FastQCFastQC Report
Wed 5 Aug 2015
H55MKBCXX l01n02 6_f30b.3420000000ed48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55MKBCXX l01n02 6_f30b.3420000000ed48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25186387
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3170501.2588149304622374Illumina Single End PCR Primer 1 (100% over 50bp)
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG1035770.4112419935419876No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA846790.33620939756067436No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC764480.30352904527354396No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA721660.28652779773454606No Hit
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG681430.2705548834773324No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT643240.25539193056947784No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC621670.24682778041963702No Hit
GTCAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTGACTTTCGTTC588610.23370164208149427No Hit
ATCAAACCGTACTCACTTCACATTACAGCCCGACCGGCCCAGTCCTTAGA585350.2324072920820283No Hit
GAACAATCCAACGCTTGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCG580520.2304895894754575No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG577280.22920318027353426No Hit
GCCAGATCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTA572880.22745620481413234No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG546380.21693464807000704No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG542470.21538222215040215No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT536160.21287690052566888No Hit
CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC528220.20972440390120264No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA517810.2055912187802085No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA506030.200914089027537No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC492200.19542302752673496No Hit
GTCGATCCTAAGATATTGGCTAGTGCCGTTTTTTAAAATAAAATAGAGGA486060.192985202681115No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC473830.1881294049837319No Hit
GGGAGTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCT463540.18404386464799416No Hit
CGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGT449030.17828281603073914No Hit
GTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAATTAAAA446400.17723860115386936No Hit
GTCGCAATGGTTAGCGTTGAAGACTTTGGGCGTAGGCCCGGTTGAAGCTT439910.1746618123512515No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT431430.1712949141931314No Hit
CAGAAATCAGAGACAGTCAACGCCCGCAAGGGCCATCTGTCTCCTCTGTT429620.17057627201551379No Hit
CTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAAC425780.16905163888730845No Hit
ATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAG413020.16398541005504283No Hit
CTCAGATGGTAGCTTCGCACCATTGGCCGATCGGCCAAGCACATGAACCA410490.1629808991658867No Hit
CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG394460.15661634993538376No Hit
GTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCTAAGG392500.15583815177619562No Hit
CAACGGTTGAGTATAAGTCTCCCGCTCAAGCGCCATCCATTTTCAGGGCT386770.15356311328020172No Hit
CTCACTTCACATTACAGCCCGACCGGCCCAGTCCTTAGAGCCAATCCTTA386230.15334871174654785No Hit
CGGAGATAGGGATGTTGTTTCCGTAAAGCGCCACGGTTCCTGTGGTGTCT382550.1518876049986844No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC380140.1509307388947847No Hit
GAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGC375480.1490805330673272No Hit
CACGCTTTCGCGGTCTGTATTCGTACTGGAAATCAAGATCAAGCGAGCTT374050.14851276604302158No Hit
CTGGAATCGGTTCAGCCGGAGATAGGGATGTTGTTTCCGTAAAGCGCCAC372570.1479251470248591No Hit
GGGCATTCGTATCGCTGCGTGAGAGGTGAAATTCTTGGACCGTAGCGAGA357690.14201719365306345No Hit
CCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTC351900.13971833276444134No Hit
AAACAGTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAAT344830.13691126083308416No Hit
GCCGGAGATAGGGATGTTGTTTCCGTAAAGCGCCACGGTTCCTGTGGTGT338870.1345449031653488No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT336010.13340936911673754No Hit
GGCGAATTCTGCTTCGCAATGATAGGAAGAGCCGACATCGAAGGATCAAA334630.13286145408628874No Hit
CTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTT332130.13186885439344675No Hit
GTTTCCGTAAAGCGCCACGGTTCCTGTGGTGTCTCGTGCGCTTTGTTCGA330100.13106286344285903No Hit
CTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTGGATC324910.129002226480519No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC324550.12885929212474975No Hit
GATAAATCGAAAGGGAAACGGGTTAATATTCCCGTACCTGGACACGGAGA321360.12759273491668338No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG318680.12652866804595675No Hit
GTTTACGGTCAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTGACT315880.1254169563899737No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAATCACCG309860.12302677632961012No Hit
GTTCGATTAGTCTTTCGCCCCTATACCAAAGTCAGACGATCGATTTGCAC309770.1229910427406678No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA300270.11921916390786817No Hit
GGCATTCGTATCGCTGCGTGAGAGGTGAAATTCTTGGACCGTAGCGAGAC299200.1187943312393318No Hit
GTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTGGAT299050.11873477525776127No Hit
CTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTGC298430.11848861053393644No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT297980.11830994258922489No Hit
GTCAATTTCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGAACCGA294550.11694809581064565No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG294000.11672972387822042No Hit
CGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCCACCAAAG292560.11615798645514341No Hit
CTAGAGTCTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAAC291460.11572124259029291No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG289380.11489539964584837No Hit
CTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGA286250.11365266483041017No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT284010.11276329550562374No Hit
CTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCT283580.11259256835845491No Hit
GTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTG279940.11114734320567694No Hit
GCTGATTCCTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCAT279830.1111036688191919No Hit
GTGTAATCCATCTGATAAATCGAAAGGGAAACGGGTTAATATTCCCGTAC277320.11010709872757853No Hit
GTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATTTTCATGGATC276290.1096981476541276No Hit
CTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAA273260.1084951168264031No Hit
GTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGGCAACCA273200.10847129443377489No Hit
CCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTT272850.108332330476777No Hit
GTTCCCTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATC272530.10820527771609321No Hit
GTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTAC272060.10801866897383891No Hit
CGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGGTA271950.10797499458735388No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGC268550.10662505900508874No Hit
CACAGACCTGTTATTGCTCAACTTCATGCGGCTAAACACCGCTTGTCCCT267150.10606920317709721No Hit
GTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAA266230.10570392649013136No Hit
GCGGATATGGGTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATT265980.10560466652084716No Hit
GCCGCAACGAGTAAGAGGGTCGCAATGGTTAGCGTTGAAGACTTTGGGCG265240.10531085701176592No Hit
CTGACTTTCGTTCTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCA262390.10417929336192602No Hit
CTCGGATTTTCATGGATCGAACAAAGCGCACGAGACACCACAGGAACCGT261790.10394106943564395No Hit
GACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTG260490.10342491759536611No Hit
CTCACGTTCCCTATTGGTGGGTGAACAATCCAACGCTTGGCGAATTCTGC259680.10310331529488528No Hit
GGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTA254240.10094341836326107No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAATC77000.0109.8687742
GTCCAAT81900.0103.915021
CTTGTTA130300.089.628331
ATCAAAG106900.083.478486
CCAGACG42400.082.0671846
CAAAGGA109000.079.27648
TCAAAGG115050.077.690977
CAGACGT44900.077.497747
GTTACGA154050.074.916164
TTACGAC157250.073.391645
GCACGAC30050.070.200898
CCAATCA123050.070.165613
CCGGCAT15300.069.649831
GCGAGCG32250.064.288081
CAATCAA141200.061.711194
AAGCACG34850.061.1559526
ACCTGGC131250.059.872054
CCTGGCC132700.059.381725
CTCCGAT30300.058.6161961
CGCGGAT14700.056.218811
Produced by FastQC (version 0.11.2)