FastQCFastQC Report
Wed 5 Aug 2015
H55MKBCXX l01n02 7_m30a.3420000000ed55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55MKBCXX l01n02 7_m30a.3420000000ed55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14345749
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1071350.7468065975502569Illumina Single End PCR Primer 1 (100% over 50bp)
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG781990.5451022459684747No Hit
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG579000.4036038829342407No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC568870.3965425576594153No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG504530.351693034640436No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA472840.3296028670235343No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG409410.285387678259253No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA379790.2647404468041369No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT366160.25523937439585764No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC364740.25424953413028484No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG350980.24465784254276302No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT348160.2426921034238087No Hit
ATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAG319860.22296500517330953No Hit
ATCAAACCGTACTCACTTCACATTACAGCCCGACCGGCCCAGTCCTTAGA309820.21596641625334445No Hit
GTCAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTGACTTTCGTTC307850.21459318715251466No Hit
CTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTGGATC306080.21335937217359652No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAATCACCG304840.21249500461774426No Hit
CTCAGATGGTAGCTTCGCACCATTGGCCGATCGGCCAAGCACATGAACCA297790.20758065682035842No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA293570.2046390188480225No Hit
GAACAATCCAACGCTTGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCG291740.20336337963253087No Hit
GTCGATCCTAAGATATTGGCTAGTGCCGTTTTTTAAAATAAAATAGAGGA288390.20102819309051065No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC285400.19894395196793138No Hit
CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG275360.19194536304796633No Hit
CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC261310.18215152098367257No Hit
CAGAAATCAGAGACAGTCAACGCCCGCAAGGGCCATCTGTCTCCTCTGTT259460.18086194035598976No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA258270.18003242633061542No Hit
CTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAAC245180.17090777205149763No Hit
CTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGA241130.16808463608278662No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC239530.16696932310749338No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT232570.1621177116649678No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC232280.1619155611881959No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT223000.1554467459314951No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA217280.15145950204482178No Hit
GTCAATTTCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGAACCGA216580.150971552618131No Hit
GGGAGTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCT215850.15046269107315344No Hit
CGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGT214340.14941011445272045No Hit
CCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTT212680.1482529772408537No Hit
CACGCTTTCGCGGTCTGTATTCGTACTGGAAATCAAGATCAAGCGAGCTT209660.14614782399998774No Hit
GTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAATTAAAA209490.14602932199636284No Hit
GTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCTAAGG208060.1450325110246945No Hit
CTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCT203680.14197934175482926No Hit
CTGGAATCGGTTCAGCCGGAGATAGGGATGTTGTTTCCGTAAAGCGCCAC203540.1418817518694911No Hit
CAACGGTTGAGTATAAGTCTCCCGCTCAAGCGCCATCCATTTTCAGGGCT203520.14186781045729993No Hit
GTCGCAATGGTTAGCGTTGAAGACTTTGGGCGTAGGCCCGGTTGAAGCTT203050.14154018727080755No Hit
CTCACTTCACATTACAGCCCGACCGGCCCAGTCCTTAGAGCCAATCCTTA202280.14100344290144767No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG201550.14049458135647014No Hit
CTAGAGTCTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAAC201530.14048063994427895No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG200270.13960233097623553No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT199930.1393653269689857No Hit
CTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATTC199060.13875887553867003No Hit
CGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGGTA198050.13805483422301618No Hit
CACAGACCTGTTATTGCTCAACTTCATGCGGCTAAACACCGCTTGTCCCT197890.13794330292548684No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT195310.1361448607528265No Hit
CTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAACCGAGATC192290.1340397075119605No Hit
CTGACTTTCGTTCTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCA191650.1335935823218432No Hit
CTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAA189650.13219944110272666No Hit
GCCAGATCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTA188550.13143266343221258No Hit
CTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTTCATGTGCTTGGC185310.12917415465724377No Hit
CTTGACTCTAGTCCGACTCTGTGAAGAGTCATGAGAGGTGTAGCATAAGT184830.1288395607646558No Hit
GTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTAC183850.12815643156728868No Hit
CCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTC182430.12716659130171593No Hit
GTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAA181450.12648346210434883No Hit
CTCACGTTCCCTATTGGTGGGTGAACAATCCAACGCTTGGCGAATTCTGC179850.12536814912905558No Hit
GTTAATATTCCCGTACCTGGACACGGAGATAAATGCTCATCGTGAGAATT179600.12519388147666602No Hit
CTCGGATTTTCATGGATCGAACAAAGCGCACGAGACACCACAGGAACCGT177820.12395309579165227No Hit
CTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTT174180.12141575877286016No Hit
GTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGGCAACCA173080.12064898110234606No Hit
GTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTGGAT170870.11910845505522227No Hit
CGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCCACCAAAG170550.11888539246016362No Hit
GTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTG170140.11859959351024474No Hit
CTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTGC167630.11684994628025347No Hit
CTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAG163640.11406863454811597No Hit
ATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGGATCGACTGACCC163290.11382465983477057No Hit
GTGAAATACCACTACTTTCATCGATTCTTTACTTATTCGGTAAAACGAGA161650.11268146403509499No Hit
CTTTCATCGATTCTTTACTTATTCGGTAAAACGAGAATTGAAATATTATA160060.11157312176589733No Hit
CTTGCTAGAAACCTTCTAAAAAATTCAAAAGGATCGATAGGCCACGCTTT159940.11148947329275033No Hit
GTCAGATAGGTGATTAACGTTCACATCGAGATGTAACTTTATTGCATACA159640.11128035210988287No Hit
CGAGACTCTAGCCTACTAAATAGTTACTGGATAATTTTATCGTCCAGACA159120.11091787539291256No Hit
GGCGAATTCTGCTTCGCAATGATAGGAAGAGCCGACATCGAAGGATCAAA158460.11045780879060411No Hit
ATTTTTTCAAGGTAAACTTGCTAGCCACCATATGCAACCAATTAAGGTAA157930.11008836136753822No Hit
GTTTACGGTCAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTGACT156190.10887545850690682No Hit
CGGAGATAGGGATGTTGTTTCCGTAAAGCGCCACGGTTCCTGTGGTGTCT155860.10864542520575259No Hit
GAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGC151180.10538313475301987No Hit
GTTGAGCTTGACTCTAGTCCGACTCTGTGAAGAGTCATGAGAGGTGTAGC150880.10517401357015238No Hit
GCCAGTTCTAAGTTGACTGTTTAACGCCGGCCGAAATATCAAATAAAACA149670.10433055813258688No Hit
CTTTAACTCAACGTTTGGTTCATCCCACAGCGCCAGTTCTGCTTACCAAA149500.10421205612896198No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT148790.10371713599617559No Hit
GTTGAAGCTTCCATTGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAG148460.10348710269502137No Hit
GTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATTTTCATGGATC148330.10339648351577878No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG147830.10304794821099963No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC146780.10231602407096346No Hit
GTTTTATTTGATATTTCGGCCGGCGTTAAACAGTCAACTTAGAACTGGCA145830.10165380699188309No Hit
CTCCGTGTCCAGGTACGGGAATATTAACCCGTTTCCCTTTCGATTTATCA144460.10069882025678827No Hit
GGCATTCGTATCGCTGCGTGAGAGGTGAAATTCTTGGACCGTAGCGAGAC144390.10065002531411918No Hit
AAACAGTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAAT144150.1004827283678252No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAATC43250.0106.603452
GTCCAAT47050.099.688481
CCAGACG32000.098.093046
CTTGTTA93250.096.088171
CAGACGT33600.093.63777
GTTACGA103600.086.2087254
TTACGAC106700.083.636135
ATCAAAG63400.076.838756
TCAAAGG68700.073.548927
CAAAGGA67650.068.7966848
CCTGGCC81150.063.158415
TGGCTCA27300.061.8719143
ACCTGGC84750.060.2189564
CAACCAG51700.060.0141263
CTAGCAT32400.057.502721
CCGGCAT9000.056.383991
GATAGGT43700.055.904765
GTTAGTT212150.055.015251
CCAATCA87100.054.183013
CAAACCG91300.053.3579033
Produced by FastQC (version 0.11.2)