##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H55MKBCXX l01n02 3_ym.3420000000ed11.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 29708294 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.9980327042677 35.0 35.0 35.0 32.0 35.0 2 33.98607355238911 35.0 35.0 35.0 32.0 35.0 3 33.844691418497476 35.0 35.0 35.0 31.0 35.0 4 33.97214161809493 35.0 35.0 35.0 32.0 35.0 5 33.941680898943574 35.0 35.0 35.0 32.0 35.0 6 38.370286627700665 40.0 39.0 40.0 36.0 40.0 7 38.5122734748754 40.0 39.0 40.0 36.0 40.0 8 38.46710982461666 40.0 39.0 40.0 36.0 40.0 9 38.539204304360254 40.0 39.0 40.0 36.0 40.0 10-14 38.56361420820731 40.0 39.0 40.0 36.0 40.0 15-19 38.55246651322354 40.0 39.0 40.0 36.0 40.0 20-24 38.538920222076705 40.0 39.0 40.0 36.0 40.0 25-29 38.52981559291153 40.0 39.0 40.0 36.0 40.0 30-34 38.512071544734276 40.0 39.0 40.0 36.0 40.0 35-39 38.48310611844625 40.0 39.0 40.0 36.0 40.0 40-44 38.394952345631154 40.0 39.0 40.0 36.0 40.0 45-49 38.309414475297714 40.0 39.0 40.0 35.4 40.0 50-54 38.269860329240046 40.0 39.0 40.0 35.0 40.0 55-59 38.2692500619524 40.0 39.0 40.0 35.0 40.0 60-64 38.26601413059935 40.0 39.0 40.0 35.0 40.0 65-69 38.21255969797525 40.0 39.0 40.0 35.0 40.0 70-74 38.12622456207011 40.0 39.0 40.0 34.8 40.0 75-79 38.08148575613261 40.0 39.0 40.0 34.0 40.0 80-84 37.98729325218069 40.0 39.0 40.0 34.0 40.0 85-89 38.02980085628612 40.0 39.0 40.0 34.0 40.0 90-94 38.005983096841575 40.0 39.0 40.0 34.0 40.0 95-99 37.95148094333522 40.0 39.0 40.0 34.0 40.0 100-104 36.93027942971077 39.0 37.6 39.4 32.0 39.6 105-109 37.93027575396958 40.0 39.0 40.0 34.0 40.0 110-114 37.96890552517085 40.0 39.0 40.0 34.0 40.0 115-119 37.891626755814386 40.0 39.0 40.0 34.0 40.0 120-124 37.82869367726064 40.0 39.0 40.0 34.0 40.0 125-129 37.76813409750153 40.0 39.0 40.0 34.0 40.0 130-134 37.65259191928019 40.0 39.0 40.0 34.0 40.0 135-139 37.57677863966204 40.0 39.0 40.0 34.0 40.0 140-144 37.469763750150044 40.0 39.0 40.0 33.0 40.0 145-149 37.32255087417676 40.0 39.0 40.0 31.0 40.0 150-151 35.4933750655625 38.5 35.0 39.5 23.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.3579531349562046 2201 2 0.39471759343410895 2201 3 0.3995906421887554 2201 4 0.3549639725201388 2201 5 0.3537589184669585 2201 6 0.595011107582863 2201 7 0.5331992549360081 2201 8 0.5960243180499347 2201 9 0.5744483716581996 2201 10-14 0.542095916954743 2201 15-19 0.5358556043013891 2201 20-24 0.5126602312119033 2201 25-29 0.5247233194314731 2201 30-34 0.5219537000140235 2201 35-39 0.5226612951932879 2201 40-44 0.536861248578127 2201 45-49 0.5641472890379475 2201 50-54 0.5725404905550278 2201 55-59 0.591115179403829 2201 60-64 0.567783399483524 2201 65-69 0.5502574528118274 2201 70-74 0.5704295892345499 2201 75-79 0.5510439998450636 2201 80-84 0.6210662939591387 2201 85-89 0.5622016460813128 2201 90-94 0.5809484663264755 2201 95-99 0.5600267669268391 2201 100-104 0.6208485240493573 2201 105-109 0.5836115052849067 2201 110-114 0.5460895171526445 2201 115-119 0.5554725562274783 2201 120-124 0.5860109662773496 2201 125-129 0.6019677563670669 2201 130-134 0.5986166941976734 2201 135-139 0.5964323110827863 2201 140-144 0.5953095627297245 2201 145-149 0.5961515737510084 2201 150-151 0.684722603562399 1101 1 0.3592550107856525 1101 2 0.3261837131594447 1101 3 0.3468994697015759 1101 4 0.2648584210066147 1101 5 0.34304058569153995 1101 6 0.5009660550539721 1101 7 0.5039037904274082 1101 8 0.5834745306544349 1101 9 0.49610500224729037 1101 10-14 0.5179629927114746 1101 15-19 0.4905296928725704 1101 20-24 0.49278998840118504 1101 25-29 0.47473200800043713 1101 30-34 0.5097779112896106 1101 35-39 0.4964550100801901 1101 40-44 0.520183221100524 1101 45-49 0.5295936415203073 1101 50-54 0.5384747513641628 1101 55-59 0.5197846268372928 1101 60-64 0.500982617433742 1101 65-69 0.5164921333957508 1101 70-74 0.5039897719406241 1101 75-79 0.5128472209161998 1101 80-84 0.5712323782963225 1101 85-89 0.5212471314990665 1101 90-94 0.4852051810703486 1101 95-99 0.5220201461374714 1101 100-104 0.6309329841492826 1101 105-109 0.5076622605432561 1101 110-114 0.4879949862123638 1101 115-119 0.5270414931100902 1101 120-124 0.5121999273416264 1101 125-129 0.5327106143086837 1101 130-134 0.5285055193229695 1101 135-139 0.5497076865751112 1101 140-144 0.5359534779596586 1101 145-149 0.5489431943455969 1101 150-151 0.5572597856578483 2202 1 0.2878222484277373 2202 2 0.24699450978859971 2202 3 0.3038790086800205 2202 4 0.2724285903954282 2202 5 0.3064555118206158 2202 6 0.4503392120809764 2202 7 0.4453729755452187 2202 8 0.499096206990167 2202 9 0.47499035303933823 2202 10-14 0.43922155770886206 2202 15-19 0.4187525660491289 2202 20-24 0.4221652322908369 2202 25-29 0.4523898351209681 2202 30-34 0.44039597760746574 2202 35-39 0.42688481010240054 2202 40-44 0.4446697697888311 2202 45-49 0.44427421854983606 2202 50-54 0.4657410045966941 2202 55-59 0.4263754948311771 2202 60-64 0.4166108449113324 2202 65-69 0.38770488401085146 2202 70-74 0.384998254172757 2202 75-79 0.42841958868052643 2202 80-84 0.48291179279529217 2202 85-89 0.4162401329742522 2202 90-94 0.424808901908591 2202 95-99 0.4426930467588335 2202 100-104 0.3624584824437207 2202 105-109 0.4207245302139171 2202 110-114 0.35733213842679135 2202 115-119 0.38236638528817224 2202 120-124 0.3935768506558759 2202 125-129 0.4136336448073976 2202 130-134 0.4356197559197952 2202 135-139 0.4459954798669301 2202 140-144 0.4373863801353295 2202 145-149 0.4896246679381946 2202 150-151 0.5849738048113835 1102 1 0.21077625976462144 1102 2 0.2365503760997072 1102 3 0.21180455653536256 1102 4 0.24471690461655982 1102 5 0.27723603248264794 1102 6 0.3432542011970696 1102 7 0.34952692551921416 1102 8 0.45923834036252487 1102 9 0.4368466208055679 1102 10-14 0.40033288867243044 1102 15-19 0.3871234832611279 1102 20-24 0.41341537552347063 1102 25-29 0.4058139170951023 1102 30-34 0.3800571014930938 1102 35-39 0.3813390957377223 1102 40-44 0.4046994846330989 1102 45-49 0.38234578148886555 1102 50-54 0.381809017250788 1102 55-59 0.35385721020860217 1102 60-64 0.39411111216767125 1102 65-69 0.36801511543835375 1102 70-74 0.35958628514356406 1102 75-79 0.3710442341936968 1102 80-84 0.44240726085904214 1102 85-89 0.3618203476925359 1102 90-94 0.3583718266926752 1102 95-99 0.3692779379566602 1102 100-104 0.38214990588419795 1102 105-109 0.3798858180074234 1102 110-114 0.377704251138816 1102 115-119 0.3817328011484378 1102 120-124 0.3918947126166401 1102 125-129 0.38299183403760395 1102 130-134 0.39189282647916457 1102 135-139 0.4043130524321583 1102 140-144 0.40675004479233223 1102 145-149 0.4089786389884651 1102 150-151 0.6678099390911072 2203 1 0.1862633339649875 2203 2 0.21319923314981537 2203 3 0.20331489285391768 2203 4 0.20738070627406557 2203 5 0.22083571397431 2203 6 0.31541626080243645 2203 7 0.28355653155725236 2203 8 0.3256025985954949 2203 9 0.34787299415639694 2203 10-14 0.34160624039009946 2203 15-19 0.32834893164453405 2203 20-24 0.35239517198782266 2203 25-29 0.33670316788816024 2203 30-34 0.3408384264525992 2203 35-39 0.3405705606238598 2203 40-44 0.3392957657874831 2203 45-49 0.29877165518242776 2203 50-54 0.3185780073918707 2203 55-59 0.3055099677518456 2203 60-64 0.32889302755822314 2203 65-69 0.30745087952375627 2203 70-74 0.31546723004275634 2203 75-79 0.2952196675988219 2203 80-84 0.37672798792127793 2203 85-89 0.2915205390222013 2203 90-94 0.30907592538076045 2203 95-99 0.2941981652973453 2203 100-104 0.3108341568187427 2203 105-109 0.307026906970286 2203 110-114 0.3130126781441902 2203 115-119 0.33317780583325884 2203 120-124 0.2923330016102881 2203 125-129 0.32073087127267286 2203 130-134 0.34091486479607624 2203 135-139 0.29700892786629396 2203 140-144 0.3590132789214664 2203 145-149 0.3761533974344715 2203 150-151 0.6656637898932942 1103 1 0.1156602356623111 1103 2 0.20791296504489765 1103 3 0.10603256680214912 1103 4 0.14894002749508672 1103 5 0.12580642061116265 1103 6 0.09100243499441518 1103 7 0.1498166576629032 1103 8 0.24487798788575077 1103 9 0.24543899992328733 1103 10-14 0.24491259557928657 1103 15-19 0.24524167902795568 1103 20-24 0.2322203328863992 1103 25-29 0.21856313903903413 1103 30-34 0.23763166422341442 1103 35-39 0.19110483471698103 1103 40-44 0.22898713424585537 1103 45-49 0.25739963844148406 1103 50-54 0.1776806023378299 1103 55-59 0.19570353882207314 1103 60-64 0.19090204050217352 1103 65-69 0.22703207582718932 1103 70-74 0.22481612758821967 1103 75-79 0.20759173751334004 1103 80-84 0.28637697000299056 1103 85-89 0.23965362588421613 1103 90-94 0.24746697287486086 1103 95-99 0.21196010987858926 1103 100-104 0.24522077927989727 1103 105-109 0.23214666127212524 1103 110-114 0.21905558633343958 1103 115-119 0.23969272411307685 1103 120-124 0.17477307044514845 1103 125-129 0.2691160007952007 1103 130-134 0.27555993188931893 1103 135-139 0.280531197829184 1103 140-144 0.2969927873340552 1103 145-149 0.2957022333605295 1103 150-151 0.49422588174260085 2204 1 -0.05434563376185508 2204 2 -0.0455477908416313 2204 3 -0.008346411596001246 2204 4 0.04570932501007974 2204 5 0.06382693092066205 2204 6 -0.01777972631009561 2204 7 0.031649910922553204 2204 8 0.13641912992467553 2204 9 0.06205934971433891 2204 10-14 0.012284163019046446 2204 15-19 0.06952404370378673 2204 20-24 0.06303534791477006 2204 25-29 0.018456399369306098 2204 30-34 -0.019456259198022963 2204 35-39 0.049134501432035904 2204 40-44 0.038602123608015404 2204 45-49 0.011198515052512903 2204 50-54 0.1234447570089543 2204 55-59 0.1573350644918392 2204 60-64 0.08426584212181609 2204 65-69 0.0829869120131832 2204 70-74 0.04471276228766641 2204 75-79 0.09971239786165143 2204 80-84 0.12733519802353754 2204 85-89 0.09639397252557558 2204 90-94 0.043226842745447414 2204 95-99 0.06464510651206012 2204 100-104 0.17784914043694755 2204 105-109 0.08668582881378484 2204 110-114 0.09384955534168427 2204 115-119 0.13032904906373943 2204 120-124 0.19336480030828085 2204 125-129 0.17306070464804435 2204 130-134 0.1201183488005313 2204 135-139 0.09706366001805833 2204 140-144 0.05370687240592531 2204 145-149 0.19294362205653925 2204 150-151 0.4221987619796792 2205 1 -0.027145317046965545 2205 2 -0.024334534551016418 2205 3 -0.010260810108469798 2205 4 0.08441930326210212 2205 5 0.06531795626662529 2205 6 0.08700485819183257 2205 7 0.06205442202887923 2205 8 0.13579304656821733 2205 9 0.13856912775067798 2205 10-14 0.14989080876066652 2205 15-19 0.15755039175083851 2205 20-24 0.18464774953208263 2205 25-29 0.16462580549880101 2205 30-34 0.12743748056540483 2205 35-39 0.08513295707716395 2205 40-44 0.16138527215339593 2205 45-49 0.17390589970465697 2205 50-54 0.17994011828780998 2205 55-59 0.11065863845064428 2205 60-64 0.16074484775788989 2205 65-69 0.15950365728195237 2205 70-74 0.1504275714257659 2205 75-79 0.1270996299965219 2205 80-84 0.1354019785037366 2205 85-89 0.040652764604118374 2205 90-94 0.04186680379788754 2205 95-99 0.1297863331674165 2205 100-104 0.2272749669968661 2205 105-109 0.0446472711325967 2205 110-114 0.07667439234933937 2205 115-119 0.18627087026774802 2205 120-124 0.0029447213978102127 2205 125-129 0.08609205816547671 2205 130-134 0.14502429077098355 2205 135-139 0.09370042187844518 2205 140-144 0.1345766869924674 2205 145-149 0.032091710194052325 2205 150-151 0.35313165869403207 1216 1 0.01173275275930763 1216 2 0.10125096014767365 1216 3 0.11493186025531088 1216 4 0.06414089525107869 1216 5 0.05851233660357735 1216 6 0.03908347538184387 1216 7 0.08442821605375883 1216 8 0.1921251315023511 1216 9 0.17523108251642583 1216 10-14 0.116673519987323 1216 15-19 0.12377192491176459 1216 20-24 0.18054643715738194 1216 25-29 0.1662354929521399 1216 30-34 0.15634056049688638 1216 35-39 0.15002684908469632 1216 40-44 0.1493980169120377 1216 45-49 0.08413649351730612 1216 50-54 0.1280271736269114 1216 55-59 0.08869283649982407 1216 60-64 0.07536395873022883 1216 65-69 0.10599390030969147 1216 70-74 0.12350440906712379 1216 75-79 0.12999427093043892 1216 80-84 0.18459547019766376 1216 85-89 0.13867132598667098 1216 90-94 0.14886856722539932 1216 95-99 0.1583379157552116 1216 100-104 -0.01203529951684601 1216 105-109 0.15733530878706858 1216 110-114 0.1101841948567781 1216 115-119 0.13317330931282356 1216 120-124 0.07986199388030712 1216 125-129 0.13653912281524327 1216 130-134 0.11081371348676328 1216 135-139 0.16386874647808014 1216 140-144 0.19094042764219665 1216 145-149 0.20951244476412967 1216 150-151 0.37865547514710585 2206 1 -0.0672809222916797 2206 2 -0.023735946161870913 2206 3 -0.0067577687437108125 2206 4 -0.029774949526199634 2206 5 -0.044260083754963375 2206 6 -0.09779298476836829 2206 7 -0.14877793292705377 2206 8 -0.09372250093238677 2206 9 -0.09313391746043465 2206 10-14 -0.09617987648814363 2206 15-19 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 2.0 13 7.0 14 11.0 15 22.0 16 284.0 17 2721.0 18 8536.0 19 20065.0 20 37291.0 21 57879.0 22 82570.0 23 108667.0 24 137640.0 25 168711.0 26 223477.0 27 304786.0 28 262859.0 29 280590.0 30 321138.0 31 372796.0 32 438341.0 33 527970.0 34 647440.0 35 805831.0 36 1060270.0 37 1558964.0 38 3008918.0 39 1.9270505E7 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.06377754306592 13.078802168848874 7.8538269481243175 42.00359333996089 2 20.020644739815758 19.1221145179188 45.063698373255626 15.793542369009813 3 22.340491850524973 22.66431387813787 25.035436232050213 29.959758039286942 4 25.778582237000887 36.03286341517961 18.42878288467187 19.759771463147633 5 25.739047822806654 32.93257768352501 22.068527395076945 19.25984709859139 6 18.592656313418736 35.44283963259553 28.126044531537218 17.838459522448513 7 15.21299742085493 16.845992570290303 45.08058254708264 22.860427461772122 8 18.511584677329505 18.684458959508078 31.722854903751795 31.081101459410625 9 25.875275773156144 20.095691122485864 30.71599803071829 23.313035073639703 10-14 23.473151302461194 27.753065861001648 26.045100401928163 22.728682434609002 15-19 23.510289752753895 26.074018925489295 27.610372376145193 22.805318945611617 20-24 23.835300673946474 26.838331410076933 26.123353969770193 23.2030139462064 25-29 24.197611616473164 26.609892846758555 26.060677196745125 23.131818340023163 30-34 22.88260462769115 26.545561084427206 26.88349165276819 23.688342635113454 35-39 22.29667072275088 27.26241634667127 26.71908251554201 23.721830415035846 40-44 23.36164439465962 26.919429974673065 27.62487539674947 22.09405023391784 45-49 23.123444444694282 26.257983603531 27.10992276435774 23.508649187416975 50-54 23.690441222551186 26.913100674925943 26.35432860510213 23.042129497420742 55-59 23.088706766283554 26.52820450608033 27.57171851788619 22.811370209749928 60-64 22.200895569511154 27.737460418867983 27.523185938149098 22.538458073471766 65-69 22.85461904383618 27.850350816012455 26.572271060742132 22.722759079409236 70-74 22.757012954823733 27.527691666857862 26.868259798575313 22.847035579743093 75-79 23.139184776372925 26.648668733031254 27.068951970275812 23.143194520320005 80-84 23.90252978832882 26.272172164110017 26.97937472683306 22.845923320728097 85-89 22.925684365769158 27.81227928994899 26.640109467068555 22.621926877213294 90-94 23.867813729154523 25.39801383155086 27.70054313012249 23.033629309172134 95-99 23.26716421828309 27.336488953268983 26.35705518144186 23.039291647006063 100-104 23.66509618538626 25.975452347122598 26.89135742302885 23.468094044462294 105-109 24.116425485320224 27.774382163869653 25.9171781322973 22.19201421851282 110-114 25.040971615766754 27.66617658156597 25.713040428193366 21.579811374473906 115-119 24.89253113595961 28.97277897108705 24.674412885623344 21.46027700733 120-124 25.74417303517163 28.440639983387122 24.51394592962607 21.301241051815182 125-129 26.461474297729527 28.331765272808635 24.02175789934124 21.1850025301206 130-134 26.561842257882702 27.78365586842205 24.42265264424265 21.231849229452607 135-139 28.006946006889642 28.11284741833439 24.1081575535078 19.77204902126817 140-144 28.091022707971526 27.823330127318464 24.06225411545339 20.023393049256626 145-149 28.161485544313326 28.225270587888367 24.26753254701177 19.34571132078654 150-151 28.025414819721234 28.3439155479405 24.261908688030985 19.36876094430728 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 341.0 1 321.5 2 263.5 3 221.5 4 220.0 5 224.0 6 250.5 7 303.5 8 371.0 9 433.0 10 500.0 11 636.5 12 746.0 13 915.5 14 1333.5 15 2349.5 16 5561.0 17 8943.0 18 11752.5 19 11638.5 20 10104.5 21 11636.5 22 14556.5 23 18774.5 24 26304.0 25 45043.0 26 76711.5 27 97266.0 28 101223.5 29 95295.0 30 93095.5 31 99154.0 32 113572.5 33 144840.5 34 156994.0 35 193714.5 36 366605.5 37 520367.0 38 553890.0 39 594268.0 40 709286.0 41 975418.5 42 1186520.0 43 1151536.5 44 1235301.0 45 1556125.0 46 2018265.0 47 2263290.5 48 2456029.0 49 2604015.5 50 2208909.0 51 1805384.5 52 1528928.0 53 1336662.5 54 1113995.0 55 689131.0 56 402981.0 57 247780.0 58 137880.0 59 102413.5 60 81967.0 61 93206.0 62 131535.5 63 129654.0 64 87276.0 65 40150.5 66 18462.5 67 11428.5 68 1561.5 69 839.0 70 464.0 71 302.5 72 189.0 73 137.5 74 79.0 75 60.0 76 51.5 77 41.5 78 36.5 79 34.0 80 28.5 81 27.0 82 20.5 83 19.0 84 25.5 85 24.5 86 21.5 87 18.5 88 13.5 89 12.0 90 12.0 91 9.5 92 9.0 93 10.5 94 6.0 95 7.5 96 13.0 97 12.5 98 10.5 99 14.5 100 44.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 6.732126725284191E-6 35-39 7.405339397812611E-6 40-44 0.0 45-49 3.123706800531865E-4 50-54 3.911365627390116E-4 55-59 0.0011646579234741651 60-64 4.5980425533691027E-4 65-69 0.0013935502321338277 70-74 0.0029392465282590784 75-79 0.0022263143080514824 80-84 0.0055290956794759065 85-89 0.006391481112984812 90-94 0.007247134419768433 95-99 0.004214984542700432 100-104 0.004400118027645748 105-109 0.0056246918789749425 110-114 0.005899362649366537 115-119 0.00835052999004251 120-124 0.007356194872718037 125-129 0.008736280851401296 130-134 0.008424583384020638 135-139 0.0079546809385958 140-144 0.003393665082215761 145-149 0.006587386000690582 150-151 0.006481355004767355 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 2.9708294E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 7.298406282982055 #Duplication Level Percentage of deduplicated Percentage of total 1 80.10103800596484 5.846099190561181 2 9.678546233362804 1.4127592527941475 3 3.3950376656641987 0.7433509269013294 4 1.6509790169252456 0.48198062520794993 5 0.994101483099602 0.3627678255087957 6 0.6399021778420727 0.280215964515389 7 0.440752102306827 0.22517515388995865 8 0.33068689529230394 0.19307898514409438 9 0.29405310599528556 0.19315071326937425 >10 1.6464132558406723 2.3403272932285804 >50 0.23757055527417548 1.2487031124435186 >100 0.31680304822649996 5.26126041224169 >500 0.08946651261951015 4.652348073291905 >1k 0.1295153379533548 21.345812196130197 >5k 0.030613277558165054 15.735783827402635 >10k+ 0.024521326075495006 39.67718644746918 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 207953 0.6999829744515118 Illumina Single End PCR Primer 1 (100% over 50bp) GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG 133281 0.44863229103630115 No Hit CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC 119009 0.4005918347246732 No Hit CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG 105499 0.35511631869537846 No Hit GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA 100856 0.3394876865026312 No Hit GTCAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTGACTTTCGTTC 86671 0.2917400777035531 No Hit CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG 79917 0.26900568575226835 No Hit GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA 79680 0.2682079287353222 No Hit ATCAAACCGTACTCACTTCACATTACAGCCCGACCGGCCCAGTCCTTAGA 77528 0.2609641603789164 No Hit GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT 77161 0.25972881512482676 No Hit GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC 75651 0.25464605944723717 No Hit CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA 74134 0.24953974132610915 No Hit CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG 68883 0.23186454260887548 No Hit CTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTGGATC 68686 0.23120142812643496 No Hit GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG 66351 0.22334167017466572 No Hit GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC 65569 0.22070940862507957 No Hit CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT 64717 0.21784152264010853 No Hit GTCGATCCTAAGATATTGGCTAGTGCCGTTTTTTAAAATAAAATAGAGGA 64581 0.21738373802278918 No Hit CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC 64372 0.21668023077999699 No Hit GTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAATTAAAA 63976 0.21534726968839074 No Hit ATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAG 63404 0.21342188144495944 No Hit CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG 62182 0.2093085520158108 No Hit CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA 60867 0.20488217869393646 No Hit CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC 59130 0.19903532663302714 No Hit CGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGT 59111 0.19897137142913693 No Hit GAACAATCCAACGCTTGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCG 54498 0.18344372113726895 No Hit GCCAGATCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTA 54368 0.18300613290012546 No Hit CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC 53627 0.18051187994840767 No Hit CGGAGATAGGGATGTTGTTTCCGTAAAGCGCCACGGTTCCTGTGGTGTCT 53488 0.18004399714100042 No Hit CTCACTTCACATTACAGCCCGACCGGCCCAGTCCTTAGAGCCAATCCTTA 53020 0.17846867948728393 No Hit CTGGAATCGGTTCAGCCGGAGATAGGGATGTTGTTTCCGTAAAGCGCCAC 50386 0.16960246859008465 No Hit GTCGCAATGGTTAGCGTTGAAGACTTTGGGCGTAGGCCCGGTTGAAGCTT 49581 0.16689278758315776 No Hit CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG 49505 0.16663696676759696 No Hit CTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGA 48379 0.16284677942126194 No Hit CTCAGATGGTAGCTTCGCACCATTGGCCGATCGGCCAAGCACATGAACCA 48031 0.16167538937106252 No Hit GGCATTCGTATCGCTGCGTGAGAGGTGAAATTCTTGGACCGTAGCGAGAC 47349 0.1593797341577406 No Hit AAACAGTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAAT 46981 0.1581410228402883 No Hit CAACGGTTGAGTATAAGTCTCCCGCTCAAGCGCCATCCATTTTCAGGGCT 46513 0.1565657051865718 No Hit CGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCCACCAAAG 46211 0.15554915405105388 No Hit GTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTGGAT 46141 0.15531352961566894 No Hit GTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTAC 45087 0.1517656988314442 No Hit CTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCT 44482 0.1497292304970457 No Hit CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA 44459 0.14965181103970493 No Hit GTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCTAAGG 44169 0.14867565266453872 No Hit CACAGACCTGTTATTGCTCAACTTCATGCGGCTAAACACCGCTTGTCCCT 44124 0.14852417981321983 No Hit CCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTC 43488 0.14638336351457945 No Hit GGGAGTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCT 43387 0.14604339111495263 No Hit CACGCTTTCGCGGTCTGTATTCGTACTGGAAATCAAGATCAAGCGAGCTT 43347 0.14590874858044695 No Hit CTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAAC 42900 0.1444041182573459 No Hit GTCAATTTCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGAACCGA 41833 0.1408125286494068 No Hit CAGAAATCAGAGACAGTCAACGCCCGCAAGGGCCATCTGTCTCCTCTGTT 41221 0.13875249787146982 No Hit GTTTCCGTAAAGCGCCACGGTTCCTGTGGTGTCTCGTGCGCTTTGTTCGA 40278 0.13557830012049832 No Hit GACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTG 39303 0.13229638834192228 No Hit CGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGGTA 38843 0.13074799919510693 No Hit ATTGCTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGT 38751 0.13043832136574388 No Hit CTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTGC 38089 0.1282099874196748 No Hit CCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTT 37762 0.1271092847000908 No Hit GTTTACGGTCAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTGACT 37583 0.1265067593581779 No Hit CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT 37572 0.12646973266118883 No Hit CTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTT 37287 0.12551040460283583 No Hit GAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGC 37259 0.12541615482868185 No Hit GTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAATCACCG 36955 0.12439287156643865 No Hit ATTTTTTCAAGGTAAACTTGCTAGCCACCATATGCAACCAATTAAGGTAA 36772 0.12377688197107516 No Hit TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT 36513 0.12290507156015083 No Hit CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT 36055 0.12136341454006076 No Hit AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG 35909 0.12087196928911503 No Hit CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG 35549 0.11966018647856386 No Hit GATAAATCGAAAGGGAAACGGGTTAATATTCCCGTACCTGGACACGGAGA 35192 0.11845850185810064 No Hit GGGCATTCGTATCGCTGCGTGAGAGGTGAAATTCTTGGACCGTAGCGAGA 35014 0.11785934257955033 No Hit CTAGAGTCTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAAC 34580 0.11639847108016368 No Hit GTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTG 34250 0.11528767017049178 No Hit CTCGGATTTTCATGGATCGAACAAAGCGCACGAGACACCACAGGAACCGT 33970 0.11434517242895201 No Hit GTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAA 33708 0.11346326382793977 No Hit CTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTTCATGTGCTTGGC 33568 0.11299201495716987 No Hit GTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATTTTCATGGATC 33551 0.11293479188000496 No Hit CTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTTCGCACCA 33196 0.111739839386267 No Hit CTTGGACCGTAGCGAGACGTACGACTGCGAAAGCATTTGCCAAGAATGTC 33172 0.11165905386556359 No Hit CGAGACTCTAGCCTACTAAATAGTTACTGGATAATTTTATCGTCCAGACA 32941 0.11088149322879327 No Hit GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT 32908 0.11077041313782608 No Hit CTCACGTTGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTGCCC 32865 0.11062567241323247 No Hit GTTGAAGCTTCCATTGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAG 32831 0.11051122625890265 No Hit GTTCGATTAGTCTTTCGCCCCTATACCAAAGTCAGACGATCGATTTGCAC 32716 0.11012412897219881 No Hit CTGACTTTCGTTCTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCA 32681 0.11000631675450634 No Hit CTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAA 32312 0.1087642393736914 No Hit CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCC 31937 0.10750196561270062 No Hit CTCACGTTCCCTATTGGTGGGTGAACAATCCAACGCTTGGCGAATTCTGC 31746 0.10685904751043598 No Hit TGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGC 31532 0.10613870995083056 No Hit GCTGATTCCTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCAT 31293 0.10533422080715911 No Hit CCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTG 31237 0.10514572125885115 No Hit GGTAATTCCAGCTCTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGC 31215 0.10507166786487303 No Hit GTGTAATCCATCTGATAAATCGAAAGGGAAACGGGTTAATATTCCCGTAC 30878 0.10393730451166262 No Hit CTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAACCGAGATC 30411 0.10236535292130879 No Hit GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC 30374 0.10224080857689102 No Hit CCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGG 30104 0.10133197146897766 No Hit CTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATTC 29876 0.10056450902229525 No Hit CAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGG 29767 0.10019760811576728 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0012084167471885124 0.0 0.0 2 0.0012555416342655017 0.0 0.0 3 0.0013464253450568384 0.0 0.0 4 0.0015248267032768694 0.0 0.0 5 0.0016022461606176376 0.0 0.0 6 0.0016426389209693428 6.732126725284192E-6 0.0 7 0.001683031681321048 6.732126725284192E-6 0.0 8 0.0017974778356508792 6.732126725284192E-6 3.366063362642096E-6 9 0.0033694294260047377 6.732126725284192E-6 6.732126725284192E-6 10-11 0.004019079654994663 8.41515840660524E-6 6.732126725284192E-6 12-13 0.004357369022940193 1.0098190087926288E-5 6.732126725284192E-6 14-15 0.005663401607645327 1.0098190087926288E-5 6.732126725284192E-6 16-17 0.006886965639965728 1.1781221769247336E-5 6.732126725284192E-6 18-19 0.007502955235329232 1.3464253450568384E-5 1.0098190087926288E-5 20-21 0.008179533971220293 1.3464253450568384E-5 1.0098190087926288E-5 22-23 0.008739983521100201 1.3464253450568384E-5 1.0098190087926288E-5 24-25 0.009738021308123582 1.3464253450568384E-5 1.0098190087926288E-5 26-27 0.011698753216862603 1.5147285131889432E-5 1.0098190087926288E-5 28-29 0.01392372109956903 1.683031681321048E-5 1.0098190087926288E-5 30-31 0.027266796269082298 1.683031681321048E-5 1.0098190087926288E-5 32-33 0.03164941076724231 1.683031681321048E-5 1.0098190087926288E-5 34-35 0.03523258521677482 2.0196380175852575E-5 1.0098190087926288E-5 36-37 0.039675788855462385 2.0196380175852575E-5 1.0098190087926288E-5 38-39 0.044644098378722116 2.0196380175852575E-5 1.0098190087926288E-5 40-41 0.050536392295027106 2.0196380175852575E-5 1.0098190087926288E-5 42-43 0.057805406126652706 2.0196380175852575E-5 1.0098190087926288E-5 44-45 0.06635857313112628 2.0196380175852575E-5 1.0098190087926288E-5 46-47 0.07641300439533821 2.0196380175852575E-5 1.0098190087926288E-5 48-49 0.08872774720756432 2.187941185717362E-5 1.0098190087926288E-5 50-51 0.10342566288054103 2.6928506901136768E-5 1.0098190087926288E-5 52-53 0.12090562992274143 2.6928506901136768E-5 1.0098190087926288E-5 54-55 0.1421572709627823 2.6928506901136768E-5 1.0098190087926288E-5 56-57 0.16557329074500204 2.6928506901136768E-5 1.0098190087926288E-5 58-59 0.18974330872045364 2.6928506901136768E-5 1.3464253450568384E-5 60-61 0.21932427355135237 2.6928506901136768E-5 1.3464253450568384E-5 62-63 0.25331141532394963 2.8611538582457815E-5 1.3464253450568384E-5 64-65 0.29114260145668414 3.029457026377886E-5 1.3464253450568384E-5 66-67 0.33471292562272337 3.534366530774201E-5 1.3464253450568384E-5 68-69 0.38505913533776126 3.702669698906306E-5 2.6928506901136768E-5 70-71 0.44436748875583365 3.702669698906306E-5 2.8611538582457815E-5 72-73 0.5203260745972151 3.702669698906306E-5 3.029457026377886E-5 74-75 0.6136198867562035 3.702669698906306E-5 3.029457026377886E-5 76-77 0.7102208561689878 3.702669698906306E-5 3.029457026377886E-5 78-79 0.8254327899138201 3.702669698906306E-5 3.029457026377886E-5 80-81 0.9722234470952792 3.702669698906306E-5 3.029457026377886E-5 82-83 1.1359167914522457 3.702669698906306E-5 3.197760194509991E-5 84-85 1.3211680886152535 3.702669698906306E-5 3.366063362642096E-5 86-87 1.5463728748611416 3.870972867038411E-5 3.366063362642096E-5 88-89 1.8073976243805854 4.039276035170515E-5 3.366063362642096E-5 90-91 2.10012059258603 4.039276035170515E-5 3.534366530774201E-5 92-93 2.442149656927456 4.375882371434724E-5 3.702669698906306E-5 94-95 2.8513434665753614 4.712488707698934E-5 4.039276035170515E-5 96-97 3.3076874087754753 4.712488707698934E-5 4.375882371434724E-5 98-99 3.841031733427709 4.712488707698934E-5 4.375882371434724E-5 100-101 4.413365170009426 5.0490950439631436E-5 4.375882371434724E-5 102-103 5.058218758707586 5.0490950439631436E-5 4.375882371434724E-5 104-105 5.802149056421752 5.0490950439631436E-5 4.544185539566829E-5 106-107 6.64741637469994 5.3857013802273536E-5 4.712488707698934E-5 108-109 7.535431014652003 5.3857013802273536E-5 4.712488707698934E-5 110-111 8.496576747220827 5.3857013802273536E-5 4.712488707698934E-5 112-113 9.656473374068534 5.3857013802273536E-5 4.712488707698934E-5 114-115 10.846117585883594 5.3857013802273536E-5 4.880791875831039E-5 116-117 12.138275257407916 5.3857013802273536E-5 5.0490950439631436E-5 118-119 13.508986749626215 5.3857013802273536E-5 5.0490950439631436E-5 120-121 14.82081569544182 5.3857013802273536E-5 5.0490950439631436E-5 122-123 16.27494833597648 5.3857013802273536E-5 5.0490950439631436E-5 124-125 17.837614640544487 5.3857013802273536E-5 5.0490950439631436E-5 126-127 19.292317828819115 5.3857013802273536E-5 5.0490950439631436E-5 128-129 20.865400416462823 5.3857013802273536E-5 5.0490950439631436E-5 130-131 22.405426578853703 5.3857013802273536E-5 5.0490950439631436E-5 132-133 23.94065610095282 5.3857013802273536E-5 5.0490950439631436E-5 134-135 25.464424177302135 5.554004548359458E-5 5.2173982120952486E-5 136-137 27.02068654632272 5.722307716491563E-5 5.3857013802273536E-5 138-139 28.48433841404693 5.722307716491563E-5 5.3857013802273536E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAATC 10505 0.0 111.309975 2 GTCCAAT 11345 0.0 103.00453 1 CTTGTTA 18495 0.0 97.20602 1 ATCAAAG 14580 0.0 84.127625 6 GTTACGA 21705 0.0 82.66301 4 TCAAAGG 15025 0.0 82.456215 7 CAAAGGA 14530 0.0 81.57328 8 TTACGAC 22075 0.0 81.047615 5 CCAGACG 5715 0.0 79.27926 6 CAGACGT 6030 0.0 75.49847 7 CCAATCA 16425 0.0 71.76472 3 CAATCAA 18700 0.0 64.81723 4 CGCGGAT 1750 0.0 62.551064 1 CCTGGCC 16920 0.0 58.82555 5 GCGAGCG 3230 0.0 58.802334 1 TGTTACG 32210 0.0 55.455647 3 CAAACCG 21050 0.0 55.273724 3 TCAAACC 21800 0.0 53.57163 2 CCGGCAT 1740 0.0 53.32815 1 ACCTGGC 18705 0.0 53.250637 4 >>END_MODULE