTAAGAGGGTCGCAATGGTTAGCGTTGAAGACTTTGGGCG212210.10323607966959006No Hit
CTCACTTCACATTACAGCCCGACCGGCCCAGTCCTTAGAGCCAATCCTTA210260.10228744220973567No Hit
CGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCCACCAAAG210080.10219987567497987No Hit
CTCGGATTTTCATGGATCGAACAAAGCGCACGAGACACCACAGGAACCGT207070.1007355686215636No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT206480.10044854497986407No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTTA112900.091.388911
GTCCAAT46250.085.815051
GCACGAC27350.080.315038
CCAGACG43600.080.310486
GTTACGA131350.078.880324
TTACGAC133150.077.705065
TCCAATC51450.077.225682
TCCCTAC18800.074.420779
CAGACGT47350.073.950097
AAGCACG32150.068.3246
CCTGGCC124200.065.032455
ATCAAAG69850.064.140696
ACCTGGC125250.064.139944
CGGGCAT6100.063.042561
CACCTGG129350.062.443223
CTCCGAT26500.061.0585941
CACTCTT24050.060.8979642
TCAAAGG73700.060.8884247
GCGAGCG23100.059.9941941
TGGCTCA33950.059.5841753
Produced by FastQC (version 0.11.2)