##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H55MKBCXX l01n02 8_m30b.3420000000ed61.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 20825355 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.05160997255509 35.0 35.0 35.0 32.0 35.0 2 34.11417985431701 35.0 35.0 35.0 32.0 35.0 3 34.052174716829555 35.0 35.0 35.0 32.0 35.0 4 33.98425424200452 35.0 35.0 35.0 32.0 35.0 5 34.08562115747847 35.0 35.0 35.0 32.0 35.0 6 38.64375214732234 40.0 39.0 40.0 36.0 40.0 7 38.70704600233705 40.0 39.0 40.0 36.0 40.0 8 38.64307278315304 40.0 39.0 40.0 36.0 40.0 9 38.6911005838796 40.0 39.0 40.0 36.0 40.0 10-14 38.70414252242038 40.0 39.0 40.0 36.2 40.0 15-19 38.682820869080025 40.0 39.0 40.0 36.0 40.0 20-24 38.66260496399701 40.0 39.0 40.0 36.0 40.0 25-29 38.644529862756244 40.0 39.0 40.0 36.0 40.0 30-34 38.62786937365534 40.0 39.0 40.0 36.0 40.0 35-39 38.6016417007057 40.0 39.0 40.0 36.0 40.0 40-44 38.531701822129804 40.0 39.0 40.0 36.0 40.0 45-49 38.460275284623 40.0 39.0 40.0 36.0 40.0 50-54 38.41745046843139 40.0 39.0 40.0 36.0 40.0 55-59 38.40782675733499 40.0 39.0 40.0 36.0 40.0 60-64 38.399832098900596 40.0 39.0 40.0 36.0 40.0 65-69 38.37226106349688 40.0 39.0 40.0 36.0 40.0 70-74 38.30677927939284 40.0 39.0 40.0 35.8 40.0 75-79 38.25500602510737 40.0 39.0 40.0 35.0 40.0 80-84 38.161519724393656 40.0 39.0 40.0 35.0 40.0 85-89 38.19816582238334 40.0 39.0 40.0 35.0 40.0 90-94 38.17112638896192 40.0 39.0 40.0 35.0 40.0 95-99 38.11646035325689 40.0 39.0 40.0 34.6 40.0 100-104 37.105002704635766 39.0 37.8 39.4 32.0 39.6 105-109 38.080555870476154 40.0 39.0 40.0 34.6 40.0 110-114 38.11174876970885 40.0 39.0 40.0 34.4 40.0 115-119 38.02978262795521 40.0 39.0 40.0 34.0 40.0 120-124 37.95911315797498 40.0 39.0 40.0 34.0 40.0 125-129 37.89474085795896 40.0 39.0 40.0 34.0 40.0 130-134 37.77495598994591 40.0 39.0 40.0 34.0 40.0 135-139 37.689922395080416 40.0 39.0 40.0 34.0 40.0 140-144 37.57163400095701 40.0 39.0 40.0 34.0 40.0 145-149 37.40296833355302 40.0 39.0 40.0 32.2 40.0 150-151 35.58571030361787 38.5 35.0 39.5 23.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.30103332652161185 2201 2 0.3241834083561841 2201 3 0.3220566491562238 2201 4 0.3469881850921084 2201 5 0.3011515035113419 2201 6 0.48074300328269004 2201 7 0.4557132503874328 2201 8 0.5445224197638723 2201 9 0.5055046375394454 2201 10-14 0.4860589307261094 2201 15-19 0.47903173366756846 2201 20-24 0.4731557829219284 2201 25-29 0.4937490212080675 2201 30-34 0.4772417898032941 2201 35-39 0.48273595387750134 2201 40-44 0.490350056614254 2201 45-49 0.5117423151143257 2201 50-54 0.5289105549437991 2201 55-59 0.5300522128855221 2201 60-64 0.5097316973641668 2201 65-69 0.5028809067580085 2201 70-74 0.5149776665061125 2201 75-79 0.502259284285671 2201 80-84 0.5816949260244186 2201 85-89 0.533651785556728 2201 90-94 0.5267574797319341 2201 95-99 0.5262165887990307 2201 100-104 0.5418359351048636 2201 105-109 0.5531888187052658 2201 110-114 0.5039313875814244 2201 115-119 0.5073648173258221 2201 120-124 0.5177312105337251 2201 125-129 0.5487719183109476 2201 130-134 0.5416311141721621 2201 135-139 0.542213198636027 2201 140-144 0.5550722961873262 2201 145-149 0.560156099037016 2201 150-151 0.5915043468801429 1101 1 0.33448935000991753 1101 2 0.3192162123225515 1101 3 0.3311775029513484 1101 4 0.27036865415493594 1101 5 0.29396000828023006 1101 6 0.44248742675159036 1101 7 0.47540815927513336 1101 8 0.5761578557469136 1101 9 0.49259894995213216 1101 10-14 0.4943762483744081 1101 15-19 0.47172418467557975 1101 20-24 0.4674814687881579 1101 25-29 0.46474558076803163 1101 30-34 0.49849408429459174 1101 35-39 0.4660920288810999 1101 40-44 0.461457422399036 1101 45-49 0.50019083102287 1101 50-54 0.49919302355377226 1101 55-59 0.4997463001952269 1101 60-64 0.464186836693699 1101 65-69 0.4955548077084728 1101 70-74 0.48378606573744065 1101 75-79 0.5183800530592109 1101 80-84 0.5618405371101503 1101 85-89 0.5240119655865314 1101 90-94 0.506711699527898 1101 95-99 0.5285209812883522 1101 100-104 0.6387148142734205 1101 105-109 0.529914164194075 1101 110-114 0.49281117677671915 1101 115-119 0.5174749496219562 1101 120-124 0.5197560505454959 1101 125-129 0.5361024484827439 1101 130-134 0.5554265108753142 1101 135-139 0.5580289565771821 1101 140-144 0.5369525495037664 1101 145-149 0.5597210241914965 1101 150-151 0.5938571526136158 2202 1 0.24984283230195103 2202 2 0.2300171933206343 2202 3 0.25777057654043034 2202 4 0.2650349183658065 2202 5 0.2879631874878328 2202 6 0.4441485777628813 2202 7 0.40216060597455083 2202 8 0.4791283634998251 2202 9 0.46503739630063734 2202 10-14 0.39880843663625143 2202 15-19 0.3945201749759022 2202 20-24 0.40309991768505427 2202 25-29 0.41295065908752804 2202 30-34 0.398679551609618 2202 35-39 0.3862603397944042 2202 40-44 0.4148402475649675 2202 45-49 0.42498373159898506 2202 50-54 0.44407577350622773 2202 55-59 0.4274948809743293 2202 60-64 0.4074412312159481 2202 65-69 0.3634453158460218 2202 70-74 0.39026930437115936 2202 75-79 0.43234110569143525 2202 80-84 0.4667089558865243 2202 85-89 0.405993958960714 2202 90-94 0.4054497354031028 2202 95-99 0.4381853407935026 2202 100-104 0.34072829851844944 2202 105-109 0.395201481233876 2202 110-114 0.3801222649212903 2202 115-119 0.37116664402496014 2202 120-124 0.3805662784550705 2202 125-129 0.3976837397239237 2202 130-134 0.45267228030353834 2202 135-139 0.4275971062710724 2202 140-144 0.4379068430651927 2202 145-149 0.4989556225075873 2202 150-151 0.5989090464039251 1102 1 0.22748342559705748 1102 2 0.2382461686341557 1102 3 0.24825736969454937 1102 4 0.20266312343085247 1102 5 0.25932789551116286 1102 6 0.37964258722418975 1102 7 0.33626074455753496 1102 8 0.4546870869616271 1102 9 0.4086236628739002 1102 10-14 0.3888716019540368 1102 15-19 0.361754589336968 1102 20-24 0.3823687317270341 1102 25-29 0.37852400913391193 1102 30-34 0.37171524516411836 1102 35-39 0.361117485360964 1102 40-44 0.3921311310641329 1102 45-49 0.3556027352514519 1102 50-54 0.34772706237969686 1102 55-59 0.3209133879563595 1102 60-64 0.3606379678613152 1102 65-69 0.35544438380381393 1102 70-74 0.33445288421290087 1102 75-79 0.340082463528816 1102 80-84 0.4157356247158077 1102 85-89 0.34688167825378 1102 90-94 0.3519622641127498 1102 95-99 0.3700557960409512 1102 100-104 0.3826143731905347 1102 105-109 0.3675459050237464 1102 110-114 0.3829826028473491 1102 115-119 0.39222685372953947 1102 120-124 0.3864475616321954 1102 125-129 0.3981330075469742 1102 130-134 0.392662234699813 1102 135-139 0.39492459861776297 1102 140-144 0.44383308627664775 1102 145-149 0.4211968233625214 1102 150-151 0.719906250687842 2203 1 0.10703835830448583 2203 2 0.16673150012616134 2203 3 0.19547874146967104 2203 4 0.1737033684273115 2203 5 0.19997050407519623 2203 6 0.31260783410464654 2203 7 0.2651054875093095 2203 8 0.3102724362921023 2203 9 0.31078471024803633 2203 10-14 0.291277722811472 2203 15-19 0.290825961014491 2203 20-24 0.30476677156611487 2203 25-29 0.2784867907546982 2203 30-34 0.2933910974767784 2203 35-39 0.28793292563516104 2203 40-44 0.3034881221128387 2203 45-49 0.2734404110768054 2203 50-54 0.2910077472601884 2203 55-59 0.2693681944155486 2203 60-64 0.2903074601919471 2203 65-69 0.2792189935495699 2203 70-74 0.2932208059490975 2203 75-79 0.2706958030901845 2203 80-84 0.34736399328082257 2203 85-89 0.23433343126733064 2203 90-94 0.24860090261375944 2203 95-99 0.24027158157824147 2203 100-104 0.29058078720205316 2203 105-109 0.2817855155547804 2203 110-114 0.2926715864349987 2203 115-119 0.31976382909066103 2203 120-124 0.2841913297316765 2203 125-129 0.3001792779783443 2203 130-134 0.31128443126421956 2203 135-139 0.2850943688529952 2203 140-144 0.32893810052266304 2203 145-149 0.34357765649245664 2203 150-151 0.6525896191899179 1103 1 0.11267650376293403 1103 2 0.15504792747235996 1103 3 0.09440476267860731 1103 4 0.12038693790165667 1103 5 0.1172776521544705 1103 6 0.1402097082852336 1103 7 0.16649108289742287 1103 8 0.22641124875337226 1103 9 0.2219084156186213 1103 10-14 0.20782493049624406 1103 15-19 0.2260457787595982 1103 20-24 0.2012571335278679 1103 25-29 0.18124740543773044 1103 30-34 0.21335052868185755 1103 35-39 0.15673295549770927 1103 40-44 0.20493619316372502 1103 45-49 0.22538589066014225 1103 50-54 0.15673699351054182 1103 55-59 0.18939613684073464 1103 60-64 0.17623782918043673 1103 65-69 0.20737029286327413 1103 70-74 0.20841076412379778 1103 75-79 0.21811541426255587 1103 80-84 0.26494340709736974 1103 85-89 0.21269757361117314 1103 90-94 0.24404545036576764 1103 95-99 0.21149048315252372 1103 100-104 0.2367313483675062 1103 105-109 0.21823492150426915 1103 110-114 0.20128812317753386 1103 115-119 0.23891828660682535 1103 120-124 0.16579444357596884 1103 125-129 0.22542469928561104 1103 130-134 0.24515230310492342 1103 135-139 0.25890662805898756 1103 140-144 0.2655488537914792 1103 145-149 0.24979260830114214 1103 150-151 0.4969886990901031 2204 1 -0.020876521957376326 2204 2 -0.047303313204153596 2204 3 0.02248358538540174 2204 4 0.032152101463474025 2204 5 0.0803023014542461 2204 6 0.038757381945011105 2204 7 0.06100552146428129 2204 8 0.12091299936869149 2204 9 0.05093423786577489 2204 10-14 0.02795477611910968 2204 15-19 0.0764081175578113 2204 20-24 0.08070208580984684 2204 25-29 0.021013642196493265 2204 30-34 -7.283197799097252E-4 2204 35-39 0.0695521996556181 2204 40-44 0.03591814819364458 2204 45-49 0.021821580588898826 2204 50-54 0.1356474980870246 2204 55-59 0.17097547222498122 2204 60-64 0.10575576669062059 2204 65-69 0.11814569110056539 2204 70-74 0.08180277696074256 2204 75-79 0.12130230440508427 2204 80-84 0.16966945581961568 2204 85-89 0.10381538810745639 2204 90-94 0.062497660834473834 2204 95-99 0.09612297969467676 2204 100-104 0.20806608621464306 2204 105-109 0.11835634461895239 2204 110-114 0.10151723514625388 2204 115-119 0.16365227065094246 2204 120-124 0.21878406816352225 2204 125-129 0.2224305425945161 2204 130-134 0.166927304578266 2204 135-139 0.13254308437274887 2204 140-144 0.13360216365750688 2204 145-149 0.2530823843556078 2204 150-151 0.46675244745102873 2205 1 -0.027827017081726524 2205 2 -0.014182629470624875 2205 3 0.014503010354495416 2205 4 0.0631133742111416 2205 5 0.09419207746017122 2205 6 0.11903488510600368 2205 7 0.07952396504634862 2205 8 0.1542200695520748 2205 9 0.1315882375146984 2205 10-14 0.14826472433183824 2205 15-19 0.15453744944145598 2205 20-24 0.16309451358459626 2205 25-29 0.16160157590791613 2205 30-34 0.11630118467957828 2205 35-39 0.07526929867471921 2205 40-44 0.16880074929846955 2205 45-49 0.17140651401056317 2205 50-54 0.16251595317354628 2205 55-59 0.10428986629337089 2205 60-64 0.1625830130633048 2205 65-69 0.14991461541396944 2205 70-74 0.1349568419536098 2205 75-79 0.11280505143545128 2205 80-84 0.15334859802546674 2205 85-89 0.0239097429428341 2205 90-94 0.020430913220494062 2205 95-99 0.12350432846940151 2205 100-104 0.21100464804720787 2205 105-109 0.022904863330154512 2205 110-114 0.04405762742688779 2205 115-119 0.18208812324490964 2205 120-124 0.002201175140868372 2205 125-129 0.09550140316478206 2205 130-134 0.14058177916636794 2205 135-139 0.08215266842466207 2205 140-144 0.12245963862252296 2205 145-149 0.02618484656471054 2205 150-151 0.35587586218444756 1216 1 -0.00307000146002423 1216 2 0.05958073426629085 1216 3 0.05562685201075368 1216 4 -0.005062521164788336 1216 5 0.05697086709284349 1216 6 0.036174789030283705 1216 7 0.0344342310496657 1216 8 0.17283956192534333 1216 9 0.11897250001555193 1216 10-14 0.049590002673014055 1216 15-19 0.05125450759098982 1216 20-24 0.12234227240617912 1216 25-29 0.10711478605427516 1216 30-34 0.0949724315858731 1216 35-39 0.07229823085713605 1216 40-44 0.09147640687388758 1216 45-49 0.0443618293702599 1216 50-54 0.09407136868023258 1216 55-59 0.051086575004148926 1216 60-64 0.04084921575876166 1216 65-69 0.07203136996053416 1216 70-74 0.09290003645649847 1216 75-79 0.07478289409642258 1216 80-84 0.1421477792081447 1216 85-89 0.07839004960432305 1216 90-94 0.0953520981713254 1216 95-99 0.09641698293436463 1216 100-104 -0.05029896230329456 1216 105-109 0.08329413672152697 1216 110-114 0.04906585729532509 1216 115-119 0.05315245092608478 1216 120-124 0.0073238746345367645 1216 125-129 0.0633469424919113 1216 130-134 0.041686587243553674 1216 135-139 0.083423482586376 1216 140-144 0.10164088412534511 1216 145-149 0.12270333482237561 1216 150-151 0.31475233649066325 2206 1 -0.020079132548154632 2206 2 0.024355669363444576 2206 3 0.01422514193745883 2206 4 -0.003161392002816399 2206 5 -0.02079807195064376 2206 6 -0.024560690189041168 2206 7 -0.05201566245160194 2206 8 -0.009648493029651206 2206 9 -0.031171151075611192 2206 10-14 -0.04269746711471356 2206 15-19 -0.046729977658358735 2206 20-24 -0.008866536855499874 2206 25-29 -0.06891990564500361 2206 30-34 -0.08683499620963886 2206 35-39 -0.06467899980331993 2206 40-44 -0.06470443735533848 2206 45-49 -0.1142356577788064 2206 50-54 -0.03916125826157213 2206 55-59 -0.15537948470610274 2206 60-64 -0.04348719325778916 2206 65-69 -0.038103034426164584 2206 70-74 -0.04094232408725418 2206 75-79 -0.024798681018161517 2206 80-84 0.008390477770731763 2206 85-89 -0.04500377211334694 2206 90-94 -0.04845482630231146 2206 95-99 -0.07717580605058316 2206 100-104 0.059312299892873455 2206 105-109 -0.014566951304622933 2206 110-114 -0.004088999344681099 2206 115-119 -0.03512094659718912 2206 120-124 0.004151323421837105 2206 125-129 -0.03491503238674909 2206 130-134 0.014827060666398495 2206 135-139 -0.05498574573191917 2206 140-144 -0.005882149662319591 2206 145-149 0.03667458166976445 2206 150-151 0.25994581635073644 2207 1 -0.1041217123268865 2207 2 -0.07435389025731354 2207 3 -0.09263702948745589 2207 4 -0.1008857487312298 2207 5 -0.12347204916520127 2207 6 -0.2268387799512297 2207 7 -0.24020669913537773 2207 8 -0.12140529860590021 2207 9 -0.14622130453388138 2207 10-14 -0.1540508274215 2207 15-19 -0.1807237767991694 2207 20-24 -0.19093554177253935 2207 25-29 -0.15972507639074252 2207 30-34 -0.16902872472564212 2207 35-39 -0.17807787377044804 2207 40-44 -0.18995026774052093 2207 45-49 -0.18555605091678018 2207 50-54 -0.16494070079473744 2207 55-59 -0.2902565880686012 2207 60-64 -0.20356597042583502 2207 65-69 -0.1447883860923298 2207 70-74 -0.19214592198210312 2207 75-79 -0.1983132052121377 2207 80-84 -0.13773982136702756 2207 85-89 -0.1895454648720616 2207 90-94 -0.20105786663305025 2207 95-99 -0.13828701487688733 2207 100-104 -0.07198835148858507 2207 105-109 -0.15866575864283305 2207 110-114 -0.16180912075468967 2207 115-119 -0.17022370281039656 2207 120-124 -0.13200104157028392 2207 125-129 -0.14277596304586382 2207 130-134 -0.16167132471889545 2207 135-139 -0.10312593123340008 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 0.0 13 1.0 14 3.0 15 13.0 16 146.0 17 1300.0 18 3906.0 19 11910.0 20 23517.0 21 37063.0 22 52270.0 23 68092.0 24 86293.0 25 104168.0 26 129368.0 27 174375.0 28 168799.0 29 184080.0 30 210469.0 31 246217.0 32 291614.0 33 353941.0 34 438179.0 35 551412.0 36 725754.0 37 1068569.0 38 2047202.0 39 1.3846693E7 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.034233750156965 13.226871762810285 7.38848869563088 45.350405791401876 2 19.997464629054342 17.496446038975087 47.56547967609676 14.940609655873812 3 21.23020712011872 22.317924472355934 25.758105924244752 30.693762483280594 4 25.142769474998143 35.561218524246044 19.45860226632391 19.837409734431898 5 25.49299639790054 32.754870205093745 22.25949569647192 19.492637700533795 6 17.998017320713142 36.35702248533098 27.65431369597301 17.99064649798287 7 15.006558111494378 17.104692813159726 45.39338705150524 22.495362023840652 8 18.258747569969398 18.758916714745077 32.872755350388985 30.109580364896544 9 25.184026874932027 19.383828030782666 31.665587453371142 23.766557640914165 10-14 23.18107326381711 27.551385318521582 26.428586691559396 22.83895472610191 15-19 23.25713727329018 26.32343890416274 27.510490937609468 22.908932884937617 20-24 23.506552469333656 26.777388428672644 26.49560979872852 23.220449303265177 25-29 24.10321168594725 26.73898716252376 26.10711894227013 23.05068220925886 30-34 22.889742133062523 26.817587197681597 26.740944079075152 23.551726590180728 35-39 22.30728850995762 27.54177143879523 26.52736275215298 23.623577299094173 40-44 23.018046030907996 27.166692716642764 27.551830929172638 22.2634303232766 45-49 22.911222104577867 26.71782363383966 27.013705230647016 23.357249030935453 50-54 23.591195557883196 27.23524188531144 26.044150977168677 23.12941157963668 55-59 23.247213062736165 26.53855844444644 27.44812906309554 22.766099429721855 60-64 22.412356204290056 27.37765118106926 27.619192507221115 22.590800107419568 65-69 23.100268925162062 27.623620326254965 26.613863336569533 22.662247412013443 70-74 22.719226398727198 27.367299997559634 26.90575289393482 23.007720709778347 75-79 23.187168578506505 26.466111039588753 27.119528521630304 23.227191860274434 80-84 23.954653139583097 26.070065219318412 26.976043927357974 22.999237713740524 85-89 23.08241407421124 27.936788945993897 26.52771012085277 22.453086858942097 90-94 23.954461385394627 25.656647970786377 27.552955010591546 22.83593563322745 95-99 23.599640081015867 27.40152325263667 26.062119763926507 22.93671690242095 100-104 24.208100513240964 25.97301064646687 26.72587956759517 23.09300927269699 105-109 24.539734545441657 27.857270641228414 25.900466502672124 21.702528310657797 110-114 25.508399443376184 27.845993765498257 25.724980044773083 20.920626746352475 115-119 25.49370480523893 28.95700556482143 24.565000709867746 20.98428892007189 120-124 26.52373003724274 28.454735383528785 24.290159797333853 20.731374781894623 125-129 27.234362408609403 28.405853996466167 23.947957808177165 20.41182578674727 130-134 27.119053414906922 27.957621481697014 24.137238914232235 20.78608618916383 135-139 28.685060079041858 28.003930722811287 23.95381386290041 19.357195335246445 140-144 28.683265138503184 27.836439780398692 23.90695583425948 19.57333924683865 145-149 28.445220072564315 28.48686057905371 24.090133659629352 18.97778568875262 150-151 28.015964005330012 29.040204241534706 24.102577548606 18.84125420452928 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 242.0 1 265.0 2 253.0 3 193.5 4 164.0 5 169.5 6 181.5 7 201.5 8 222.0 9 240.0 10 290.0 11 375.0 12 473.5 13 619.0 14 924.0 15 1679.0 16 4143.0 17 7121.5 18 9606.0 19 9473.5 20 8326.5 21 9652.5 22 12409.0 23 17162.5 24 25735.0 25 39966.5 26 66777.0 27 88133.0 28 92677.5 29 82878.0 30 77175.5 31 81670.5 32 86674.0 33 108833.5 34 113538.5 35 128203.5 36 234438.5 37 342224.5 38 371891.0 39 398198.5 40 491835.5 41 653711.0 42 803690.5 43 800578.0 44 862119.0 45 1090757.0 46 1442327.0 47 1630661.5 48 1712202.0 49 1777592.5 50 1503577.0 51 1247265.0 52 1083484.5 53 997111.5 54 828453.5 55 485234.5 56 291084.0 57 185275.5 58 99894.0 59 66534.5 60 46905.5 61 48030.0 62 61104.5 63 57010.5 64 36975.5 65 18833.5 66 14135.5 67 11327.5 68 6382.0 69 5458.5 70 4222.5 71 3721.0 72 3459.0 73 3892.0 74 4018.0 75 3312.0 76 2581.5 77 2569.0 78 2626.0 79 1589.0 80 1768.5 81 1809.0 82 1563.0 83 1428.5 84 1167.5 85 1051.5 86 651.5 87 379.0 88 240.0 89 132.5 90 61.5 91 40.0 92 60.5 93 55.0 94 18.0 95 11.0 96 10.0 97 10.0 98 7.5 99 10.5 100 32.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 9.603677824459656E-6 35-39 4.801838912229828E-6 40-44 0.0 45-49 3.130798970773848E-4 50-54 3.6686049289435884E-4 55-59 0.0012196670837063763 60-64 4.744216845283069E-4 65-69 0.0014636005004476514 70-74 0.0029368046787197626 75-79 0.002285675322221398 80-84 0.0055557276214499105 85-89 0.006429662303475739 90-94 0.007108642325665037 95-99 0.00427747810301433 100-104 0.004353347157827561 105-109 0.005597023436095087 110-114 0.005939874734428296 115-119 0.00844067243991759 120-124 0.0076387653415752104 125-129 0.008887243458754964 130-134 0.008576084297242473 135-139 0.008182333506439626 140-144 0.003394900110946488 145-149 0.006726415948251542 150-151 0.006640943215613852 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 2.0825355E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 6.540338158015564 #Duplication Level Percentage of deduplicated Percentage of total 1 81.43554640156698 5.326160115490156 2 8.831539704249943 1.155225122434708 3 2.708471362964608 0.5314295581526954 4 1.5751630406838222 0.41208395760320893 5 0.8947768564113606 0.2926071608448218 6 0.44085155771848106 0.17299909589800694 7 0.5331898097881107 0.2441069160895568 8 0.3564631324083468 0.1865111541452852 9 0.2339982346641639 0.13773848247740803 >10 1.8247937035270692 2.4433283780650537 >50 0.326766469907554 1.4805837490008178 >100 0.4694304930227791 7.221458020740676 >500 0.13059081454719576 6.003154384125674 >1k 0.1769845493136128 25.641943265522027 >5k 0.036713239872110726 16.605100682451692 >10k+ 0.02472062935358915 32.14556995695828 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG 117750 0.5654165319150621 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 84882 0.40758969054789224 Illumina Single End PCR Primer 1 (100% over 50bp) CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC 79344 0.38099710665196346 No Hit CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG 71666 0.3441285874838628 No Hit CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT 67816 0.325641507671778 No Hit CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG 66642 0.32000414878882016 No Hit ATCAAACCGTACTCACTTCACATTACAGCCCGACCGGCCCAGTCCTTAGA 58536 0.28108044256628517 No Hit GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA 57598 0.2765763176666136 No Hit CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA 55607 0.267015856392364 No Hit GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG 54474 0.26157537290480765 No Hit GTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAATTAAAA 51454 0.24707381938987352 No Hit CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG 50440 0.2422047547328725 No Hit GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC 48815 0.23440176650049901 No Hit GTCAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTGACTTTCGTTC 47510 0.22813536672003912 No Hit CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA 46901 0.22521104682249113 No Hit GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT 46507 0.22331912229107256 No Hit CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC 43554 0.20913929198325792 No Hit CTCAGATGGTAGCTTCGCACCATTGGCCGATCGGCCAAGCACATGAACCA 43457 0.20867351360877162 No Hit GAACAATCCAACGCTTGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCG 43398 0.20839020511295006 No Hit CCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTC 42365 0.20342990551661666 No Hit CTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGA 42094 0.20212860717140238 No Hit CTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAAC 40699 0.19543004188884175 No Hit CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC 39883 0.1915117413364622 No Hit CTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTGGATC 38035 0.18263794302666148 No Hit CTGGAATCGGTTCAGCCGGAGATAGGGATGTTGTTTCCGTAAAGCGCCAC 37611 0.18060196332787604 No Hit AAACAGTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAAT 37018 0.17775447285292376 No Hit CTCACTTCACATTACAGCCCGACCGGCCCAGTCCTTAGAGCCAATCCTTA 36731 0.1763763450851138 No Hit CTCCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAG 36212 0.1738841906896665 No Hit CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG 36134 0.1735096472545126 No Hit GTCGCAATGGTTAGCGTTGAAGACTTTGGGCGTAGGCCCGGTTGAAGCTT 35723 0.17153609146158613 No Hit ATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAG 35619 0.17103670021471423 No Hit CTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCT 35030 0.16820841709541087 No Hit CTCCATTCCTCTATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGG 34979 0.16796352331088715 No Hit CAACGGTTGAGTATAAGTCTCCCGCTCAAGCGCCATCCATTTTCAGGGCT 34598 0.1661340226853276 No Hit CAGAAATCAGAGACAGTCAACGCCCGCAAGGGCCATCTGTCTCCTCTGTT 33307 0.15993484864963886 No Hit GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA 33071 0.15880161466635262 No Hit CTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATTC 33060 0.1587487944383181 No Hit GTCGATCCTAAGATATTGGCTAGTGCCGTTTTTTAAAATAAAATAGAGGA 32619 0.15663118347802474 No Hit GTCAATTTCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGAACCGA 31959 0.15346196979595306 No Hit CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC 31928 0.15331311278967394 No Hit CTGACTTTCGTTCTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCA 30929 0.14851607571635633 No Hit CTCGGATTTTCATGGATCGAACAAAGCGCACGAGACACCACAGGAACCGT 30654 0.14719557001549313 No Hit ATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGGATCGACTGACCC 30649 0.147171560820932 No Hit CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT 30606 0.1469650817477061 No Hit CTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAG 30498 0.1464464831451853 No Hit CTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAA 30326 0.14562056685228175 No Hit CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG 30294 0.1454669080070904 No Hit CCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTT 29994 0.14402635633342145 No Hit GGGAGTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCT 29650 0.1423745237476144 No Hit CACGCTTTCGCGGTCTGTATTCGTACTGGAAATCAAGATCAAGCGAGCTT 29613 0.1421968557078619 No Hit GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC 29395 0.1411500548249958 No Hit CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA 29394 0.14114525298608355 No Hit CTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTT 29149 0.13996880245258725 No Hit CTAGAGTCTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAAC 28985 0.13918130087098154 No Hit GTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAATCACCG 28955 0.13903724570361464 No Hit AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG 28522 0.13695804945461915 No Hit GCCAGATCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTA 28286 0.1358248154713329 No Hit CTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTTCATGTGCTTGGC 28221 0.13551269594203796 No Hit GTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCTAAGG 28003 0.13446589505917184 No Hit CTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTGC 27783 0.1334094904984813 No Hit CGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGT 27761 0.13330385004241224 No Hit GTTAATATTCCCGTACCTGGACACGGAGATAAATGCTCATCGTGAGAATT 27699 0.133006136029854 No Hit GTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAA 27287 0.1310277783980153 No Hit CTCACGTTCCCTATTGGTGGGTGAACAATCCAACGCTTGGCGAATTCTGC 27217 0.1306916496741592 No Hit GTTCGATTAGTCTTTCGCCCCTATACCAAAGTCAGACGATCGATTTGCAC 26803 0.12870368836449605 No Hit GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC 26372 0.12663409579332502 No Hit CGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGGTA 26317 0.12636999465315238 No Hit CTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAACCGAGATC 25895 0.12434361863219137 No Hit GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT 25761 0.1237001722179526 No Hit GTTTCCGTAAAGCGCCACGGTTCCTGTGGTGTCTCGTGCGCTTTGTTCGA 25605 0.12295108534764473 No Hit GGAAAACTGGCAATATTAGCCTAGGAGCCTAGTTCTCATCCAATGGATGG 25477 0.12233644996687931 No Hit CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG 25371 0.12182745504218295 No Hit CTCAGAATGAGGAGGTAGTGACGAAAAATAACGAGACCGTTCTCTTTGAG 24713 0.11866784503793573 No Hit GTTTACGGTCAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTGACT 24668 0.11845176228688539 No Hit GTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTG 24657 0.11839894205885086 No Hit TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT 24325 0.11680473153999056 No Hit CTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAATTAAAACAGAG 24118 0.11581075088515898 No Hit CGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCCACCAAAG 23918 0.114850383102713 No Hit GTCAGATAGGTGATTAACGTTCACATCGAGATGTAACTTTATTGCATACA 23869 0.11461509299601376 No Hit CACAGACCTGTTATTGCTCAACTTCATGCGGCTAAACACCGCTTGTCCCT 23863 0.11458628196254037 No Hit CTCACGTTGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTGCCC 23370 0.11221897537881106 No Hit CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT 23344 0.11209412756709311 No Hit CGGAGATAGGGATGTTGTTTCCGTAAAGCGCCACGGTTCCTGTGGTGTCT 23173 0.11127301311310178 No Hit GGCGAATTCTGCTTCGCAATGATAGGAAGAGCCGACATCGAAGGATCAAA 22974 0.11031744716956805 No Hit CTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCAGTCGTACGTCTC 22657 0.1087952642343912 No Hit GGAATAATGAAATAGGATCTCGGTTCTATTTTGTTGGTTTTCTGATCTGA 22652 0.10877125503983005 No Hit ATTTTTTCAAGGTAAACTTGCTAGCCACCATATGCAACCAATTAAGGTAA 22590 0.1084735410272718 No Hit CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCC 22493 0.1080077626527855 No Hit GTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGGCAACCA 22272 0.10694655625318272 No Hit GTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATTTTCATGGATC 22053 0.10589495353140439 No Hit CTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGAG 21956 0.1054291751569181 No Hit CAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGG 21737 0.10437757243513977 No Hit CTTGGACCGTAGCGAGACGTACGACTGCGAAAGCATTTGCCAAGAATGTC 21717 0.10428153565689516 No Hit CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT 21561 0.10353244878658731 No Hit CTCCACTTCAGTCTTCAAGGATCTCACTTGAATATTTGCTACTACCACCA 21494 0.10321072557946791 No Hit GAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGC 21398 0.10274974904389385 No Hit CTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCTCATAGATCCTC 21267 0.10212070814639174 No Hit GGGCATTCGTATCGCTGCGTGAGAGGTGAAATTCTTGGACCGTAGCGAGA 21233 0.10195744562337591 No Hit CTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGGAAG 21223 0.10190942723425363 No Hit CTCCGTGTCCAGGTACGGGAATATTAACCCGTTTCCCTTTCGATTTATCA 20857 0.10015195419237752 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0010900174330761708 0.0 0.0 2 0.0011380358221984692 0.0 0.0 3 0.001253279956091985 0.0 0.0 4 0.001642228907982601 0.0 0.0 5 0.0017334638473149677 0.0 0.0 6 0.0018198969477351047 0.0 0.0 7 0.0019591502761897696 0.0 0.0 8 0.002175233027240112 0.0 0.0 9 0.004004733652799676 0.0 0.0 10-11 0.005214797058681593 0.0 0.0 12-13 0.005697381869360691 0.0 0.0 14-15 0.007193154690520282 0.0 0.0 16-17 0.008549674183225208 0.0 0.0 18-19 0.009334774845374785 0.0 0.0 20-21 0.010230317802505648 0.0 0.0 22-23 0.011025022142479683 0.0 0.0 24-25 0.01224468922618606 0.0 0.0 26-27 0.014878497869544122 0.0 0.0 28-29 0.017435477090306503 0.0 0.0 30-31 0.025591400482728864 0.0 0.0 32-33 0.029807415047666652 0.0 0.0 34-35 0.03453242453730081 0.0 0.0 36-37 0.04003053009180396 0.0 0.0 38-39 0.045965602987320026 0.0 0.0 40-41 0.05300029699373672 0.0 0.0 42-43 0.0615451693380497 0.0 0.0 44-45 0.07147297128908486 0.0 0.0 46-47 0.08293736169203358 0.0 0.0 48-49 0.09652176397473176 0.0 0.0 50-51 0.11244466180768586 0.0 0.0 52-53 0.13277084592315475 0.0 0.0 54-55 0.15631426210981758 0.0 0.0 56-57 0.18302209013963988 0.0 0.0 58-59 0.21141536362765484 0.0 0.0 60-61 0.24675449710221026 0.0 2.4009194561149136E-6 62-63 0.28796627956642273 0.0 4.801838912229827E-6 64-65 0.33572536938746067 0.0 4.801838912229827E-6 66-67 0.3915995669701669 0.0 4.801838912229827E-6 68-69 0.45897656966711975 0.0 4.801838912229827E-6 70-71 0.5417314614804886 0.0 4.801838912229827E-6 72-73 0.6474799589250699 0.0 4.801838912229827E-6 74-75 0.7781331938879313 0.0 4.801838912229827E-6 76-77 0.9230070747893613 0.0 4.801838912229827E-6 78-79 1.0871843481179553 0.0 4.801838912229827E-6 80-81 1.2973200216755008 0.0 4.801838912229827E-6 82-83 1.5436903716647326 0.0 4.801838912229827E-6 84-85 1.8127302031586017 0.0 4.801838912229827E-6 86-87 2.139800257906768 0.0 4.801838912229827E-6 88-89 2.5146221997176035 0.0 4.801838912229827E-6 90-91 2.9436617046864266 0.0 4.801838912229827E-6 92-93 3.4323952700926346 2.4009194561149136E-6 4.801838912229827E-6 94-95 4.015307302084406 4.801838912229827E-6 4.801838912229827E-6 96-97 4.647899159462107 4.801838912229827E-6 4.801838912229827E-6 98-99 5.384069083096062 4.801838912229827E-6 4.801838912229827E-6 100-101 6.155546928251644 9.603677824459654E-6 4.801838912229827E-6 102-103 6.9697875498400865 9.603677824459654E-6 4.801838912229827E-6 104-105 7.916729390687458 9.603677824459654E-6 4.801838912229827E-6 106-107 8.9532591401203 9.603677824459654E-6 4.801838912229827E-6 108-109 10.015370686358047 9.603677824459654E-6 4.801838912229827E-6 110-111 11.139361129738244 9.603677824459654E-6 4.801838912229827E-6 112-113 12.514602992361954 9.603677824459654E-6 4.801838912229827E-6 114-115 13.877739419087934 9.603677824459654E-6 4.801838912229827E-6 116-117 15.353099623031635 9.603677824459654E-6 4.801838912229827E-6 118-119 16.927872297975235 9.603677824459654E-6 4.801838912229827E-6 120-121 18.425380983901597 9.603677824459654E-6 4.801838912229827E-6 122-123 20.059043411264778 9.603677824459654E-6 4.801838912229827E-6 124-125 21.788977906979255 1.4405516736689482E-5 4.801838912229827E-6 126-127 23.401577067953944 1.4405516736689482E-5 4.801838912229827E-6 128-129 25.111809618611545 1.4405516736689482E-5 4.801838912229827E-6 130-131 26.772503998131125 1.4405516736689482E-5 4.801838912229827E-6 132-133 28.39537669345853 1.4405516736689482E-5 4.801838912229827E-6 134-135 29.94355918542565 1.4405516736689482E-5 4.801838912229827E-6 136-137 31.488495634288107 1.4405516736689482E-5 4.801838912229827E-6 138-139 32.9353089058986 1.4405516736689482E-5 4.801838912229827E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAATC 5070 0.0 100.80499 2 CCAGACG 5175 0.0 100.160515 6 CTTGTTA 12590 0.0 95.58357 1 CAGACGT 5520 0.0 93.76916 7 GTCCAAT 5580 0.0 93.02072 1 GTTACGA 14340 0.0 83.059494 4 TTACGAC 14620 0.0 81.46875 5 ATCAAAG 7020 0.0 76.21143 6 CCTGGCC 13905 0.0 72.72841 5 CAAAGGA 7385 0.0 69.696144 8 TCAAAGG 8195 0.0 69.61882 7 CAACCAG 7580 0.0 67.13806 3 ACCTGGC 15040 0.0 67.09533 4 CCGGCAT 1140 0.0 61.04741 1 CAAACCG 14755 0.0 60.6285 3 CGCCCTT 1880 0.0 60.540035 1 TCAAACC 14925 0.0 60.229355 2 TGGCTCA 4405 0.0 59.57493 3 CACCTGG 17120 0.0 59.028248 3 CTAGCAT 3875 0.0 58.182125 1 >>END_MODULE